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Probiotics agent for multi-strain combined solid-state fermentation of rapeseed meal and a fermentation method of probiotics agent

A technology of solid-state fermentation and fermentation method, which is applied in food processing, animal husbandry, food science, etc., to achieve the effects of increased peptide content, high efficiency, and simple operation

Pending Publication Date: 2020-06-19
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on a microbial combination solid-state fermentation method with good ability to remove glucosinolates, tannins, phytic acid, crude cellulose and increase peptide content at the same time

Method used

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  • Probiotics agent for multi-strain combined solid-state fermentation of rapeseed meal and a fermentation method of probiotics agent
  • Probiotics agent for multi-strain combined solid-state fermentation of rapeseed meal and a fermentation method of probiotics agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Pretreatment and sterilization of rapeseed meal raw materials: the rapeseed meal was oven-dried at 45°C for 4 hours, crushed through a 60-mesh sieve, and sterilized by high-pressure steam at 121°C for 20 minutes.

[0030] (2) Preparation of fermented seed liquid: adopt the shake flask culture method to ferment and cultivate Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis respectively, wherein the fermentation medium used by Bacillus amyloliquefaciens and Bacillus subtilis is broth medium, brewing The fermentation medium used by the yeast is potato-glucose medium, both of which are liquid medium; after being sterilized by high pressure steam at 121°C for 20 minutes, it is cooled to room temperature for later use; For several phases, inoculate the test tube liquid into the fermentation medium of the three strains with an inoculation amount of 1% by volume. The culture temperature of Saccharomyces cerevisiae is 30°C, and the shaker speed is 20...

Embodiment 2

[0050] (1) Pretreatment and sterilization of rapeseed meal raw materials: the rapeseed meal was oven-dried at 45°C for 4 hours, crushed through a 40-mesh sieve, and sterilized by high-pressure steam at 121°C for 20 minutes.

[0051] (2) Preparation of fermented seed liquid: adopt the shake flask culture method to ferment and cultivate Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis respectively, wherein the fermentation medium used by Bacillus amyloliquefaciens and Bacillus subtilis is broth medium, brewing The fermentation medium used by the yeast is potato-glucose medium, both of which are liquid medium; after being sterilized by high pressure steam at 121°C for 20 minutes, it is cooled to room temperature for later use; For several phases, inoculate the test tube liquid into the fermentation medium of the three strains with an inoculum volume of 1% by volume. The culture temperature of Saccharomyces cerevisiae is 28°C, and the shaker speed is 200rp...

Embodiment 3

[0055] (1) Pretreatment and sterilization of rapeseed meal raw materials: the rapeseed meal was oven-dried at 45°C for 4 hours, crushed through a 60-mesh sieve, and sterilized by high-pressure steam at 121°C for 20 minutes.

[0056] (2) Preparation of fermented seed liquid: adopt the shake flask culture method to ferment and cultivate Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis respectively, wherein the fermentation medium used by Bacillus amyloliquefaciens and Bacillus subtilis is broth medium, brewing The fermentation medium used by the yeast is potato-glucose medium, both of which are liquid medium; after being sterilized by high pressure steam at 121°C for 20 minutes, it is cooled to room temperature for later use; For several phases, inoculate the test tube liquid into the fermentation medium of the three strains with an inoculation amount of 1% by volume. The culture temperature of Saccharomyces cerevisiae is 37°C, and the shaker speed is 12...

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Abstract

The invention discloses a probiotics agent for multi-strain combined solid-state fermentation of rapeseed meal and a fermentation method of the probiotics agent. The rapeseed meal is subjected to solid-state fermentation by combining bacillus amyloliquefaciens, saccharomyces cerevisiae and bacillus subtilis to reduce the content of anti-nutritional factors such as glucosinolate. The fermentation method comprises the following steps: pretreating and sterilizing rapeseed meal raw materials, preparing a fermented seed solution, performing solid-state fermentation by probiotics, and preparing a fermented rapeseed meal finished product. According to the probiotics agent disclosed by the invention, the rapeseed meal is subjected to solid-state fermentation by using the bacillus amyloliquefaciensfor the first time, and the bacillus amyloliquefaciens, the saccharomyces cerevisiae and the bacillus subtilis are combined and added into the rapeseed meal for combined fermentation. Compared with other technical schemes, the probiotics agent disclosed by the invention has the advantages that the content of glucosinolate as an anti-nutritional factor in the rapeseed meal can be greatly reduced,meanwhile, the contents of anti-nutritional factors such as tannin, phytic acid and crude fibers in the rapeseed meal can be reduced, the content of peptides in the rapeseed meal finished product is improved, and various bioactive substances can be produced in a probiotics fermentation process. Moreover, the method is low in cost, easy to operate, short in consumed time and high in efficiency.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to a multi-strain combined solid-state fermented rapeseed meal probiotic and a fermentation method thereof. Background technique [0002] Rapeseed meal is a by-product of the production process of rapeseed oil. Generally, the crude protein content is 35% to 40%. The amino acid composition is balanced and contains a large amount of trace elements. It is a commonly used plant protein feed source in the feed industry. Although rapeseed meal is rich in nutrition, the use of rapeseed meal is limited because it contains more anti-nutritional factors, such as glucosinolates, tannins, phytic acid, crude fiber, etc. Glucosinolates not only have certain toxicity, but their main decomposition products such as isothiocyanate and oxazolidinethione are also harmful to livestock and poultry. Combined with metal ions in the feed, it seriously affects the development of the body; tannins not onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20A23K10/37A23K10/18A23K10/12
CPCA23L5/28A23K10/37A23K10/12A23K10/18Y02P60/87
Inventor 朱小燕嵇乐乐李相前李红艳
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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