Meniscus composite scaffold and preparation method thereof
A composite scaffold and meniscus technology, applied in medical science, prostheses, elbow joints, etc., to achieve the effects of protecting biological activity, achieving orderly slow release, and good mechanical properties
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[0042]The embodiment of the first aspect of the present invention provides a method for preparing a meniscus composite bracket, the method comprising:
[0043] S100, preparing a plurality of composite layers stacked in sequence to obtain a preformed body of a meniscal composite scaffold, wherein the composite layer includes a plurality of scaffold fibers arranged at intervals and a hydraulic gel between adjacent scaffold fibers Glue material, the scaffold fibers contain degradable polymer materials, the hydrogel slurry contains matrix materials and platelet-derived growth factor PDGF, and the scaffold fibers of two adjacent composite layers form a preset angle;
[0044] S200, cross-linking and curing the hydrogel slurry in the preform to obtain a meniscus composite bracket.
[0045] The invention simulates the spatial arrangement of normal meniscal collagen fibers by using degradable macromolecule material support fibers to construct a support frame, so that the meniscus compo...
Embodiment 1
[0125] 1) Preparation of dMECM: Fresh porcine meniscus was cut off excess synovial tissue, and cut into fragments with a volume of roughly 1 mm×1 mm×1 mm. Put the meniscus fragments into a sterile container, rinse with medical hydrogen peroxide (3%) 3 times, 5 min each time; then rinse 3 times with PBS, 5 min each time. Add the aseptic meniscus fragments into triple distilled water, and use a homogenizer to break into suspension slurry. The concentration of the suspended slurry was 1% w / v. In the RC-6+ low-temperature high-speed centrifuge, centrifuge the suspended slurry at 1500rpm at 4°C for 5min to separate large suspended particles; then take the supernatant and centrifuge at 2000rpm at 4°C for 15min, and take the supernatant again The solution was centrifuged at 6,000 rpm for 20 min at 4°C, and finally the supernatant was centrifuged at 10,000 rpm for 30 min at 4°C to collect the precipitate. Then the precipitate was mixed with sterile PBS, and centrifuged at 10,000 rpm...
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