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High-density fermentation method of saccharomycetes

A technology for yeast and fermentation medium, which is applied in the field of high-density fermentation of yeast, can solve the problems of low density of yeast cells at the end of fermentation, long fermentation time, and unfavorable reproduction of Candida utilis cells, and the like. It is beneficial to reproduction, increase the density of bacteria, and increase the speed of growth and reproduction.

Active Publication Date: 2020-06-30
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the existing method for fermenting Candida utilis is mainly to inoculate Candida utilis in the fermentation medium, and cultivate it at 30-35° C. for about 30 hours at a rotating speed of 200-600 rpm. However, carrying out the fermentation and cultivation of Candida utilis according to the existing method is not conducive to the reproduction of Candida utilis thallus, resulting in low density of yeast thallus at the end of fermentation and longer fermentation time

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  • High-density fermentation method of saccharomycetes

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Embodiment approach

[0030] According to the present invention, the fermentation medium of the yeast can be a conventional medium for yeast fermentation in the art, for example, it can be a conventional YEPD medium, and according to a preferred embodiment of the present invention, the The fermentation medium contains glucose, potassium, magnesium and ammonium salts.

[0031] According to the present invention, the potassium salt can be various potassium salts conventionally used in the fermentation field, such as, but not limited to, potassium chloride, potassium sulfate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, etc., preferably , the potassium salt is dipotassium hydrogen phosphate.

[0032] According to the present invention, the magnesium salt can be various magnesium salts commonly used in the fermentation field, for example, but not limited to, magnesium chloride, magnesium sulfate, etc., preferably, the magnesium salt is magnesium sulfate.

[0033] According to the pr...

preparation example

[0050] This example is used to illustrate the preparation of yeast seed liquid

[0051] (1) The Candida utilis stored in an ultra-low temperature refrigerator was thawed naturally 0.5 h in advance, and transferred to an activated primary seed shake flask according to the inoculum amount of 0.1% by weight. Cultivate in a reciprocating shake flask cabinet at 30-32°C and a rotation speed of 120rpm. When the OD600 grows to 0.20-0.25, the cultivation is stopped, and the yeast seed liquid is obtained.

[0052] Seed culture medium: 0.5% by weight of yeast powder, 1% by weight of peptone, 0.5% by weight of glucose, and the pH value is 6.0-6.2.

Embodiment 1

[0054] This embodiment is used to illustrate the high-density fermentation method of the yeast provided by the invention

[0055] (1) Glucose, dipotassium hydrogen phosphate, magnesium sulfate and ammonium sulfate are mixed, and an appropriate amount of deionized water is added, wherein, the addition amount of each component makes in the obtained fermentation medium, the content of glucose is 2.0% by weight, The content of dipotassium hydrogen phosphate was 0.3% by weight, the content of magnesium sulfate was 2.0% by weight, and the content of ammonium sulfate was 0.8% by weight. The pH value of the obtained fermentation medium was adjusted to 6.0-6.2, sterilized at 121° C. for 20 minutes, and cooled to room temperature for use.

[0056] (2) Inoculate the seed solution obtained in the preparation example into the fermentation medium prepared in step (1) with an inoculation amount of 0.15% by weight, and cultivate at 32-33° C. at a rotating speed of 400 rpm, and continuously ta...

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Abstract

The invention relates to the field of fermentation of saccharomycetes, in particular to a high-density fermentation method of saccharomycetes. The method comprises the following steps: inoculating saccharomycetes into a fermentation medium for fermentation, and starting to add a nutritional supplement into the fermentation medium in the logarithmic growth phase of the saccharomycetes fermentation,wherein the nutritional supplement contains yeast powder, glucose, potassium salt, magnesium salt and ammonium salt. According to the invention, in the logarithmic growth phase of the saccharomycetesfermentation, the nutritional supplement containing the yeast powder, the glucose, the potassium salt, the magnesium salt and the ammonium salt is added into fermentation liquor, and the shortage andimbalance of nutrient substances in the growth process of the saccharomycetes can be effectively overcome, so that the fermentation condition is beneficial to propagation of saccharomycetes thalli, the thallus density in the fermentation liquor is increased, and the dry weight of the fermented thalli is increased; and meanwhile, due to the increase of the growth and propagation speed of the saccharomycetes, the fermentation period is also remarkably shortened.

Description

technical field [0001] The invention relates to the field of fermentation of yeast, in particular to a high-density fermentation method of yeast. Background technique [0002] Candida utilis is a very good single-cell protein feed. It contains high crude protein, amino acid and multivitamins, and also contains a variety of digestive enzymes (amylase, protease, cellulase, etc.) required by animals, which play an important role in the digestion and utilization of nutrients. [0003] However, the existing method for fermenting Candida utilis is mainly to inoculate Candida utilis in the fermentation medium, and cultivate it at 30-35° C. for about 30 hours at a rotating speed of 200-600 rpm. However, carrying out the fermentation and cultivation of Candida utilis according to the existing method is unfavorable for the reproduction of Candida utilis cells, resulting in a low density of yeast cells at the end of fermentation and a long fermentation time. Contents of the inventio...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12R1/72
CPCC12N1/16
Inventor 佟毅卢宗梅王梅陈影李义邴狄祥卜庆阳
Owner COFCO BIOTECHNOLOGY CO LTD