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Magnetic fluorescent coding microsphere and preparation method thereof

A fluorescent coding, microsphere technology, applied in the direction of fluorescence/phosphorescence, material analysis by optical means, material excitation analysis, etc., can solve the problem of magnetic fluorescent coding microsphere, microsphere particle uniformity, good fluorescence performance, unfavorable industrial production, process Complex process and other problems, to achieve stable optical properties, good fluorescence performance, reduce quenching and shielding effects

Active Publication Date: 2020-07-17
深圳唯公生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the process is too complicated and the cost is high; or, there are few types of applicable dyes, which is not conducive to industrial production
Moreover, it is difficult for the magnetic fluorescent encoded microspheres obtained by the above preparation method to have the advantages of good uniformity of microsphere particles, good magnetic properties and good fluorescent properties.

Method used

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  • Magnetic fluorescent coding microsphere and preparation method thereof
  • Magnetic fluorescent coding microsphere and preparation method thereof
  • Magnetic fluorescent coding microsphere and preparation method thereof

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preparation example Construction

[0044] A method for preparing magnetic fluorescently encoded microspheres, comprising the steps of:

[0045] mixing long-chain fatty acids, medium-short-chain fatty acids and long-chain fatty acid iron, and heating to obtain the magnetic nanoparticles;

[0046] Dissolving monodisperse polymer microspheres in poor solvent α1 to obtain mixture A;

[0047] Dissolving the magnetic nanoparticles in a good solvent β1 to obtain a mixture B;

[0048] Mix the mixture A and the mixture B, and deswell with a poor organic solvent α2 after the reaction to obtain the magnetic polymer microspheres;

[0049] Dissolving the magnetic polymer microspheres in a poor solvent α3 to obtain a mixture C;

[0050] Dispersing the hydrophobic fluorescent dye in a good solvent β2 to obtain a mixture D;

[0051]The mixture C is mixed with the mixture D, and then deswelled with a poor organic solvent α4 to obtain the magnetic fluorescent encoded microspheres.

[0052] Wherein, the volume ratio of the po...

Embodiment 1

[0114] This embodiment provides a magnetic fluorescent coded microsphere and its preparation method

[0115] a. Preparation of long-chain fatty acid iron, such as iron oleate:

[0116] Add 5.4g FeCl to the system 3 , 18.3g sodium oleate, 40mL absolute ethanol, 70mL cyclohexane and 30mL deionized water, reflux at 85°C for 1h. After absorbing the deionized water at the bottom, wash it repeatedly 3 times with deionized water to obtain an iron oleate solution;

[0117] b. Preparation of magnetic nanoparticles with a particle size of 4nm:

[0118] Add 120 mL of octadecene to a solution of long-chain fatty acid iron, such as iron oleate, stir magnetically at 100°C to remove cyclohexane, then add 8 mL of long-chain fatty acid, such as oleic acid and 10 mL of n-octanoic acid, and heat up to 290°C for 8 hours. After stopping the reaction, wash with ethanol to remove octadecene and unreacted substances, and obtain a black precipitate, which is a magnetic nanoparticle with a particle ...

Embodiment 2

[0126] This embodiment provides a kind of magnetic polymer microsphere and preparation method thereof

[0127] a. Preparation of long-chain fatty acid iron, such as iron oleate:

[0128] Add 5.4g FeCl to the system 3 18.3g sodium long-chain fatty acid, such as sodium oleate, 120mL absolute ethanol, 120mL cyclohexane and 120mL deionized water, reflux at 70°C for 96h. After absorbing the deionized water at the bottom, wash with deionized water repeatedly 3 times to obtain long-chain fatty acid iron, such as iron oleate solution;

[0129] b. Preparation of magnetic nanoparticles with a particle size of 10nm:

[0130] Add 120 mL of octadecene to a long-chain fatty acid iron solution, such as iron oleate, and stir magnetically at 100°C to remove cyclohexane, then add 10 mL of long-chain fatty acid, such as oleic acid and 50 mL of n-octanoic acid, and heat up to 320°C for 6 hours. After stopping the reaction, wash with ethanol to remove octadecene and unreacted substances, and ob...

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Abstract

The invention relates to a magnetic fluorescent coding microsphere and a preparation method thereof. Firstly, during magnetic particle synthesis, medium-chain and short-chain fatty acids are used formodifying the surfaces of magnetic nanoparticles, so that the polarity of the surfaces of the magnetic nanoparticles is increased, the magnetic nanoparticles can be uniformly dispersed in a good solvent, and the particle size is more uniform; then, a two-step swelling method is adopted, which comprises the following steps: firstly, enabling magnetic nanoparticles to enter swelled polymer microspheres; enabling the fluorescent dye to enter the swollen polymer microspheres to form a 'magnetic particle core-fluorescent shell layer' structure, and reducing quenching and shielding effects of the magnetic particles on fluorescence to the greatest extent; then, by utilizing the swelling fading effect of the microspheres in a poor solvent, sealing cracks generated on the surfaces of the microspheres due to the swelling effect, avoiding leakage of magnetic nanoparticles and fluorescent dyes, greatly simplifying the sealing process, and obtaining the magnetic fluorescent coding microspheres withgood microsphere particle uniformity, good magnetic performance and good fluorescent performance.

Description

technical field [0001] The invention relates to the field of composite materials, in particular to a magnetic fluorescent coded microsphere and a preparation method thereof. Background technique [0002] Fluorescence-encoded microspheres can achieve grouping effects in detection instruments by loading different quantities of one or more fluorescent dyes with different optical properties in the microspheres. Magnetic fluorescently encoded microspheres refer to fluorescently encoded microspheres with magnetic properties, which can be magnetically sorted. In some finer detection systems, the detection limit can be greatly improved by removing unreacted substances through magnetic separation. At the same time, among many sorting methods, magnetic sorting has the characteristics of low cost, simple operation, and easy automation. [0003] At present, the methods for preparing magnetic fluorescent-encoded microspheres mainly include: [0004] (1) Copolymerization method: Mix th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432G01N2021/6439
Inventor 王建海李为公
Owner 深圳唯公生物科技有限公司
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