A method for fermenting and preparing long-chain dibasic acids
A long-chain dibasic acid and fermentation bacteria technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high hazards of microwave radiation and difficulties in practical application, so as to improve production intensity and reduce rejection , the effect of improving the utilization rate
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Embodiment 1
[0031] The medium formula is: sucrose 30g / L, corn steep liquor 2g / L, yeast extract 1.5g / L, sodium chloride 1.2g / L, potassium dihydrogen phosphate 3.5g / L, magnesium sulfate 1.8g / L, urea 4g / L L, ammonium sulfate 1.5g / L, sodium acetate 1.8g / L.
[0032] (1) Preparation of seed liquid: Inoculate 50 mL of fermented bacterial liquid into a 3L shake flask containing 450 mL of seed medium, culture at 32°C, shaker rotation speed at 120 rpm, and incubate for 48 hours to obtain fermented bacterial seed liquid.
[0033] (2) Pretreatment of alkanes: Take 750g of dodecane (about 1L in volume), add 750g of water and 15g of linoleic acid, and perform ultrasonic treatment for 10 minutes. The ultrasonic frequency is 40kHz and the power density is 50W / L.
[0034] (3) Long-chain dibasic acid fermentation: Add 500 mL of fermented seed liquid to 2.75 L of fermentation medium, and then add 1.75 L of dodecane pretreated in step (2). The fermentation parameters are as follows: the fermentation tempera...
Embodiment 2
[0037]The medium formula is: sucrose 35g / L, corn steep liquor 1.5g / L, yeast extract 1.8g / L, sodium chloride 0.8g / L, potassium dihydrogen phosphate 7.5g / L, magnesium sulfate 1.2 / L, urea 4.8g / L, ammonium sulfate 2g / L, sodium acetate 1.5g / L.
[0038] (1) Preparation of seed liquid: Inoculate 50 mL of fermented bacterial liquid into a 3L shake flask containing 950 mL of seed medium, culture at 28°C, shaker rotation speed at 120 rpm, and incubate for 48 hours to obtain fermented bacterial seed liquid.
[0039] (2) Pretreatment of alkanes: Take 1875g of tridecane (about 2.5L in volume), add 3750g of water and 37.5g of oleic acid, and perform ultrasonic treatment for 20 minutes at a frequency of 30kHz and a power density of 20W / L.
[0040] (3) Fermentation of long-chain dibasic acid: add 1L of fermented seed liquid to 2.75L of fermentation medium, and then add 6.25L of dodecane pretreated in step (2). The fermentation parameters are as follows: the fermentation temperature is 28°C,...
Embodiment 3
[0043] The formula of seed medium is: sucrose 30g / L, corn steep liquor 2g / L, yeast extract 1.5g / L, sodium chloride 1.2g / L, potassium dihydrogen phosphate 3.5g / L, magnesium sulfate 1.8 / L, urea 4g / L L.
[0044] The formula of the fermentation medium is: sucrose 30g / L, corn steep liquor 2g / L, yeast extract 1.5g / L, sodium chloride 1.2g / L, potassium dihydrogen phosphate 3.5g / L, magnesium sulfate 1.8 / L, urea 4g / L L, ammonium sulfate 1.5g / L, sodium acetate 1.8g / L.
[0045] (1) Preparation of fermented seed liquid: Inoculate 50 mL of fermented bacterial liquid into a 3L shake flask containing 450 mL of seed medium, culture at 30°C, shaker rotation speed at 120 rpm, and cultivate for 48 hours to obtain fermented bacterial seed liquid.
[0046] (2) Pretreatment of alkanes: Take 750g of dodecane (about 1L in volume), add 1500g of water and 7.5g of oleic acid, and perform ultrasonic treatment for 20 minutes. The ultrasonic frequency is 25kHz and the power density is 50W / L.
[0047] (3) ...
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