Application of sulfated fucoidan as PCSK9 inhibitor
A sulfated fucoidal oligosaccharide and sulfated technology, which is applied in the fields of application, sugar production, sugar production, etc., can solve problems such as LDL cannot be effectively removed, cost-benefit asymmetry, hypercholesterolemia, etc.
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Embodiment 1
[0036] Extracting and preparing sulfated fucoidal oligosaccharides from Ascophyllum nodosum, the following steps were taken:
[0037](1) Preparation of the crude product of Ascophyllum nodosum fucoidan: wash the Ascophyllum nodosum, soak to remove salt, pulverize with a pulverizer, dry at 60°C, pulverize again, and pass through a 100-mesh sieve. Add 3 times the volume of petroleum ether to the crushed and dried Ascophyllum nodosum, and reflux at 90°C to remove most of the pigments and lipids. The filter residue was dried, ground, extracted by water extraction, heated in a water bath at 90°C for 3 hours, and centrifuged to obtain the supernatant and filter residue (repeated water extraction of the filter residue 3 times). The supernatant was collected and then concentrated to one-third of the original volume. Calculation of the volume of the concentrated solution needs to add the amount of calcium chloride to make the final concentration reach 2%. Stir while adding. At this tim...
Embodiment 2
[0054] When extracting and preparing sulfated fucoidan oligosaccharides from sea cucumbers and verifying their PCSK9-inhibiting and lipid-lowering activities, the following steps were taken:
[0055] (1) Preparation of crude sea cucumber fucoidan: Sea cucumbers were washed to remove sediment, soaked to remove salt, pulverized by a pulverizer, dried at 50°C, pulverized again, and passed through a 200-mesh sieve. Add 5 times the volume of ethyl acetate to the crushed and dried sea cucumber, and reflux at 95°C to remove most of the pigment and lipid. The filter residue is dried, ground, and extracted with papain (the mass ratio of sea cucumber extract is 1:5-1:10): add 100 mg of papain to each liter of extract, and adjust the pH value of the solution with sodium acetate and hydrochloric acid To 5.6, heat overnight in a 60°C water bath, and centrifuge to obtain the supernatant. Collect the supernatant and then concentrate it to one-third of the original volume, add 95% ethanol un...
Embodiment 3
[0066] To extract and prepare kelp sulfated fucoidan oligosaccharides, the following steps were taken:
[0067] (1) Preparation of crude kelp fucoidan: wash the kelp to remove sediment, soak to remove salt, pulverize with a pulverizer, dry at 40°C, pulverize again, and pass through a 100-mesh sieve. Add 10 times the volume of petroleum ether to the crushed and dried kelp, condense and reflux at 93°C to remove most of the pigments and lipids. The filter residue was dried, ground, extracted by water extraction, heated in a water bath at 100°C for 2 hours, and centrifuged to obtain the supernatant and filter residue (repeated water extraction of the filter residue 3 times). The supernatant was collected and then concentrated to one-third of the original volume. Calcium chloride needs to be added to calculate the volume of the concentrated solution so that the final concentration reaches 2%. Stir while adding. At this time, a white flocculent precipitate will appear, which is algi...
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