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Method for increasing content of ginsenoside in ginseng adventitious roots by utilizing immobilized chaetomium globosum spores

A technology of ginsenosides and conchospores, which is applied in the field of tissue culture of traditional Chinese medicine ginseng, and achieves the effects of facilitating large-scale industrial production, short growth cycle and low production cost

Active Publication Date: 2020-05-05
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the direct application of live bacterial spores as elicitors

Method used

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  • Method for increasing content of ginsenoside in ginseng adventitious roots by utilizing immobilized chaetomium globosum spores

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Embodiment 1

[0020] The method for improving the content of ginsenosides in ginseng adventitious roots by utilizing immobilized chaete spores comprises the following steps:

[0021] (1) Add sucrose to the 3 / 4MS medium to make the final concentration 40g / L, add indolebutyric acid to make the final concentration 5mg / L, add 6-furfurylaminopurine to make the final concentration 0.1mg / L, and adjust the pH =6.5, sterilized to obtain liquid culture medium;

[0022] (2) Cut the adventitious roots of ginseng into small sections of 0.5-1 cm and inoculate them in conical flasks filled with 100 mL of the liquid medium obtained in step (1), and culture them at 25° C. and in the dark for 30 days;

[0023] (3) Take sodium alginate, add it to distilled water, make the mass concentration of sodium alginate 6%, stir to dissolve it completely, place at 4°C for 24h, sterilize at 121°C for 25min, cool down to room temperature, and sterilize with ultraviolet light for 30min to obtain Sodium alginate aqueous so...

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Abstract

The invention discloses a method for increasing the content of ginsenoside in ginseng adventitious roots by utilizing immobilized chaetomium globosum spores. The method comprises the following steps:(1) preparing a liquid culture medium; (2) culturing the ginseng adventitious roots in the liquid culture medium; (3) preparing a sodium alginate aqueous solution; (4) preparing a chaetomium globosumspore suspension; (5) uniformly mixing the sodium alginate aqueous solution with the chaetomium globosum spore suspension in an isopyknic manner so as to obtain a mixed solution, dropwise adding the mixed solution into a CaCl2 aqueous solution until particles are generated, carrying out filtering to obtain the particles, cleaning the particles with normal saline, and carrying out filtering so as to obtain immobilized chaetomium globosum spores; and (6) adding the immobilized chaetomium globosum spores into the culture medium in the step (2), continuing culturing for 5 days at 25 DEG C under adark condition, and carrying out harvesting. The method provided by the invention has the advantages of simple operation, short growth period, low production cost and the like. According to the method, accumulation of the ginsenoside is effectively promoted; the content of the ginsenoside in the ginseng adventitious roots is remarkably increased; and industrial large-scale production of the ginsenoside is facilitated.

Description

technical field [0001] The invention relates to a method for increasing the content of ginsenoside in adventitious roots of ginseng by using immobilized chaete spores, and belongs to the field of tissue culture of traditional Chinese medicine ginseng. Background technique [0002] Ginseng (Panax ginseng C.A.Mey.) is a plant of the genus Panax of Araliaceae. It is produced in Northeast China, North Korea, Japan, South Korea, and eastern Russia. Its rhizome is a valuable Chinese medicinal material, known as the "king of herbs". Ginsenoside is its main active ingredient, which has the functions of anti-oxidation, promoting blood circulation, lowering blood sugar, analgesia, anti-cancer and improving immunity. In recent years, ginseng has been widely used as a tonic in various health care products, including capsules (Shenyi Capsule, Ginseng Guipi Pill, etc.), beverages (ginseng source, royal jelly, etc.) and cosmetics. The market demand is increasing year by year, and the mark...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高文远王娟梁文霞许馨丹姚陆
Owner TIANJIN UNIV
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