Brevibacillus laterosporus and composition thereof and application of Brevibacillus laterosporus and composition

A technology of Bacillus brevius and antibacterial composition, applied in the field of biological feed additives, can solve problems such as environmental pollution, destruction of intestinal microorganisms, and drug-resistant bacteria, and achieve the effects of environmental friendliness, broad application prospects, and safety for humans and animals

Active Publication Date: 2020-07-10
中粮生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The harm of misuse of antibiotics is extremely serious. It will not only lead to the emergence of drug-resistant bacteria, but also cause various problems such as the decline in

Method used

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  • Brevibacillus laterosporus and composition thereof and application of Brevibacillus laterosporus and composition
  • Brevibacillus laterosporus and composition thereof and application of Brevibacillus laterosporus and composition
  • Brevibacillus laterosporus and composition thereof and application of Brevibacillus laterosporus and composition

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0043] Example 1: Isolation and screening of the starting strains of Brevibacterium lateralis

[0044] According to the document "Isolation and structural elucidation of brevibacillin, anantimicrobial lipopeptide from Brevibacillus laterosporus that combats drug-resistant gram-positive bacteria", Applied and environmental microbiology (2016), one strain was isolated and screened from the soil of Fuhai County, Xinjiang. Staphylococcus aureus ATCC26001 is a wild-type Brevibacterium lateralis (original strain) with good inhibitory effect.

[0045] Perform 16S rDNA identification and identification of physiological and biochemical characteristics of the obtained strain (refer to the "Berger Bacterial Identification Manual", Science Press, 1984 for identification), determine that it is Brevibacterium lateralis, and use it as a starting point Strains were subjected to subsequent experiments.

Example Embodiment

[0046] Example 2: Mutagenesis of wild-type Brevibacterium lateralis and screening of mutant strains

[0047] Using atmospheric pressure room temperature plasma (ARTP) mutagenesis technology to mutagenize the wild-type Bacillus lateralis obtained in Example 1, and then through high-throughput screening to obtain an obvious inhibitory effect on Staphylococcus aureus ATCC26001 Brevibacterium lateralis B8. Specific steps are as follows:

[0048] 1) Pick out a single colony of the hair strain and inoculate it into fresh LB liquid medium, culture at 37°C for 24h, transfer to fresh LB liquid medium, and cultivate to the OD of the bacterial solution 600 Is 2-3;

[0049] 2) Use sterile water to dilute the above bacterial solution to OD 600 For 1, draw 10μL of the diluted bacterial solution and evenly spread it on the slide, use the atmospheric pressure room temperature plasma (ARTP) mutagenesis breeder for mutagenesis, place the slide under the treatment source, and induce the treatment for ...

Example Embodiment

[0054] Example 3: Cultivation of Brevibacterium Laterosporus B8 and determination of antibacterial activity

[0055] 3-1 Dry spray powder of culture of Brevibacterium Laterosporus B8 and its antibacterial activity

[0056] The following steps were used to prepare the culture of Brevibacterium lateralis B8 obtained in Example 2 into a spray powder:

[0057] 1) Transform the strain of Brevibacterium lateralis B8 into fresh LB medium at an inoculum of 0.2v / v%, cultivate overnight at 37°C, and then insert the resulting culture solution into the above-mentioned medium at 1v / v% In Brevibacterium lateralis culture medium, culture at 200 rpm at 37°C for three days to obtain fermentation broth; take out 1 mL of fermentation broth and centrifuge at room temperature (8000 rpm, 10 min) to obtain fermentation broth supernatant

[0058] 2) Boil the remaining fermentation broth at a temperature of 100°C for 15 minutes, and spray dry to obtain spray dry powder (set spray drying conditions: inlet temp...

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Abstract

The invention discloses a strain of brevibacillus laterosporus B8 and a composition comprising the same and an application of the brevibacillus laterosporus B8 and the composition. The brevibacillus laterosporus B8 disclosed by the invention has a potent inhibitory effect on animal pathogens, such as staphylococcus aureus, can secrete antibacterial peptides, can be made into an antibacterial agentand has a good prevention and treatment effect on gram-negative bacteria and gram-positive bacteria, so as to replace antibiotics to play an antibacterial role in a feed and reduce use of the antibiotics in the biological feed field; and furthermore, the brevibacillus laterosporus B8 disclosed by the invention does not have toxicity or pathogenicity, is safe for people and animals and friendly toenvironment, is a useful resource for research and development of a biological feed and has a potential application prospect.

Description

technical field [0001] The invention relates to the field of biological feed additives, in particular, the invention relates to a Brevibacillus laterosporus (Brevibacillus laterosporus) with antibacterial effect, an antibacterial composition comprising the same and uses thereof. Background technique [0002] As one of the greatest inventions in the medical field in the 20th century, antibiotics have played an extremely important role in the prevention and treatment of various diseases. As a major producer of antibiotics, China uses antibiotics particularly frequently. It is estimated that the annual production of antibiotic raw materials in my country is about 210,000 tons, of which about 97,000 tons are used in animal husbandry. Since it was discovered in the 1950s that the use of low-concentration antibiotics in feed can not only prevent diseases, but also promote the growth of livestock and poultry, various antibiotics have been widely added to feed, and the abuse of ant...

Claims

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Application Information

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IPC IPC(8): C12N15/20A01N63/20A01P1/00C12R1/13
CPCC12R2001/13C12N1/205
Inventor 杨鑫朱峰华安泰陈博王靖臧传刚朱威宇焦琳郑晓卫金渭武
Owner 中粮生物科技(北京)有限公司
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