Extraction method of soapnut saponin

Abstract

The invention discloses an extraction method of soapnut saponin, which comprises: S1, taking sapindus peel, carrying out water leaching for a plurality of times, respectively collecting all the leaching solutions and leaching residues, and removing the part with the molecular weight of more than 1000 from the leaching solutions to obtain a water leaching clear solution; extracting leaching residues with alcohol, combining extracting solutions, and concentrating to obtain a concentrated clear solution; S2, combining the water leaching clear liquid with the concentrated clear solution, purifyingan obtained solution through non-polar macroporous resin, and eluting by using pure water and three sections of ethanol water solutions with different concentrations in sequence; S3, collecting and concentrating a second-stage eluent and a third-stage eluent, and drying obtained concentrated solutions respectively to obtain a powder I and a powder II, wherein the powder I is echinocystic acid type saponin, and the powder II is oleanolic acid type saponin. The soapnut saponin product obtained by adopting the elution gradient of the scheme is high in purity, high in yield and light in color, isoff-white, does not need to use toxic reagents, and is environment-friendly.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting Sapindus saponins. Background technique [0002] Sapindus belongs to Sapindus family Sapindus, scientific name: Sapindus Mukurossi Gaertn, Compendium of Materia Medica is called Mukurossi Gaertn, Sichuan is called Oil Sapindus, Hainan Island is called Sapindus Mukurossi Gaertn, and Taiwan is called Sapindus Mukurossi Gaertn. It is Huangmuzi, also known as oil luoshu, washing fruit, soap fruit tree and so on. Sapindus saponin is the extract of Sapindus pericarp, and its main active ingredients are triterpene saponins, sepinene glycosides, fatty oil and protein. According to different aglycones, triterpene saponins can be divided into oleanolic acid saponins and echinocystic acid saponins, etc. According to research reports, oleanolic acid saponins are effective in antitumor, hypoglycemic, hypolipidemic, anti-inflammatory and antibacterial, and ant...

AI Technical Summary

Problems solved by technology

The final product of this purification method has higher purity and low cost, but it still has the following disadvantages: 1. there are many factors affected, including the pH value of the solution, temperature, etc.; 2. the process is relatively complicated, and it is difficult to realize industrial production, and Use a large amount of organic reagents, which is harmful to the environment; ③This process cannot obtain two different types of Sapindus at one time

Chinese invention patent CN104292294A discloses a method for the purification of sapindus sapinin, specifically disclosing that two different types of sapindus saponins are obtained by extraction method: extracting sapindus pericarp into absolute ethanol, centrifuging Add acetone to the supernatant and carry out sedimentation centrifugation, add ether to the supernatant after centrifugation and carry out sedimentation centrifugation to obtain two different types of Sapindus saponins. However, this method has the following disadvantages: ① But this process uses A large number of organic reagents cause great pollution to the environment and are unsafe

② Not suitable for mass industrial production