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Production method of steviol glycoside

A technology of stevioside and production method, which is applied in the field of production of stevioside, can solve the problems of high loss rate of stevioside, achieve the effects of low loss rate, efficient removal of impurities, and increase of yield

Pending Publication Date: 2020-06-30
BENGBU HUADONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional chemical flocculants such as iron and calcium compounds have good impurity removal effects, due to the introduction of inorganic ions, it is necessary to increase the process of removing inorganic ions, and the colloid formed during the flocculation process will absorb steviol glycosides, and the loss rate of steviol glycosides Big

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A production method of steviol glycosides, comprising the following steps:

[0026] S1. Grinding the dried stevia leaves to 20 mesh to obtain stevia powder;

[0027] S2. Add stevia powder into water equivalent to 10 times the mass of the stevia powder, extract at 40°C, and filter; repeat the above steps 4 times, and combine the filtrates obtained each time to obtain stevia water extract ;

[0028] S3. After adding the acetic acid solution of chitosan into the stevia aqueous extract and mixing evenly, adjust the pH to 3, flocculate at 30° C. for 2 hours, filter after flocculation, and dry the obtained filtrate to obtain a crude stevia extract. thing;

[0029] S4, carry out supercritical CO to described stevia rebaudiana crude extract 2 Extraction, the extraction conditions are: use ethanol solution with a concentration of 10% as entrainer, at a temperature of 55°C, a pressure of 30MPa, CO 2 Extract for 1.5 h under the condition of a flow rate of 15 L / h, and dry the o...

Embodiment 2

[0034] A production method of steviol glycosides, comprising the following steps:

[0035] S1. Grinding the dried stevia leaves to 100 mesh to obtain stevia powder;

[0036] S2. Add stevia powder into water equivalent to 15 times the mass of the stevia powder, extract at 60°C, and filter; repeat the above steps twice, and combine the filtrates obtained each time to obtain stevia water extract ;

[0037] S3. After adding the acetic acid solution of chitosan into the stevia aqueous extract and mixing evenly, adjust the pH to 5, flocculate at 50° C. for 5 hours, filter after flocculation, and dry the obtained filtrate to obtain a crude stevia extract. thing;

[0038] S4, carry out supercritical CO to described stevia rebaudiana crude extract 2 Extraction, the extraction conditions are: use ethanol solution with a concentration of 30% as entrainer, at a temperature of 75°C, a pressure of 35MPa, CO 2 Extract for 1 hour under the condition of a flow rate of 25 L / h, and dry the o...

Embodiment 3

[0043] A production method of steviol glycosides, comprising the following steps:

[0044] S1. Grinding the dried stevia leaves to 60 mesh to obtain stevia powder;

[0045] S2. Add stevia powder to water equivalent to 12 times the mass of the stevia powder, extract at 50°C, and filter; repeat the above steps 3 times, and combine the filtrates obtained each time to obtain stevia water extract ;

[0046] S3. After adding the acetic acid solution of chitosan into the stevia aqueous extract and mixing evenly, adjust the pH to 3.5, flocculate at 40° C. for 3 hours, filter after flocculation, and dry the obtained filtrate to obtain a crude stevia extract. thing;

[0047] S4, carry out supercritical CO to described stevia rebaudiana crude extract 2 Extraction, the extraction conditions are: use ethanol solution with a concentration of 20% as entrainer, at a temperature of 60°C, a pressure of 32MPa, CO 2 Extracting for 1.25h under the condition of a flow rate of 18L / h, drying the ...

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Abstract

The invention discloses a production method of steviol glycoside. The production method comprises the following steps of S1, crushing dried sugar stevia leaves to obtain sugar stevia leaf powder; S2,leaching the sugar stevia leaf powder with water, and performing filtering to obtain an sugar stevia leaf aqueous extract solution; S3, adding an acetic acid solution of chitosan to the sugar stevia leaf aqueous extract solution, performing uniform mixing, performing flocculating, after flocculating, performing filtering, and drying obtained filtrate to obtain a sugar stevia leaf crude extract; S4, performing supercritical CO2 extraction on the sugar stevia leaf crude extract to obtain extraction liquid; S5, purifying the sugar stevia leaf extract with macroporous adsorptive resins, collectingeluents, and performing concentrating to obtain concentrated liquid; and S6, performing decoloring on the concentrated liquid, and performing drying to obtain the steviol glycoside. According to thepreparation method, the loss rate of the steviol glycoside in the process of removing impurities can be reduced, impurities are efficiently removed, and the method has the advantages of being high inyield and high in purity.

Description

technical field [0001] The invention relates to the technical field of stevioside production, in particular to a production method of stevioside. Background technique [0002] Steviol glycoside is a low-calorie, high-fold natural sweetener extracted from stevia leaves. Its sweetness is 200-300 times that of sucrose, and its calories are only about 1 / 250 of sucrose. , heart disease, etc. have a certain auxiliary therapeutic effect, and have no toxic and side effects, and can be used as a sucrose substitute. It is a functional sweetener with great development value. [0003] At present, the extraction methods of steviol glycosides mainly include hot water extraction method, decoction method, reflux method, etc. The stevia extract obtained contains a large amount of impurities such as protein, organic acid, saponin, etc., so it must be purified. The usual purification method is to use flocculants to remove impurities from the stevia extract, then perform desalination, decolori...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
CPCC07H15/256C07H1/08Y02P20/54
Inventor 邢飞邢伟张志华庞正银
Owner BENGBU HUADONG BIOLOGICAL TECH
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