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Preparation method and application of microbial cellulose membrane

A microbial cellulose and seed technology, applied in the field of microbial cellulose film preparation, can solve the problems of long time spent, limited industrial production cost, high cost of fermentation medium, etc., to promote growth, save processing time, and have wide application prospects Effect

Active Publication Date: 2020-07-31
无锡秋可生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the cost of microbial cellulose fermentation medium is relatively high, and the cost of industrial production is limited. The present invention uses rice protein slag, a by-product of starch sugar production, as raw material, with more than 60% protein and reasonable amino acid composition. It is a rare non-antigen plant protein. Source, while making full use of waste, it also improves the utilization rate of resources; and the method of manufacturing microbial cellulose film in the prior art is mainly to prepare and sterilize raw and auxiliary materials, inoculate and ferment culture under appropriate conditions to obtain microbial cellulose raw materials, the microorganism The cellulose raw material is bleached with a large amount of alkaline solution and disinfectant, rinsed with a large amount of acidic solution, neutralized and cleaned to obtain a microbial cellulose film. The production process requires a large amount of alkaline solution / acidic solution and water, and the operation process takes a long time. long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of preparation method of microbial cellulose film comprises the following steps:

[0030] Step 1, add coconut milk, rice protein residue and sucrose into pure water and be mixed with seed medium, the content of coconut milk in seed medium is 1.2wt%, the content of rice protein residue is 0.4wt%, the content of sucrose is 5wt%, the rest is pure water;

[0031] Step 2, using an inoculation loop to take 1 ring of Gluconacetobacter xylinum on the plate and insert it into the beaker seed medium in step 1, shake it well, and then culture it at 30°C for 48 hours to obtain the primary seed solution;

[0032] Step 3, add coconut milk, rice protein residue and sucrose into pure water and be mixed with seed culture medium, the content of coconut milk in seed culture medium is 1.2wt%, the content of rice protein residue is 0.4wt%, the content of sucrose is 5wt%, the rest is pure water; the seed medium is pasteurized at 60°C, the sterilization time is controlled at 30min, an...

Embodiment 2

[0050] A kind of preparation method of microbial cellulose film comprises the following steps:

[0051] Step 1, add coconut milk, rice protein residue and sucrose into pure water and be mixed with seed medium, the content of coconut milk in seed medium is 1.6wt%, the content of rice protein residue is 0.6wt%, the content of sucrose is 6wt%, the rest is pure water;

[0052] Step 2: Inoculate the Gluconacetobacter xylinum on the 1-ring plate with an inoculation loop into the beaker seed medium in Step 1, shake well, and then culture at 30°C for 36 hours to obtain primary seed liquid;

[0053] Step 3, add coconut milk, rice protein residue and sucrose into pure water and be mixed with seed medium, the content of coconut milk in seed medium is 1.6wt%, the content of rice protein residue is 0.6wt%, the content of sucrose is 6wt%, the rest is pure water; the seed medium is pasteurized at 65°C, the sterilization time is controlled at 25min, and cooled to 30°C after sterilization;

...

Embodiment 3

[0060] A kind of preparation method of microbial cellulose film comprises the following steps:

[0061] Step 1, adding coconut milk, rice protein residue and sucrose into pure water to prepare a seed medium, the content of coconut milk in the seed medium is 2wt%, the content of rice protein residue is 0.8wt%, and the content of sucrose is 8wt% %, the rest is pure water;

[0062] Step 2: Inoculate the Gluconacetobacter xylinum on the 1-ring plate with an inoculation loop into the beaker seed culture medium in Step 1, shake well, and then culture at 32°C for 24 hours to obtain the primary seed liquid;

[0063] Step 3, adding coconut milk, rice protein residue and sucrose into pure water to prepare a seed medium, the content of coconut milk in the seed medium is 2wt%, the content of rice protein residue is 0.8wt%, and the content of sucrose is 8wt% %, the rest is pure water; the seed medium is pasteurized at 70°C, the sterilization time is controlled at 20min, and cooled to 30°C...

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Abstract

The invention relates to a preparation method and application of a microbial cellulose membrane. The preparation method comprises the following steps: preparation of a seed culture medium, preparationof a primary seed solution, preparation of a fermentation seed solution, preparation of a primary microbial cellulose membrane and preparation of a fermentation-type microbial cellulose membrane. According to the method, the raw material utilization rate is 85-88%, and the yield of the microbial cellulose membrane is 65-75%. The microbial cellulose membrane can be applied to industries such as food industry, facial masks, biological medicines and sound vibration membranes, and has a very wide application prospect.

Description

technical field [0001] The invention relates to the technical field of development and application of microbial cellulose membranes, in particular to a preparation method and application of microbial cellulose membranes. Background technique [0002] The use of microbial fermentation and biosynthetic pathways to obtain cellulose was first discovered by scholar Brown in 1886. They found that when Acetobacter xylinum was cultured in a static way, a layer of white floating matter could be obtained. After taking out this kind of membranous substance, it can be confirmed that its structure and chemical properties are the same as cellulose . In order to distinguish it from common plant cellulose, this type of cellulose is named microbial cellulose. [0003] Compared with plant cellulose, microbial cellulose has many incomparable superior characteristics. At present, developed countries such as Japan and the United States are devoting themselves to the development and application...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/20A23L29/30H04R31/00A61K8/73A61K8/02A61Q19/00C12R1/02
CPCC12P19/04C12N1/20A23L29/30H04R31/003A61K8/731A61K8/0212A61Q19/00A23V2002/00A23V2250/5108
Inventor 于秋生何剑飞於慧利韩粉丽
Owner 无锡秋可生物科技有限公司
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