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Application of deep-sea actinomycetes biological preparation in the preparation of drugs for the prevention of citrus canker disease

A technology for citrus ulcers and diseases, applied in the direction of chemicals for biological control, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as intolerance to storage, reduction of fruit commodity value, rot, etc., and achieve separation and preparation Easy, easy to expand production, low production cost

Active Publication Date: 2020-11-10
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seedlings and saplings are particularly severely damaged, resulting in fallen leaves and dead shoots, which will affect the tree vigor; the severely damaged fruit will drop fruit, and the light one will have disease scars, which will not tolerate storage and rot, which will greatly reduce the commodity value of the fruit and increase the cost of disease and pest control for fruit farmers. , loss of economic benefits

Method used

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  • Application of deep-sea actinomycetes biological preparation in the preparation of drugs for the prevention of citrus canker disease
  • Application of deep-sea actinomycetes biological preparation in the preparation of drugs for the prevention of citrus canker disease
  • Application of deep-sea actinomycetes biological preparation in the preparation of drugs for the prevention of citrus canker disease

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Materials and methods

[0019] 1. Materials

[0020] 1.1 Fermentation medium:

[0021] The preparation method of the fermentation medium is as follows: according to the mass fraction of 100%, it includes 0.5% of soluble starch, 0.5% of soybean powder, 2.0% of glucose, 0.2% of yeast extract powder, 0.2% of bacteriological peptone, KH 2 PO 4 0.05%, MgSO 4 ·7H 2 O 0.05%, coarse sea salt 0.3%, CaCO 3 0.2%, NaCl 0.4%, the balance is water, pH 7.2-7.4. According to the formula, the above-mentioned substances are mixed together and sterilized at 121°C for 20 minutes.

[0022] 2. Method

[0023] 2.1 Fermentation preparation of biological preparations

[0024] Seed cultivation: insert the mycelia or spores of deep-sea actinomycete Streptomyces niveus SCSIO 3406 into the above-mentioned fermentation medium, fill each 250mL bottle with 50mL fermentation medium, and cultivate on a shaking table at 28°C at a speed of 200rpm It takes about 36 hours to amplify the fermen...

Embodiment 2

[0026] Embodiment 2: the preventive experiment of fermented preparation to citrus canker disease (autumn shoots)

[0027] 1 Experimental conditions

[0028] 1.1 Selection of test objects, crops and varieties

[0029] Control object: citrus canker. Crops: citrus, Maogu tangerine.

[0030] 1.2 Environmental conditions

[0031] The cultivation conditions (soil type, low-lying degree, fertilizer, density, growth period) of all experimental plots were consistent, in line with local scientific agricultural practices. The cultivation conditions (soil type, low-lying degree, fertilizer, density, growth period) of all experimental plots were consistent, in line with local scientific agricultural practices.

[0032] 2 Experimental Design and Arrangement

[0033] 2.1 Positive control drug

[0034] 20% Zinc Thiazole SC, Zhejiang Xinnong Chemical Co., Ltd. 33.5% quinolinone SC, Sinochem Crop Protection Co., Ltd. 46% Copper Hydroxide WDG, DuPont, USA.

[0035] 2.2 Dosage

[0036] ...

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Abstract

The invention discloses an application of a deep sea actinomycete biological agent in preparation of drugs for preventing citrus ulcer diseases. Experiments show that the supernatant of fermentation liquor of deep sea actinomycetes Streptomyces niveus SCSIO 3406 has a good prevention effect on citrus ulcer diseases, and therefore the deep sea actinomycetes Streptomyces niveus SCSIO 3406 can be used for preventing and treating citrus ulcer diseases. The effect of the product provided by the invention is equivalent to or slightly better than that of part of commercial chemical pesticides, such as zinc thiazole and oxine-copper. The preparation method is low in production cost, energy-saving and environment-friendly, and is an ideal resource for developing novel natural medicines. The invention has the advantages of short production period, easiness in separation and preparation and easiness in expanded production.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to the application of a deep-sea actinomycete Streptomyces niveusSCSIO 3406 deep-sea actinomycete biological preparation in the preparation of medicines for preventing citrus canker disease (autumn shoots). Background technique [0002] Citrus canker is a plant quarantine target at home and abroad. The pathogen belongs to bacteria, which has the characteristics of high outbreak and difficult to prevent and cure. Once the fruit tree is infected, it basically gets sick every year. The disease affects citrus leaves, shoots and fruit. Seedlings and saplings are particularly severely damaged, resulting in fallen leaves and dead shoots, which will affect the tree vigor; the severely damaged fruit will drop fruit, and the light one will have disease scars, which will not tolerate storage and rot, which will greatly reduce the commodity value of the fruit and increase the c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/28A01P1/00C12N1/20C12R1/465
CPCC12N1/20
Inventor 宋永相杨佳凡赖添财鞠建华
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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