Preparation method and application of oral microspheres loaded with MSCs-derived exosomes

A technology of exosomes and sources, applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, microcapsules, etc., can solve the problem of large infusion dose, low efficiency of autoimmune diseases, and ineffective treatment. Stability and other issues to achieve the effect of slowing down the deactivation

Active Publication Date: 2020-07-17
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the shortcomings of MSCs exosomes in the treatment of autoimmune diseases, such as low efficiency, large infusion dose, and unstable therapeutic effect, the present invention provides a preparation of oral microspheres loaded with MSCs-derived exosomes and its application in the treatment of RA. Applications

Method used

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  • Preparation method and application of oral microspheres loaded with MSCs-derived exosomes
  • Preparation method and application of oral microspheres loaded with MSCs-derived exosomes
  • Preparation method and application of oral microspheres loaded with MSCs-derived exosomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] An oral microsphere loaded with exosomes derived from MSCs, the preparation method of which comprises the following steps:

[0038] (1) Preparation of ALG / CST outer core solution:

[0039] Weigh sodium alginate ALG and chitosan CST respectively, add each into double-distilled water to disperse, prepare sodium alginate solution and chitosan solution, and fully dissolve them respectively; after cooling to room temperature, mix the two according to a certain ratio, and stir thoroughly until uniform; the concentration of the ALG / CST outer core solution is 1% ALG / 1% CST.

[0040] (2) Preparation of ALG / CNC core solution:

[0041] Weigh sodium alginate ALG and sodium hydroxymethylcellulose CNC-Na respectively, add each into double-distilled water to disperse, and dissolve fully respectively to prepare sodium alginate solution and sodium hydroxymethylcellulose solution; after cooling to room temperature, the two or mixed according to a certain ratio, fully stirred until unifor...

Embodiment 2

[0048] An oral microsphere loaded with exosomes derived from MSCs, the preparation method of which comprises the following steps:

[0049] (1) Preparation of ALG / CST outer core solution:

[0050] Weigh sodium alginate ALG and chitosan CST respectively, add each into double-distilled water to disperse, prepare sodium alginate solution and chitosan solution, and fully dissolve them respectively; after cooling to room temperature, mix the two according to a certain ratio, and stir thoroughly until uniform; the concentration of the ALG / CST outer core solution is 1% ALG / 1% CST.

[0051] (2) Preparation of ALG / CNC core solution:

[0052] Weigh sodium alginate ALG and sodium hydroxymethylcellulose CNC-Na respectively, add each into double-distilled water to disperse, and dissolve fully respectively to prepare sodium alginate solution and sodium hydroxymethylcellulose solution; after cooling to room temperature, the two or mixed according to a certain ratio, fully stirred until unif...

Embodiment 3

[0059] An oral microsphere loaded with exosomes derived from MSCs, the preparation method of which comprises the following steps:

[0060] (1) Preparation of ALG / CST outer core solution:

[0061] Weigh sodium alginate ALG and chitosan CST respectively, add each into double-distilled water to disperse, prepare sodium alginate solution and chitosan solution, and fully dissolve them respectively; after cooling to room temperature, mix the two according to a certain ratio, and stir thoroughly until uniform; the concentration of the ALG / CST outer core solution is 1% ALG / 1% CST.

[0062] (2) Preparation of ALG / CNC core solution:

[0063] Weigh sodium alginate ALG and sodium hydroxymethylcellulose CNC-Na respectively, add each into double-distilled water to disperse, and dissolve fully respectively to prepare sodium alginate solution and sodium hydroxymethylcellulose solution; after cooling to room temperature, the two or mixed according to a certain ratio, fully stirred until unif...

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Abstract

The invention relates to a preparation method and application of oral microspheres loaded with MSCs-derived exosomes. The preparation method comprises the following steps: (1) preparing an ALG / CST inner core solution; (2) preparing an ALG / CNC outer core solution; (3) separating exosomes derived from mesenchymal stem cells; and (4) preparing microspheres loaded with the MSCs exosomes. In the invention, hydrophobic ALG / CST and hydrophilic ALG / CNC are combined, the ALG / CST is used for wrapping the inter core of the exosomes, the defects of easy acidolysis of ALG, low drug loading of CNC andthe like are overcome, and the exosomes are effectively protected from being damaged by gastric acid and digestive juice. Meanwhile, with the pH-sensitive slow-release targeting property of small intestines, MSCs can be safely transported to the small intestines in an oral mode, damage repair, immunoregulation and anti-inflammatory effects are carried out by means of a small intestine absorption function, and the treatment of RA and other related autoimmune diseases is realized.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a preparation method and application of oral microspheres loaded with exosomes derived from MSCs. Background technique [0002] More and more studies have confirmed that exosomes derived from mesenchymal stem cells can promote angiogenesis, reduce inflammation, and repair damaged tissues by transmitting signaling molecules between cells. Compared with MSCs, exosomes have the advantages of better stability, high survival rate after transplantation therapy, and no obvious adverse reactions. In addition, exosomes contain a large number and variety of protein cytokines and bioactive molecules, which are the main way for MSCs to exert anti-inflammatory, anti-fibrosis and immune regulation functions. Therefore, MSCs exosomes have certain clinical application prospects in the prevention and treatment of immune system diseases such as systemic lupus erythematosus and its complication...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/50A61K47/36A61K47/38A61P19/02A61P37/02
CPCA61K9/0053A61K9/5036A61K9/5078A61K35/28A61P19/02A61P37/02
Inventor 孙凌云甘璟璟赵远锦
Owner NANJING DRUM TOWER HOSPITAL
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