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Method for remediating cadmium-polluted soil through combination of arbuscular mycorrhizal fungi and afforesting nursery stocks

A technology for arbuscular mycorrhizal fungi and cadmium-contaminated soil, applied in the agricultural field, can solve the problems of neglecting the fixation and remediation of soil heavy metals, failing to exert the remediation potential of AM fungi, and small biomass, so as to reduce the risk of secondary pollution and reduce the risk of secondary pollution. Improve repair efficiency and improve the effect of application

Inactive Publication Date: 2020-07-31
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Mainly utilize herbaceous plants in the above-mentioned technical scheme, such as Solanum nigrum and Sedum rosea, the biomass is relatively small
In addition, due to the method of harvesting the aboveground parts of plants, the fixing and repairing effects of plant roots and AM fungi on soil heavy metals have been ignored, especially the repair potential of AM fungi itself has not been brought into play.

Method used

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  • Method for remediating cadmium-polluted soil through combination of arbuscular mycorrhizal fungi and afforesting nursery stocks
  • Method for remediating cadmium-polluted soil through combination of arbuscular mycorrhizal fungi and afforesting nursery stocks
  • Method for remediating cadmium-polluted soil through combination of arbuscular mycorrhizal fungi and afforesting nursery stocks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The arbuscular mycorrhizal fungus inoculum used the G. mosesi fungus BGCAM001 provided by the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences.

[0027] The substrate is obtained by uniformly mixing 40wt% peat, 10wt% fine vermiculite, 45wt% pecan husk powder and 5wt% carbonized rice husk, wherein the particle size of the fine vermiculite is 20-40 mesh.

[0028] Cultivate cypress cutting seedlings to take root, sterilize the substrate at 121°C for 2 hours, then repeat the sterilization after 48 hours, and inoculate the arbuscular mycorrhizal fungal inoculant in the sterilized substrate at a mass ratio of 5%; Plant the rooted dragon cypress cutting seedlings in the substrate; after the dragon cypress grows steadily for 1 month, transplant it to 20 mg / kg cadmium contaminated soil for more than 12 months; remove the whole dragon cypress together with the substrate from cadmium pollution The soil is moved to green land and used as g...

Embodiment 2

[0030] The arbuscular mycorrhizal fungus inoculum used the G. mosesi fungus BGCAM001 provided by the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences.

[0031] The substrate is obtained by uniformly mixing 40wt% peat, 10wt% fine vermiculite, 45wt% pecan husk powder and 8wt% carbonized rice husk, wherein the particle size of the fine vermiculite is 20-40 mesh.

[0032] Cultivate cypress cutting seedlings to take root, sterilize the substrate at 121°C for 2 hours, and then repeat the sterilization after 48 hours. Inoculate the arbuscular mycorrhizal fungal inoculant in the sterilized substrate at a mass ratio of 7%; Plant the rooted dragon cypress cutting seedlings in the substrate; after 1 month of stable growth, the dragon cypress is transplanted to 50mg / kg cadmium contaminated soil for more than 12 months; the whole dragon cypress plant together with the substrate is free from cadmium pollution The soil is moved to green land and use...

Embodiment 3

[0034] The arbuscular mycorrhizal fungus inoculum used the G. mosesi fungus BGCAM001 provided by the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences.

[0035] The substrate is obtained by uniformly mixing 40wt% peat, 10wt% fine vermiculite, 45wt% pecan husk powder and 7wt% carbonized rice husk, wherein the particle size of the fine vermiculite is 20-40 mesh.

[0036] Cultivate cypress cutting seedlings to take root, sterilize the substrate at 121°C for 2 hours, then repeat the sterilization after 48 hours, and inoculate the arbuscular mycorrhizal fungal inoculant in the sterilized substrate at a mass ratio of 8%; Plant the rooted dragon cypress cutting seedlings in the substrate; after 1 month of stable growth, the dragon cypress is transplanted into 100mg / kg cadmium contaminated soil for more than 12 months; the whole cypress plant together with the substrate is free from cadmium pollution The soil is moved to green land and used as...

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Abstract

The invention belongs to the technical field of agriculture, and provides a method for remediating cadmium-polluted soil through combination of arbuscular mycorrhizal fungi and afforesting nursery stocks. Absorption of cupressaceae plants to cadmium is promoted by virtue of the arbuscular mycorrhizal fungi, meanwhile, absorption fixation of the AM fungi to the cadmium is taken as an auxiliary remediating measure, and the cadmium-polluted soil is remediated by transplanting the nursery stocks together with mycorrhizae in an afforesting process. According to the method, plant remediation is carried out in manners of the absorption fixation and transplantation afforesting of nursery stock roots and is perfectly combined with characteristics of a mycorrhiza technique, so that the remediation efficiency is improved, meanwhile, a root fixation effect of the nursery stocks can be achieved well, a secondary pollution risk is further reduced, and the practical applicability is improved.

Description

Technical field [0001] The invention belongs to the technical field of agriculture, and relates to a method for phytoremediation of heavy metal contaminated soil, in particular to a method for arbuscular mycorrhizal fungi combined with greening seedlings to repair cadmium contaminated soil. Background technique [0002] Arbuscular mycorrhizae (AM) fungi, namely AM fungi, are a kind of mutual symbiosis with plant root system and microbial characteristics in soil ecosystem. In recent years, plant-mycorrhizal fungi joint repair has become a hot spot in current phytoremediation research. [0003] For example: Chinese invention patents a method of using arbuscular mycorrhizal fungi to enhance the absorption of cadmium in the soil by nightshade [Patent No.: CN201410197384.0] provides an arbuscular mycorrhizal fungus that can improve the growth of nightshade and absorb heavy metals in the soil. The application of abilities and a method of using arbuscular mycorrhizal fungi to enhance the...

Claims

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Application Information

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IPC IPC(8): A01B79/02A01G17/00A01G18/10A01G24/28A01G24/15A01G24/25B09C1/10
CPCA01B79/02A01G17/005A01G18/10A01G24/28A01G24/15A01G24/25B09C1/105Y02A40/22Y02P60/40
Inventor 郭彬刘琛刘俊丽李凝玉林义成李华陈晓冬傅庆林
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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