Method for improving sensitivity of nicotiana tabacum to cadmium

Abstract

The invention relates to a method for improving sensitivity of nicotiana tabacum to cadmium, and in particular relates to the method of overexpressing MhNRAMP6 in the nicotiana tabacum by using a leafdisc infiltration process. The length of the open reading frame (ORF) of the MhNRAMP6 gene is 1638 bp, a protein containing 545 amino acids is encoded, the protein molecular weight is predicted to be59.12 kD, and the MhNRAMP6 is located in a plasma membrane, and has 11 strong transmembrane helices; and the nucleotide sequence is shown as SEQ No.1. The nicotiana tabacum containing the overexpression gene MhNRAMP6 is more sensitive to the cadmium, so that the tolerance of the nicotiana tabacum to the cadmium is reduced, the problem that the nicotiana tabacum generates various stress reactionsbecause heavy metal Cd pollution in tobacco growing areas is increased is avoided, and the absorption of the cadmium by nicotiana tabacum roots is increased to enrich environmental cadmium to achievethe purpose of reducing the concentration of cadmium in the external environment.

Description

technical field [0001] The invention relates to a method for improving tobacco cadmium sensitivity, which belongs to the research field of the influence of cadmium on tobacco growth. Background technique [0002] Cadmium (Cd) is a heavy metal with strong toxicity, which can inhibit plant growth at a low concentration. Long-term absorption and accumulation will promote plant cells to produce a large amount of active oxygen, promote protein denaturation and inactivation, and then damage plant cell membranes, cause damage to the internal structure of cells, disrupt cell metabolism balance, cause spots on leaves, reduce photosynthetic efficiency, and cause slow plant growth , short plants, and even cause plant disease and death, ultimately affecting crop yield and quality (Chen Aikui et al., 2013; Ma Yanhua et al., 2014). With the development of industrialization, the pollution of water sources and the discharge of waste gas have caused the cadmium content in some soils used fo...

AI Technical Summary

Problems solved by technology

Long-term absorption and accumulation will promote plant cells to produce a large amount of active oxygen, promote protein denaturation and inactivation, and then damage plant cell membranes, cause damage to the internal structure of cells, disrupt cell metabolism balance, cause spots on leaves, reduce photosynthetic efficiency, and cause slow plant growth , short plants, and even cause plant disease and death, ultimately affecting crop yield and quality (Chen Aikui et al., 2013; Ma Yanhua et al., 2014)

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