A kind of method for subcritical water extraction and separation of Ganoderma lucidum triterpenoid extract

A technology of Ganoderma lucidum triterpenes and subcritical water, which is applied in the field of subcritical water extraction and separation of Ganoderma lucidum triterpenoid active extracts, can solve the problem that it is difficult to truly reflect the extraction rate of Ganoderma lucidum triterpenoid components in subcritical extraction, and the unspecified Ganoderma lucidum triterpenoid acids are not specified. Purity and pharmacological activity, unexplained Ganoderma lucidum polysaccharide purity and pharmacological activity, etc., to achieve good antioxidant activity, inhibit tumor cell growth activity, and low cost

Active Publication Date: 2022-07-05
福建仙芝楼生物科技有限公司 +1
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Problems solved by technology

Deng Chenchen et al. ("Study on the technology of extracting Ganoderma lucidum polysaccharides with subcritical water", Deng Chenchen et al., Journal of Henan University of Technology (Natural Science Edition), 2016, 37(2): 105-108) although the conditions for extracting Ganoderma lucidum polysaccharides with subcritical water To optimize, but still need to go through the steps of ultrafiltration, protein removal, and alcohol precipitation to obtain Ganoderma lucidum polysaccharides. The steps are cumbersome, and the purity and pharmacological activity of Ganoderma lucidum polysaccharides obtained are not explained.
[0007] Xu Ruiling et al. ("Optimization of subcritical water extraction of ganoderma acid components in ganoderma lucidum fruiting bodies", Xu Ruiling et al., West China Pharmaceutical Journal, 2019, 34(4): 393~395) on subcritical extraction of ganoderma acid (i.e., the acidic part of the triterpenoids of Ganoderma lucidum) components are optimized, but the liquid phase detection conditions use Ganoderma acid A and Ganoderma acid C2 as reference substances, and only detect the total content of Ganoderma acid A and Ganoderma acid C2, because they are in the Ganoderma acid The proportion of the acidic part of Ganoderma lucidum is too small, it is not easy to reflect the complexity of Ganoderma lucidum acid; since the neutral part of Ganoderma lucidum triterpenes is not detected, it is difficult to truly reflect the extraction rate of subcritical extraction of Ganoderma lucidum triterpenoids
At the same time, the purity and pharmacological activity of the ganoderma triterpene acid obtained by it are not stated.

Method used

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  • A kind of method for subcritical water extraction and separation of Ganoderma lucidum triterpenoid extract
  • A kind of method for subcritical water extraction and separation of Ganoderma lucidum triterpenoid extract
  • A kind of method for subcritical water extraction and separation of Ganoderma lucidum triterpenoid extract

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Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0036] 1. Pulverize the fruiting body of Ganoderma lucidum, and take 1kg of Ganoderma lucidum granules of 40-60 mesh;

[0037] 2. Add 10kg of water by weight to Ganoderma lucidum granules, extract for 10min under a pressure of 5Mpa and a temperature of 170°C, filter, add 10kg of water to rinse, and combine to obtain filtrate I; the relative density of filtrate I at 25°C is 1.00-1.10 after concentration. , add 95% ethanol alcohol precipitation, the final concentration of alcohol precipitation is 70%, centrifuge, the separation factor is 6000~8000 during centrifugation, the turbidity of the supernatant is below 100NTU, the supernatant is concentrated and dried to obtain the acid part of Ganoderma lucidum triterpene (sample 1); Ganoderma lucidum triterpene acid part content reaches 29.2%. Ganoderma lucidum polysaccharide fraction (sample 5) was obtained after the precipitate was dried;

[0038] 3. Add 10kg of water to the Ganoderma lucidum particles extracted in step 2, extract ...

specific Embodiment 2

[0039] 1. Pulverize the fruiting body of Ganoderma lucidum, and take 1kg of Ganoderma lucidum granules of 40-60 mesh;

