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Chitosan drug-carrying composite microsphere with photo-thermal/redox double response and preparation method thereof

A technology of drug-loaded microspheres and composite microspheres, which is applied in the direction of drug combinations, pharmaceutical formulas, and medical preparations of non-active ingredients. It can solve problems such as small absorption, achieve easy operation, simple preparation process, and improve therapeutic effects. Effect

Active Publication Date: 2020-06-23
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, pure chemotherapy has the problem of multidrug resistance (MDR), and near-infrared (NIR) light can be focused on a specific area and is easy to manipulate, and it can penetrate non-invasively into considerable deep tissue

Method used

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  • Chitosan drug-carrying composite microsphere with photo-thermal/redox double response and preparation method thereof
  • Chitosan drug-carrying composite microsphere with photo-thermal/redox double response and preparation method thereof
  • Chitosan drug-carrying composite microsphere with photo-thermal/redox double response and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of polypyrrole PPy nanoparticles:

[0023] Add 100 mg of sodium lauryl sulfate and 500 mg of pyrrole monomer to 80 mL of distilled water, stir until dissolved and ice bath for 30 minutes. 2mL 0.2mol / L ammonium persulfate solution was added dropwise, and stirred in an ice bath for 3h. After the stirring, the obtained black mixed solution was placed in a dialysis bag (Mw8000-14000), and dialyzed in 1L of water for 48 hours. During this period, the water was changed several times to remove small molecular impurities such as pyrrole that did not participate in the reaction. The black mixed solution obtained by dialysis was freeze-dried at -70° C. for 24 hours to obtain a black solid product as prepared PPy nanoparticles with an average particle size of 20 nm.

[0024] 2) Preparation of chitosan drug-loaded microspheres encapsulating PPy:

[0025] 0.12g chitosan was stirred with 1% HAC and 10mg / mL 5-Fu solution until dissolved, 3mg PPy nanoparticles were ultrasoni...

Embodiment 2

[0029] 1) Prepare polypyrrole PPy nanoparticles according to the same steps as in Example 1:

[0030] 2) Preparation of chitosan drug-loaded microspheres encapsulating PPy:

[0031] 0.3g chitosan was stirred with 1% HAC and 10mg / mL 5-Fu solution until dissolved, 8mg PPy nanoparticles were ultrasonically dispersed in the chitosan solution, 2 drops of Tween-80 were added, and ultrasonic emulsification was performed for 3 minutes. 150μL of glutaraldehyde was added to 24mL of liquid paraffin, then 3 drops of Span-80 were added, and the emulsion was made by ultrasonic emulsification for 3 minutes. Then, the emulsion was added dropwise to the chitosan solution and stirred for 40 minutes, and then the temperature was raised to 40°C, and finally 50 μL of glutaraldehyde was dropped into the chitosan drug-loaded microspheres for 2 hours to obtain PPy-encapsulated chitosan.

[0032] 3) Preparation of chitosan / carboxymethylcellulose composite drug-loaded microspheres encapsulating PPy:

[0033] ...

Embodiment 3

[0035] 1) Prepare polypyrrole PPy nanoparticles according to the same steps as in Example 1:

[0036] 2) Preparation of chitosan drug-loaded microspheres encapsulating PPy:

[0037] 0.2g chitosan was stirred with 1% HAC and 10mg / mL 5-Fu solution until dissolved, 1mg PPy nanoparticles were ultrasonically dispersed in the chitosan solution, 1 drop of Tween-80 was added, and ultrasonic emulsification was performed for 3 minutes. 75μL of glutaraldehyde was added to 24mL of liquid paraffin, then 2 drops of Span-80 were added, and the emulsion was made by ultrasonic emulsification for 3 minutes. Then, the emulsion was added dropwise to the chitosan solution and stirred for 40 minutes, and then the temperature was raised to 40°C, and finally 30 μL of glutaraldehyde was dropped into the solution for 2 hours to obtain the chitosan drug-loaded microspheres encapsulating PPy.

[0038] 3) Preparation of chitosan / carboxymethylcellulose composite drug-loaded microspheres encapsulating PPy:

[0039...

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Abstract

The invention discloses a chitosan drug-carrying composite microsphere with photo-thermal / redox double response and a preparation method thereof. The preparation method comprises the following steps:firstly, wrapping polypyrrole nanoparticles and an anti-tumor drug 5-fluorouracil with chitosan to form nano microspheres; secondly, adsorbing carboxymethyl cellulose on an outer layer through electrostatic action; and finally, performing crosslinking by using a disulfide compound to form the composite drug-carrying microspheres. After the drug-carrying system provided by the invention enters intoblood circulation, under the stimulation of high-concentration glutathione in tumor tissues, the drug-carrying system releases anticancer drugs by reductive response, and exerts an anticancer effect.Meanwhile, the prepared nano drug delivery system not only has a thermotherapy effect under near infrared photothermal irradiation, but also can control thermal response of the drugs so as to achievethe purpose of combined therapy.

Description

Technical field [0001] The invention belongs to the technical field of drug carrier preparation, and relates to a chitosan composite drug delivery system corresponding to photothermal and redox. Specifically, it relates to a preparation method of polypyrrole nano particles encapsulating near-infrared light response and chitosan composite drug-loaded microspheres cross-linked by disulfide bonds. Background technique [0002] Although many researchers are actively solving cancer treatment problems, they still face huge challenges due to the complexity and diversity of tumor occurrence and progression. Chemotherapy, as one of the most common treatment methods in clinical application, has made great progress in inhibiting tumor proliferation and prolonging the lives of patients. However, traditional chemotherapy drugs are often limited by the specificity and selectivity of the tumor site, which may cause systemic toxicity and serious adverse reactions. Nanocarrier-mediated responsi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/61A61K47/69A61K9/50A61K47/34A61K45/06A61K47/36A61P35/00
CPCA61K41/0052A61K47/61A61K47/6927A61P35/00A61K9/5031A61K47/36A61K41/0042A61K9/0009A61K45/06
Inventor 王芳李佳芮胡胜黄可欣
Owner NANJING FORESTRY UNIV
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