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Application of lactobacillus capable of efficiently removing ammonia nitrogen

A lactobacillus, high-efficiency technology, applied in the field of microorganisms, can solve the problems of inability to quickly deodorize urine, inability to efficiently decompose ammonia nitrogen, etc.

Active Publication Date: 2020-06-02
浙江亲水园生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem that the existing lactobacilli cannot efficiently decompose ammonia nitrogen and quickly deodorize urine, the purpose of the present invention is to provide a new lactobacillus, so that the lactobacilli can efficiently decompose ammonia nitrogen and effectively treat various types of lactobacilli. Ammonia nitrogen wastewater

Method used

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  • Application of lactobacillus capable of efficiently removing ammonia nitrogen
  • Application of lactobacillus capable of efficiently removing ammonia nitrogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Acid resistance test: use QV1 freeze-dried bacterial powder (10 11 cfu / g), diluted 100-fold with warm water at 37°C and left for 0.5 hours, put into the MRS liquid medium whose pH value had been adjusted to 3.0 and 2.0 (using hydrochloric acid) respectively, and measured the activity after 1 hour and 2 hours, respectively. Bacterial count, the following results were obtained:

[0037] pH value before testing 1 hour after adding strong acid 2 hours after adding strong acid pH 3.0 5*10(8) 4*10(8) 6*10(7) pH 2.0 5*10(8) 6*10(7) 5*10(6)

[0038] Note: 5*10(8) in the above table means 5 multiplied by 10 to the 8th power of viable bacteria, and the same applies to the rest.

Embodiment 2

[0040] Salt tolerance test: using QV1 freeze-dried bacterial powder (10 11 cfu / g), diluted 100 times with warm water at 37°C and placed for 0.5 hours, then put into the MRS liquid medium whose bile salt concentration had been adjusted to 0.1% and 0.3%, respectively, and measured the viable bacteria after 1 hour and 2 hours number, the following result is obtained:

[0041] before testing 1 hour after addition of bile salts 2 hours after addition of bile salts Bile salts 0.1% 5*10(8) 3.5*10(8) 2*10(8) Bile salts 0.3% 5*10(8) 2*10(7) 1.2*10(6)

[0042] Note: 5*10(8) in the above table means 5 multiplied by 10 to the 8th power of viable bacteria, and the same applies to the rest.

Embodiment 3

[0044] The high temperature test uses QV1 freeze-dried bacterial powder (10 11 cfu / g), diluted 100 times with warm water at 37°C and left for 0.5 hours, then placed in MRS liquid culture medium heated to 80°C and 100°C respectively, and measured the number of viable bacteria after 10 minutes and 30 minutes respectively, and obtained the following result:

[0045] before testing 1 hour later 2 hours later 80℃ hot water 5*10(8) 4.5*10(8) 2.5*10(8) 100℃ hot water 5*10(8) 1*10(8) 2*10(7)

[0046] Note: 5*10(8) in the above table means 5 multiplied by 10 to the 8th power of viable bacteria, and the same applies to the rest.

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Abstract

The invention discloses an application of lactobacillus capable of efficiently removing ammonia nitrogen, and belongs to the field of microorganisms. The lactobacillus provided by the invention is a lactobacillus sp. strain QV1 with a preservation number CGMCC No.16350, and has the characteristics of acid resistance, salt resistance, high temperature resistance, and capability of been propagated under anaerobic, aerobic and facultative states. The lactobacillus provided by the invention can be applied in the fields of various sewage treatment (particularly various high ammonia nitrogen sewagetreatment) and the like.

Description

technical field [0001] The invention belongs to the field of microorganisms, and more specifically relates to a new lactobacillus and its application, especially the application in the treatment of various high ammonia nitrogen sewage. Background technique [0002] Lactobacillus belongs to Lactobacillus family and is named for the large amount of lactic acid produced by fermenting sugar. Widely distributed in nature, it is one of the important physiological flora in the intestinal tract of animals and humans. The vast majority of bacteria in this group are non-toxic and harmless to animals and humans, and they are responsible for important physiological functions in animals. Among lactic acid bacteria, Lactobacillus is the largest genus, which is defined as Lactobacillus rod. The genus Lactobacillus is most closely related to animals and is one of the dominant flora in the gut and vagina of both animals and humans. Lactobacillus is widely distributed, and animals and huma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A23K10/18C05F15/00C12R1/225C02F101/16C02F103/16
CPCC02F3/34A23K10/18C05F3/00C02F2101/16C02F2103/16C12R2001/225C12N1/205C05F11/08
Inventor 丁庆曾筱芳
Owner 浙江亲水园生物科技有限公司