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Trichoderma harzianum TW21990 for preventing and treating gray mold of leek and application of trichoderma harzianum TW21990

A technology for botrytis cinerea and chives, which is applied to biocontrol strains and their application fields, and can solve the problems of difficulty in ensuring the safe interval of pesticides, reducing economic benefits, and excessive drug residues.

Active Publication Date: 2020-06-12
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the China Pesticide Information Network, only 15% procymidone smoke agent and 50% procymidone wettable powder are the legally registered pesticide products for the prevention and treatment of gray mold of leek. The period generally ranges from 25 to 40 days. If the harvest standard is not harvested in time, it will affect the commerciality and reduce the economic benefits. Therefore, it is difficult to guarantee the safe interval of pesticides. This means that once procymidone is applied in the production of leeks in facilities , face the risk of excessive drug residues
[0003] In the current prior art, the use of Trichoderma to prevent and control gray mold of cucumber and tomato has been better applied, but the effect is not good in the prevention and treatment of gray mold of Chinese chives. Adaptive bacterial agents and application technology are the fundamental way to solve drug residues in leeks

Method used

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  • Trichoderma harzianum TW21990 for preventing and treating gray mold of leek and application of trichoderma harzianum TW21990
  • Trichoderma harzianum TW21990 for preventing and treating gray mold of leek and application of trichoderma harzianum TW21990
  • Trichoderma harzianum TW21990 for preventing and treating gray mold of leek and application of trichoderma harzianum TW21990

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A strain of Trichoderma harzianum TW21990 that prevents gray mold of leeks, named Trichoderma harzianum Trichodermaharzianum, referenced biological material: TW21990, preserved in the General Microbiology Center of China Microbial Culture Collection Management Committee, address: Beichenxi, Chaoyang District, Beijing No. 3, No. 1 Courtyard, Zip Code: 100101, its deposit number is: CGMCCNo.12864, and the deposit date is September 1, 2016. After morphological and ITS sequence analysis, it was identified as Trichoderma harzianum.

[0025] For the colony characteristic photos of Trichoderma strain TW21990, please refer to the attached picture of the manual figure 1 As shown, please refer to the accompanying drawing for the photomicrograph of conidiophores figure 2 As shown, please refer to the attached picture of the conidia micrograph image 3 shown.

[0026] Trichoderma strain TW21990ITS sequence, Tef1 sequence, Rpb2 sequence of the present invention

[0027] TW21990...

Embodiment 2

[0034] Plate Screening of Trichoderma Strains for Control of Chive Botrytis

[0035] Trichoderma strain Trichoderma harzianum TW21990 and pathogenic fungi with a diameter of 5 mm were inoculated at the two ends of the PDA plate at a distance of 35 mm from the center. Inoculate only the pathogenic fungus as a control, culture at 25°C for 3-5 days, record the growth radius of the pathogenic fungus in the confrontation direction, and calculate the inhibition Rate(%).

[0036] Inhibition rate (%)=100%×(growth radius of control bacteria-growth radius of treated bacteria) / growth radius of control bacteria.

[0037] As attached in the manual Figure 4 As shown, the results are shown in Table 1:

[0038] Table 1 Plate inhibitory effect of Trichoderma harzianum TW21990 strain on Botrytis cinerea (20°C)

[0039]

[0040] As can be seen from the table, the inhibition rate of Trichoderma harzianum TW21990 to Botrytis cinerea plate was 97.00% in 6 days, compared with control bacteria...

Embodiment 3

[0042] Trichoderma harzianum TW21990 was fermented to prepare conidia parent drug, and to prepare flush-type Trichoderma powder and spray-type Trichoderma powder.

[0043] A spray-type wettable powder, characterized in that Trichoderma harzianum TW21990 is fermented to prepare the conidia parent drug, adding 2%-5% of trehalose and 0.1%-0.5% of xanthan gum according to the weight percentage of the parent drug , 93%-96% of diatomite is prepared into a spray-type Trichoderma powder, and the effective spore content is 200 million / gram.

[0044] A Trichoderma powder for flushing application, which is prepared by fermenting Trichoderma harzianum TW21990 conidia parent drug, adding 90%-95% of peat soil or 90%-95% of medical stone according to the weight percentage of the parent drug to prepare the flushing type Trichoderma powder. Type Trichoderma powder, the effective spore content is 200 million / g

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Abstract

The invention discloses trichoderma harzianum TW21990 for preventing and treating gray mold of leek and an application of the trichoderma harzianum TW21990. The trichoderma harzianum TW21990 is preserved in China General Microbiological Culture Collection Center, a preservation number of the trichoderma harzianum TW21990 is CGMCC No.12864, and the preservation date of the trichoderma harzianum TW21990 is September 1, 2016. The leek gray mold pathogenic bacteria botrytis cinerea are used as targets; the efficient antagonistic trichoderma is screened by adopting a flat plate antagonistic mode; the trichoderma harzianum strain TW21990 for efficiently preventing and treating the gray mold of the leek is obtained by adopting field plot trial secondary screening, the prevention and treatment effect of plots on the gray mold of the leek is 90% or above, a preparation used for flushing application and spraying is developed, and the effect of preventing and treating the gray mold of the leek is85% or above when the preparation is applied to fields.

Description

technical field [0001] The invention relates to the technical field of biocontrol bacterial strains and applications thereof, in particular to a Trichoderma harzianum TW21990 for preventing and treating gray mold of leeks and its application. Background technique [0002] Botrytis cinerea of ​​leek, caused by the semi-known fungus Botrytis subphylum Botrytis genus Botrytis, occurs seriously in low temperature and high humidity environmental conditions in winter protected areas, ranging from a 10-30% reduction in yield to severe shed destruction. According to the China Pesticide Information Network, only 15% procymidone smoke agent and 50% procymidone wettable powder are the legally registered pesticide products for the prevention and treatment of gray mold of leek. The period generally ranges from 25 to 40 days. If the harvest standard is not harvested in time, it will affect the commerciality and reduce the economic benefits. Therefore, it is difficult to guarantee the safe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01N25/12C12R1/885
CPCA01N25/12C12N1/14
Inventor 李纪顺孙作文陈凯扈进冬魏艳丽王贻莲石朝鹏王丹李红梅
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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