[0040] 2. Add 10kg weight of Ganoderma lucidum particles, extract 10min under a pressure of 5Mpa and a temperature of 180°C, filter, add 10kg of water to rinse, and combine to obtain filtrate I; the relative density of filtrate I after concentration is 1.00~1.10 (25°C) , add 95% ethanol alcohol precipitation, the final concentration of alcohol precipitation is 70%, centrifuge, the separation factor is 6000~8000 during centrifugation, the turbidity of the supernatant is below 100NTU, the supernatant is concentrated and dried to obtain the acid part of Ganoderma lucidum triterpene (sample 2), the content of Ganoderma lucidum triterpene acid part reaches 28.2%; the Ganoderma lucidum polysaccharide part (sample 6) is obtained after precipitation and drying;

[0041] 3. Add 10kg of water to the Ganoderma lucidum particles extracted in step 2, extract at a pressure...

specific Embodiment 3

[0042] 1. Pulverize the fruiting body of Ganoderma lucidum, and take 1kg of Ganoderma lucidum granules of 40-60 mesh;

[0043] 2. Add 10kg weight of Ganoderma lucidum particles, extract 10min under a pressure of 5Mpa and a temperature of 190°C, filter, add 10kg of water to rinse, and combine to obtain filtrate I; the relative density of filtrate I after concentration is 1.00~1.10 (25°C) , add 95% ethanol alcohol precipitation, the final concentration of alcohol precipitation is 70%, centrifuge, the separation factor is 6000~8000 during centrifugation, the turbidity of the supernatant is below 100NTU, the supernatant is concentrated and dried to obtain the acid part of Ganoderma lucidum triterpene (sample 3), the triterpene acid part content of Ganoderma lucidum reaches 25.5%; the Ganoderma lucidum polysaccharide part (sample 7) is obtained after precipitation and drying;

[0044] 3. Add 10kg of water to the Ganoderma lucidum particles extracted in step 2, extract at a pressure...

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Abstract

The invention provides a method for extracting and separating Ganoderma lucidum triterpenoid extract by using subcritical technology: subjecting Ganoderma lucidum to subcritical water extraction technology, using different pressure and temperature conditions, and sequentially extracting the active components in Ganoderma lucidum: the acidic part of Ganoderma lucidum triterpene and the Ganoderma lucidum triterpenoid neutral parts were extracted and isolated from Ganoderma lucidum fruiting bodies. The invention utilizes subcritical water extraction technology to obtain different active extracts in Ganoderma lucidum efficiently and economically. The antioxidant activity of Ganoderma lucidum triterpenoid acid site DPPH clearance rate in the obtained active extract can reach 88.38%, and the half-inhibition rate IC of Ganoderma lucidum triterpenoid neutral site on human large cell lung cancer cell H460 50 95.0μg / ml, the half-inhibition rate IC of human hepatoma cell HepG2 50 was 77.7 μg / ml.

Description

technical field [0001] The invention belongs to the fields of natural product extraction and edible and medicinal fungi, relates to the technical field of processing and utilization of Ganoderma lucidum, and in particular relates to a method for subcritical water extraction and separation of Ganoderma lucidum triterpenoid active extract Background technique [0002] Ganoderma lucidum (Leyss.ex Fr.) Karst.) is a kind of Ganoderma lucidum (Chinese Pharmacopoeia, 2015 edition), which is a kind of fungus Ganoderma lucidum (Chinese Pharmacopoeia 2015 edition). , Ganoderma lucidum triterpenoids have anti-oxidation, enhance immunity, anti-tumor, liver and other pharmacological effects. Ganoderma lucidum triterpenes are divided into acidic parts and neutral parts, among which the acidic parts contain pentacyclic triterpenoids such as Ganoderma A, which have been reported in a large number of literatures. The neutral part contains sterols such as Ganoderma ketonetriol, which are les...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/074A61P39/06A61P35/00
CPCA61K36/074A61P39/06A61P35/00A61K2236/331A61K2236/37A61K2236/55A61K2236/53Y02P20/54
Inventor 周岩飞李晔姚渭溪朱忠敏林真陈桂秋吴长辉许建华郑光耀
Owner 福建仙芝楼生物科技有限公司
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