Porous material for the detection of candida albicans, diagnostic method using same and preparation method thereof
A technology of Candida albicans and porous materials, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as high cost and slow speed, achieve low manufacturing cost, improve positioning, and facilitate medical diagnosis and decision-making Effect
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example 1
[0104] Example 1 : Synthesis of Mesoporous Silicon Nanoparticles (MSNs)
[0105] Mesoporous silicon nanoparticles (MSNs) were synthesized using a surfactant (cetyltrimethylammonium bromide, CTABr) and a silicon source (tetraethoxysilane, TEOS). For this, CTABr (1.00 g, 2.74 mmol) was first dissolved in 480 ml deionized water. Next, NaOH (2.00M, 3.50ml) was added to the previous solution, and the temperature was adjusted to 80°C. Next, TEOS (5.00ml, 25.7mmol) was added dropwise to the previous solution. The mixture was kept stirring for 2 hours, a white precipitate was obtained. The resulting powder was centrifuged and washed with deionized water. Finally, the solid was dried at 60 °C. To prepare the final porous support, the material was calcined at 550 °C for 5 h in an oxidizing atmosphere to remove the surfactant.
example 2
[0106] Example 2 : Synthesis of material S1
[0107] 200 g of calcined MSN (diameter 50-150 nm, total surface 1200 m 2 / g) was suspended in a solution containing 766.4 mg (0.16 mmol) of rhodamine B in 10 ml of acetonitrile, and the mixture was stirred at room temperature for 24 hours. Next, 247 l (1 mmol) of (3-propylisocyanate)triethoxysilane was added, and allowed to react at room temperature for 5.5 hours. Next, the mixture was vacuum filtered, washed with acetonitrile, and dried in a heater at 65°C. The obtained solid was characterized using thermogravimetric, TEM and X-ray powder diffraction techniques and identified as material S1. figure 1 The obtained mesoporous inorganic matrix (1) loaded with a dye / fluorophore (rhodamine B) (2) and functionalized with neutral organic groups is shown.
example 3
[0108] Example 3 : Synthesis of material S2
[0109] 1 mg of material S1 was suspended in 700 l of acetonitrile solution with rhodamine B (1 mM), to which oligonucleotide O1 (SEQ No. 1) (NH 2 -(CH 2 ) 6 -5'-AAA AAA CCC-3'). 2 l of triethylamine were added to the mixture and allowed to stir at room temperature for 3 hours. Next, the suspension was centrifuged at 12,000 rpm for 2 min, and the liquid was separated and washed with 1 ml of pH 7.5 aqueous buffer solution (MgCl 2 37.5mM, Tris-HCl 20mM) to wash the solid. The suspension was again centrifuged to obtain S2 material. figure 2 The obtained solid is shown, which was characterized by employing thermogravimetric, TEM and X-ray powder diffraction techniques. as stated figure 2 As shown, the porous material (1) was loaded with dye / fluorophore (rhodamine B) (2) and functionalized with oligonucleotide O1 (SEQ No.1) (3), oligonucleotide O1 (SEQ No. .1)(3) Covalent anchoring via urea functional groups. The concentrat...
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