Co-production process for extracting SOD and heme from chicken blood

A heme and blood technology, applied in the directions of hemoglobin/myoglobin, enzymes, chemical instruments and methods, etc., can solve the problems of environmental pollution, waste of protein resources, etc., and achieve the effect of high SOD activity

Inactive Publication Date: 2020-04-28
陈石良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most poultry blood in my country's slaughtering and processing industry is directly processed into ordinary protein feed raw materials, and a small a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Blood cell liquid preparation: 1000L of fresh chicken blood was collected in the chicken slaughtering factory. After anticoagulant treatment, it was continuously separated with a tubular centrifuge to obtain 280L of blood cell liquid and 720L of plasma liquid; the plasma liquid was spray-dried to make chicken plasma protein pink;

[0023] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 280L deionized water, and continuously stir for 60 minutes for hemolysis at a stirring speed of 150r / min to obtain 560L of hemolysis;

[0024] (3) Thermal denaturation reaction Ⅰ: adjust the pH value of hemolysis to 5.2 with HCl under stirring, rapidly raise the temperature to 60°C, keep warm for 60 minutes, rapidly cool down to below 40°C, and filter through a plate and frame filter press to obtain filtrates respectively Ⅰ 325L and blood residue Ⅰ 103 kg;

[0025] (4) Thermal denaturation reaction Ⅱ: Pump the filtrate Ⅰ into the reaction tank, adjust the pH v...

Embodiment 2

[0033] (1) Blood cell liquid preparation: Collect 1000L fresh chicken blood in the chicken slaughtering factory, after anticoagulant treatment, use a tubular centrifuge to continuously separate to obtain 300L blood cell liquid and 700L plasma liquid; the plasma liquid is spray-dried to make chicken plasma protein pink;

[0034] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 300L deionized water, and continuously stir for 60 minutes for hemolysis at a stirring speed of 150r / min to obtain 600L of hemolysis;

[0035] (3) Thermal denaturation reaction Ⅰ: adjust the pH value of hemolysis to 5.2 with HCl under stirring, rapidly raise the temperature to 60°C, keep warm for 60 minutes, rapidly cool down to below 40°C, and filter through a plate and frame filter press to obtain filtrates respectively Ⅰ 369L and blood residue Ⅰ 99 kg;

[0036] (4) Thermal denaturation reaction Ⅱ: Pump the filtrate Ⅰ into the reaction tank, adjust the pH value to 6.5 with NaOH, ...

Embodiment 3

[0044](1) Preparation of blood cell liquid: Collect 1000L of fresh chicken blood in the chicken slaughtering factory, after anticoagulant treatment, use a tubular centrifuge to continuously separate to obtain 265L of blood cell liquid and 735L of plasma liquid; the plasma liquid is spray-dried to make chicken plasma protein pink;

[0045] (2) Hemolysis: pump the blood cell solution into the reaction tank, add 260L deionized water, and stir continuously for 60 minutes for hemolysis at a stirring speed of 150r / min to obtain 520L of hemolysis;

[0046] (3) Thermal denaturation reaction Ⅰ: adjust the pH value of hemolysis to 5.2 with HCl under stirring, rapidly raise the temperature to 60°C, keep warm for 60 minutes, rapidly cool down to below 40°C, and filter through a plate and frame filter press to obtain filtrates respectively Ⅰ 331L and blood residue Ⅰ 82 kg;

[0047] (4) Thermal denaturation reaction Ⅱ: Pump the filtrate Ⅰ into the reaction tank, adjust the pH value to 6.5 ...

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Abstract

The invention discloses a co-production process for extracting SOD (Superoxide Dismutase) and heme from chicken blood, which comprises the following steps: by using chicken blood as a raw material, carrying out hemolysis, thermal denaturation reaction and filter pressing, and carrying out ultrafiltration concentration, purification and freeze drying on the filtrate to obtain SOD; and extracting the blood residue with acidic acetone, and carrying out separating and drying to obtain heme. By utilizing modern biochemical separation technologies such as thermal denaturation, isoelectric precipitation, ultrafiltration and extraction, the SOD and heme co-production process is formulated, and comprehensive utilization and high-valued development of chicken blood are realized. The process is freeof waste and pollution, short in production period and suitable for industrial production. The enzyme activity of the obtained SOD freeze-dried powder reaches 5000 U/mg or above, and the purity of theobtained heme reaches 85% or above.

Description

technical field [0001] The invention relates to the technical field of chicken blood deep processing, in particular to a co-production process for extracting SOD and hemoglobin from chicken blood. Background technique [0002] Poultry blood is a kind of nutritional resource rich in animal protein. It contains a variety of nutritional and biologically active substances, such as protein, amino acid, various enzymes, mineral elements, etc., with high potential nutritional value. As a big breeding country in the world, my country has extremely rich sources of poultry blood. According to preliminary statistics, at present, the annual slaughtering volume of chickens (including broilers and hens) in China is nearly 9 billion. Calculated on the basis of 100g of blood produced per chicken after slaughter, only With chicken blood, 9 million tons of blood can be collected every year, and the blood volume of hundreds of millions of other types of poultry (including ducks, geese, etc.) sl...

Claims

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Application Information

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IPC IPC(8): C07K14/805C12N9/02C12N1/34C12N1/14C12N1/36
CPCC07K14/805C12N9/0089C12Y115/01001
Inventor 陈石良
Owner 陈石良
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