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A kind of rubber tree chloroplast type hexokinase gene and its encoded protein and application

A technology of rubber leaves and hexokinase, which can be applied in application, genetic engineering, plant genetic improvement and other directions, and can solve problems such as unreported enzyme activity characteristics.

Active Publication Date: 2022-02-22
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

There is no report on Hevea hexokinase (HXK) gene and enzyme activity characteristics

Method used

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  • A kind of rubber tree chloroplast type hexokinase gene and its encoded protein and application
  • A kind of rubber tree chloroplast type hexokinase gene and its encoded protein and application
  • A kind of rubber tree chloroplast type hexokinase gene and its encoded protein and application

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Embodiment Construction

[0029] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0030] 1. Acquisition of Hevea hexokinase coding gene (HbHXK)

[0031]Analyze the hexokinase (HXK) nucleotide sequences of Arabidopsis thaliana, rice, castor and Jatropha curcas that have been registered in NCBI, and screen and assemble a 1800bp rubber tree by searching the Hevea latex transcriptome database we established. After the assembled sequence (contig) of the glycokinase gene, a pair of specific primers were designed to amplify the fu...

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Abstract

The present invention provides a rubber tree chloroplast type hexokinase gene as shown in SEQ ID NO: 1, its encoded protein and its application. The present invention clones the full-length cDNA of Hevea hexokinase HbHXK2 gene for the first time, prokaryotic expression and determination of recombinant protein activity show that the protein encoded by the gene has the function of hexokaryase, the transgenic strain obtained after the gene is transferred into the prokaryotic expression strain The growth rate is significantly increased under normal conditions. Gene expression analysis showed that the HbHXK2 gene was highly expressed in latex, female flowers, and male flowers, and the expression of this gene in latex was the second highest among the genes of the same family; the gene was induced by ethylene and high and low temperature stress at the same time, so the gene may be related to external stress Stimulus-related, it can be used as a target gene to study the prevention and control of abiotic stress stimuli. In addition, the gene can be used as an important genetic resource, and may also be applied in the genetic engineering of other plants or microorganisms other than rubber trees.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a rubber tree chloroplast type hexokinase gene, its encoded protein and its application. Background technique [0002] Hexokinase (hexokinase, HXK, EC 2.7.1.1), as one of the key enzymes in the process of plant respiratory metabolism, can catalyze the phosphorylation of hexose. Recent studies have shown that HXK is also involved in sugar sensing and sugar signal transduction in plants. Hexokinase and plant growth and development [J]. Plant Physiology Communications, 2003, 39(1): 1-8.). Most higher plants synthesize carbohydrates in leaf parts through photosynthesis and distribute them mainly in the form of sucrose to sink tissues via phloem transport. Sucrose in sink tissues can be stored directly or decomposed into hexose by sucrose synthase or invertase (Tang, C.R., Huang, D.B., Yang, J.H., Liu, S.J., Sakr, S., & Li, H.P. .(2010).The sucrose transporter HbSUT3 plays a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/55C12N9/12C12N15/70C12N15/11
CPCC12N9/1205C12Y207/01001C12N15/70C12N15/11
Inventor 龙翔宇方永军房平昌秦云霞阳江华肖小虎
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI