A kind of mucosal lactobacillus and its application in sewage desulfurization
A Lactobacillus mucosae, sewage technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria and other directions, can solve problems affecting the operating efficiency and stability of electrodes, and achieve improved operating efficiency and stability. Strong sulfur oxidizing ability and strong adaptability
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Embodiment 1
[0037] Embodiment 1, the oxygen demand fermentation test of bacteria
[0038] Simulate the environmental conditions of the electrochemical test (25 ℃, stand still), and under the condition of no external potential, first fill 6 500 ml anaerobic bottles with 400 ml of sodium sulfide at a concentration of 0.5 g / L (2 bottles), respectively. 0.75 g / L (1 bottle), 1.0 g / L (1 bottle), 1.25 g / L (1 bottle) and 1.5 g / L (1 bottle) of fermentation medium, and then inoculate each bottle with the strain Lactobacillus mucosae YCLS-3-5, so that the initial bacterial concentration is 4×10 7 cfu / ml to make a fermentation broth containing bacteria. One bottle of inoculum containing sodium sulfide concentration of 0.5 g / L and 1.5 g / L was taken, respectively, and filled with nitrogen for 3-5 min, as the anaerobic group, and the other 4 bottles were not filled with nitrogen, as the micro-aerobic group. In order to exclude the influence of oxygen on the transformation of sodium sulfide, all mic...
Embodiment 2
[0045] Embodiment 2, the test of the influence of different fermentation liquid loadings on the sulfur conversion of bacteria
[0046] To simulate the environmental conditions of the electrochemical test (25 ℃, stand still), under the condition of no external potential, first fill 200 ml, 300 ml, 400 ml and 500 ml of sodium sulfide in four 500 ml anaerobic bottles respectively. 1.0 g / L of fermentation medium, and then inoculate each bottle with the strain Lactobacillus mucosae YCLS-3-5, so that the initial bacterial concentration is 4×10 7 cfu / ml, made into fermented broth containing bacteria, as a test group for different fermented broth filling volumes. In order to compare the effect of oxygen on the conversion of sodium sulfide, all test groups were equipped with a blank control, except that sterile water was used instead of bacterial liquid, and the rest of the operations were the same as those of the test group. All the test groups and the blank control group were cult...
Embodiment 3
[0050] Example 3, Microbial Fuel Cell Determination of Electricity Production Ability Test of Bacterial Strains
[0051] Add 300 ml of deionized water to the two 400 ml anode chambers and cathode chambers respectively, put the carbon brush into the two chambers, and put the reference electrode in the cathode chamber. The solution in the reference electrode is saturated KCl, and the anode carbon brush and cathode A 10Ω resistor is connected between the carbon brushes, the anode chamber is the working electrode, and the cathode chamber is the auxiliary electrode, forming a microbial fuel cell.
[0052] After adding sterilized sulfur-free separation medium (that is, no sodium sulfide was added to the separation medium) into the anode chambers of the seven microbial fuel cells, 0 cfu / ml, 1×10 8 cfu / ml, 5×10 8 cfu / ml, 1×10 9 cfu / ml, 2.5×109 cfu / ml, 5×10 9 cfu / ml and 7.5×10 9 cfu / ml bacterial suspension. Use the data collector software to measure the electricity production capa...
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