Liquid sample vortex differential centrifugal extraction device and extraction method
A liquid sample and extraction device technology, applied in liquid solution solvent extraction, measuring device, material separation, etc., can solve the problems of complicated operation and long extraction time, and achieve the effect of expanding the contact area, speeding up the extraction balance, and simple and fast application.
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Embodiment 1
[0032] The vortex extraction process of the vortex differential centrifugal extraction device can refer to the attached figure 2 . In this example, graphene oxide (GO) was selected as the extractant, as a honeycomb-shaped two-dimensional planar film composed of a single layer of carbon atoms, and its chemical composition is mainly composed of carbon atoms and polar oxygen-containing functional groups; The hydroxyl and epoxy groups on the surface, and the carboxyl groups on the edge make it extremely hydrophilic. Polyvinylidene fluoride microporous filter membrane (PVDF) was used as the support layer, polydopamine was used as the cross-linking agent, and GO and PVDF were cross-linked according to the conventional cross-linking reaction operation. The method for extracting with an extraction device comprises the following steps:
[0033] Adsorption process: roll the extraction film into a tube and place it in the sample tube, add the liquid sample to be tested, use a vortex m...
Embodiment 2
[0034] Embodiment 2, the rapid analysis of ephedrine in urine
[0035] Carry out rapid extraction analysis for the ephedrine in urine, extraction device is the same as embodiment 1, and its extraction process can refer to attached image 3 conduct. Adsorption process: roll the extraction membrane into a tube and place it in a 10 mL centrifuge tube, add 10 mL of the spiked urine sample solution, vortex for 5 min, and discard the sample solution; matrix removal process: wash the extraction membrane with 5 mL of ultrapure water and a centrifuge tube, discard the washing water, and repeat 3 times; elution process: Add 1 mL of methanol, vortex elution for 10 min, and analyze the eluate by ion mobility spectrometry.
[0036] Blank urine and ephedrine spiked urine were used as controls, and were extracted by vortex centrifugation and analyzed by ion mobility spectrometry of ephedrine respectively. The results are shown in the attached Figure 4 shown. After being treated by the vo...
Embodiment 3
[0037] Embodiment 3, the rapid analysis of ephedrine in saliva
[0038] Take 2 mL of saliva in the vortex differential centrifugation device of Example 1, add 5 mL of phosphate buffered saline (pH 7.2-7.4) to dilute the saliva sample, seal it with a stopper, vortex centrifuge for 10 min, and discard the liquid in the sample tube , followed by phosphate buffered saline solution and ultrapure water to remove residual impurities in the sample, the extraction membrane was eluted with 0.5 mL of methanol, and the eluate was analyzed by ion mobility spectrometry.
[0039] Blank saliva and ephedrine-spiked saliva were used as controls, which were extracted by vortex centrifugation and then analyzed by ion mobility spectrometry. The result is as Figure 5 shown by Figure 5 It can be seen that the mucin, mucopolysaccharide, salivary amylase, lysozyme, immunoglobulin, inorganic salt and other matrices in saliva were removed, and there was no interference in IMS analysis, and the whole...
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