Facial anti-wrinkle freeze-dried powder containing SNAP25-Htt150Q fusion gene nano-liposome, and preparation method thereof

A nano-liposome, snap25-htt technology, applied in the field of medical cosmetology

Pending Publication Date: 2020-05-26
广州优理氏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet, also do not have a real and effective topical anti-wrinkle cosmetics on the market at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] A preparation method for facial anti-wrinkle freeze-dried powder containing SNAP25-Htt150Q fusion gene nano liposome, comprising the following steps:

[0029] S1, extracting the SNAP25-Htt fusion gene, the specific extraction method is as follows:

[0030] First, the first exon of the Htt gene of 150Q is fused with the SNAP25 gene;

[0031] Secondly, the PRK5 vector was transformed, and the mouse prion promoter and Pmel and Spel restriction sites were inserted, and then the SNAP25-Htt fusion gene was constructed on the transformed PRK5 vector;

[0032] Again, transform the plasmid vector into TOP10 competent cells, pick 3-5 clones after being coated with ampicillin-resistant plates for 24 hours, shake the bacteria, extract the plasmid with a small plasmid extraction kit, and use Pmel and Spel to extract 3-5 clones. The plasmids identified by enzyme digestion were identified by enzyme digestion. The identification primers for enzyme digestion identification were divided...

Embodiment 1

[0039] Effect experiment: 200 healthy women aged 35-45 were selected for a 30-day experiment. 200 people were randomly divided into 2 groups, 100 people in each group. Set as control group and experimental group. The control group used lyophilized powder containing only mannitol, and the experimental group used lyophilized powder containing SNAP25-Htt150Q fusion gene. After 30 days, the anti-wrinkle effect around the mouth and orbit of the test personnel was tested with a visa measuring instrument. Wrinkles are measured by the following three criteria.

[0040] Superficial wrinkles: mild degeneration of elastic tissue, that is, subtle tissue changes with slightly deepened skin lines.

[0041] Severe wrinkles: Moderate degeneration of elastic tissue, that is, obvious papular elastic tissue degeneration, yellow translucent papules, and skin discoloration.

[0042] Severe wrinkles: Severe degeneration of elastic tissue, that is, multipapular and fused elastic tissue degenerat...

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Abstract

The invention discloses a facial anti-wrinkle freeze-dried powder containing an SNAP25-Htt150Q fusion gene nano-liposome, and a preparation method thereof. The freeze-dried powder comprises, by mass,0.1% of the SNAP25-Htt fusion gene nano-liposome, 5.5% of mannitol, and the balance of sterile water, and the SNAP25-Htt fusion gene nano-liposome is composed of an SNAP25-Htt fusion gene, sterile water, soybean lecithin, cholesterol and polyvinyl alcohol. The invention also discloses a preparation method of the facial anti-wrinkle freeze-dried powder containing the SNAP25-Htt150Q fusion gene nano-liposome. The SNAP25-Htt150Q fusion gene can be easily transferred to a synaptic part to play a role, and finally, is processed in a vacuum freeze-drying manner to prepare the freeze-dried powder, sothat the activity of the SNAP25-Htt150Q fusion gene can be maintained for a long time, and the SNAP25-Htt150Q fusion gene has good anti-wrinkle effect.

Description

technical field [0001] The invention relates to the technical field of medical cosmetology, in particular to a face anti-wrinkle freeze-dried powder containing SNAP25-Htt150Q fusion gene nano liposome and a preparation method thereof. Background technique [0002] Skin wrinkles are the most important feature of female skin aging, and the earliest and most obvious part of aging symptoms is the face. With the rapid development of science and technology, the most effective way of facial wrinkle removal has gone from traditional wrinkle removal to the current minimally invasive injection wrinkle removal, and the technology is becoming more and more mature and advanced. However, both traditional incision and minimally invasive surgical interventions bring many uncontrollable risks to patients. [0003] The aging of facial skin is closely related to the excessive activity of facial expression muscles. When facial muscles contract, the facial skin will also move accordingly, formi...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K9/19A61K9/127A61K47/32A61K47/26A61P17/18A61K8/60A61K8/02A61K8/14A61K8/81A61K8/34A61Q19/08
CPCA61K48/005A61K9/19A61K9/1271A61K47/32A61K47/26A61P17/18A61K8/606A61K8/022A61K8/14A61K8/8129A61K8/345A61Q19/08A61K2800/84A61K2800/56A61K2800/413
Inventor 李占鸿
Owner 广州优理氏生物科技有限公司
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