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Application of frenolicin B in inhibition of activity of pathogenic fungi causing plant diseases

A technology of pathogenic fungi, phelicomycin, applied in the field of pesticides, can solve problems such as ecological damage, environmental pollution, and enhanced drug resistance of pathogenic bacteria, and achieve excellent control effects, significant control effects, and broad antibacterial spectrum effects

Active Publication Date: 2020-05-12
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of chemical control is single, and long-term excessive use has caused serious environmental pollution and ecological damage, and repeated use has led to enhanced drug resistance of pathogenic bacteria

Method used

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  • Application of frenolicin B in inhibition of activity of pathogenic fungi causing plant diseases
  • Application of frenolicin B in inhibition of activity of pathogenic fungi causing plant diseases
  • Application of frenolicin B in inhibition of activity of pathogenic fungi causing plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Mycelia growth inhibition test.

[0023] The inhibitory effect of Fulinmycin on wheat scab and various plant pathogenic fungi was determined indoors.

[0024] The plant pathogens used in this implementation are 15 common plant pathogenic fungi in agricultural production, and the strains and sources are as follows: Fusarium graminearum (Fusarium graminearum) was provided by Zhejiang University; Mildew fungus (Phytophthora capsici), provided by Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences; corn small spot fungus (Helminthosporium maydis), corn big spot fungus (Setosphaeriaturcica turcicaf), corn smut fungus (Sphacelotheca reiliana), corn curvularia fungus ( Curvularia lunata), Fusarium oxysporum f.sp. tracheiphilum, Sclerotinia sclerotiorum, Phytophthora sojae, Fusarium oxysporum f.sp. cucumerinum, Melon Fusarium oxysporum f. sp. melonis, Stagonosporopsis cucurbitacearum, Fusarium oxysporum and Fusarium fujikuroi were pro...

Embodiment 2

[0030] Example 2. In vitro test of spraying Fulinmycin to control wheat scab.

[0031] Medicament: 38 mg / L Fulinmycin, added with a final concentration of 0.05% Tween 20 (to increase surface adhesion).

[0032] Control drug: active ingredient 750mg / L cyclostrobin (Jinghu—25% cyclostrobin suspension), active ingredient 750mg / L carbendazim (super blue powder—80% wettable powder);

[0033] Infected control: clear water

[0034] Treatment method: At the flowering stage of 5% wheat, select wheat ears with consistent growth, cut them off about 10cm below the flag leaf node, remove the flag leaf leaves, insert them into sterile water, and spray them for control. After 24 hours, inoculate the spores of Gibberella (final concentration 104 conidia / mL) (adding a final concentration of 0.05% Tween 20 to increase surface adhesion), the spray volume per ear is about 1 mL. Each treatment was inoculated with 20 ears, and each treatment was repeated 3 times. After inoculation, the plants wer...

Embodiment 3

[0041] Example 3. In vitro test on the control of wheat head blight by instilling Fulinmycin into a single flower.

[0042] Medicament: the medicament is mixed with scab spores (final concentration 10 4 conidia / mL) mixed, the final concentration of each agent: 38mg / L Fulinmycin, add a final concentration of 0.05% Tween 20 (to increase surface adhesion);

[0043] Control agent: 750 mg / L of active ingredient of cyclostrobin (Jinghu—25% cyclostrobin suspension), 750 mg / L of active ingredient of carbendazim (super blue powder—80% wettable powder).

[0044] Treatment method: At the flowering stage of 5% wheat, select wheat ears with consistent growth, cut them off about 10cm below the flag leaf node, remove the flag leaf leaves, insert them into sterile water, and use a pipette to reach the spikelets in the middle of the ears. Inject 10 μl of the sample into the glume. Each treatment was inoculated with 20 ears, and each treatment was repeated 3 times. After inoculation, they wer...

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Abstract

The invention discloses an application of frenolicin B in inhibition of the activity of pathogenic fungi causing plant diseases, belonging to the technical field of pesticides. In order to develop a novel wheat scab prevention and treatment means without pollution to the environment and the ecological system, the invention provides application of frenolicin B in inhibition of the activity of pathogenic fungi causing plant diseases. Experiments show that frenolicin B has broad-spectrum antibacterial activity on a plurality of plant pathogenic fungi including Setosphaeriaturcica turcicaf, Helminthosporiummaydis, Curvularia lunata, Sphacelotheca reiliana, Phytophthora sojae, Sclerotinia sclerotiorum, Fusarium oxysporumf.sp.tracheiphilum, Rhizoctonia solani, Fusarium oxysporum, Fusariumfujikuroi, Phytophthora capsici, Fusarium oxysporum f.sp.melonis, Fusarium oxysporum f.sp.cucumerinum and Stagonosporopsis cucurbitacearum. The frenolicin B has obvious inhibition effect on the fusarium graminearum and can be used for preventing and treating pathogenic fungi of field crops.

Description

technical field [0001] The invention specifically relates to the application of Fulinmycin in inhibiting the activity of pathogenic fungi that cause plant diseases, and belongs to the technical field of pesticides. Background technique [0002] Fusarium head blight (FHB) caused by Fusarium fungus infection is a serious worldwide fungal disease. It can be infected at various growth stages of plants. It can cause seedling blight at the seedling stage, and can cause ear rot of wheat and barley and corn rot at the mature stage. Wheat head blight is a typical climatic fungal disease, mainly distributed in temperate and subtropical regions with humid and rainy climate. With frequent global exchanges, head blight has gradually spread from East Asia, South America and southern Africa to North America and Europe, and has gradually become a worldwide wheat disease. In China, wheat scab was reported for the first time in 1936, and the most serious occurrence area is the wheat region ...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P3/00
CPCA01N43/90
Inventor 向文胜刘重喜韩传玉余志银王相晶
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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