Supercharge Your Innovation With Domain-Expert AI Agents!

Method for rapidly detecting total ginkgolic acid in sample in ginkgo biloba extract production process

A total ginkgolic acid, production process technology, applied in the field of rapid detection of biomass, can solve the problems of affecting the service life of the chromatographic column, large injection volume, overloading of flavonoids and lactones, etc., to achieve short time consumption, reduced use, The effect of reducing pollution

Active Publication Date: 2020-08-04
劲牌持正堂药业有限公司
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] During the detection of ginkgolic acid, due to the large amount of flavonoids and lactones contained in the Ginkgo biloba extract (for example, the total flavonoids ≥ 24% in the "Chinese Pharmacopoeia", and lactone components ≥ 6%), in order to detect low-level total ginkgolic acids, the injection volume is extremely large, resulting in severe overloading of flavonoids and lactone components, affecting the service life of the chromatographic column, and producing a large amount of acetonitrile waste liquid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly detecting total ginkgolic acid in sample in ginkgo biloba extract production process
  • Method for rapidly detecting total ginkgolic acid in sample in ginkgo biloba extract production process
  • Method for rapidly detecting total ginkgolic acid in sample in ginkgo biloba extract production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1 Preparation method of reference substance solution:

[0065] Refer to the Summary of the Invention for the preparation method of the reference solution.

[0066] 2 Preparation method of the test solution:

[0067] For the preparation method of the test solution, see the Summary of the Invention.

[0068] Accurately draw 5 μl each of the reference solution, the test solution and the positioning control solution respectively, inject it into the liquid chromatograph, calculate the total peak area of ​​the chromatographic peak corresponding to the total ginkgolic acid reference substance in the test solution, and compare it with ginkgoic acid. Calculate the total ginkgolic acid content by the external standard method.

[0069] Use 1.0g, 1.5g and 2.0g of C18 filler to pack the column respectively, carry out sample pretreatment according to 2.1, collect 1mL of 60% ethanol cleaning solution tail liquid before elution with 95% ethanol, filter with 0.22μm filter membrane and...

Embodiment 2

[0071] Use 2.0g of C18 packing to load the column and load the sample until 20mL of 60% ethanol is finished cleaning the residual drug solution. Prepare three samples, and then use 10mL of methanol, 15mL of methanol, 20% of methanol and 25mL of 95% ethanol to wash at a speed of 2mL / min. After the elution and elution were completed, 1 mL of the eluted tail liquid was collected, and directly injected for detection after filtration with a 0.22 μm filter membrane. As a result, total ginkgolic acid was detected in the eluted tail liquid of the first three, and 25 mL of 95% ethanol eluted the tail liquid. Since the pollution of ethanol is less and the waste liquid is easier to handle, it is determined that the eluting solvent is 95% ethanol, and the dosage is 25mL.

Embodiment 3

[0073] Chromatographic column with octadecylsilane bonded silica gel (inner diameter 2.1mm, particle size 1.7 / 1.8μm), UV detector / DAD / PDA, λ=310nm, column temperature T=30℃, injection volume : 5 μL, using acetonitrile-0.1% trifluoroacetic acid solution as the mobile phase to carry out gradient elution according to Table 1, the flow rate of the mobile phase is 0.4mL / min, see the chromatogram of the reference solution for positioning figure 1 , the total ginkgolic acid contains ginkgoxinic acid and ingredients 2-5, a total of 5 ingredients.

[0074] Table 1 Gradient elution table

[0075] time / min Acetonitrile (%) 0.1% trifluoroacetic acid (%) 0 65 35 5.5 72 28 10 72 28 15 100 0 18 100 0 18.01 65 35 24 65 35

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for rapidly detecting a total ginkgolic acid in a sample in a ginkgo biloba extract production process. The method comprises the steps of rapid pretreatment and detection, can rapidly detect the total ginkgolic acid in a liquid sample and a solid sample in the ginkgo biloba extract production process, greatly shortens the index detection time, and realizes high continuity of production while effectively ensuring the product quality through real-time monitoring of a process sample.

Description

technical field [0001] The invention relates to the technical field of rapid detection of biomass, in particular to a rapid detection method for total ginkgolic acid in samples in the production process of ginkgo biloba extract. Background technique [0002] Ginkgo biloba extract is a medicinal extract recorded in the Chinese Pharmacopoeia, and its process is summarized as follows: (1) pretreatment of medicinal materials: take ginkgo leaves and crush them; (2) extraction: heat and reflux the crushed ginkgo leaves with dilute ethanol, Filtration, combining the filtrates into the extract liquid; (3) recovery of ethanol: the extract is concentrated under reduced pressure to recover ethanol, and continue to concentrate to an appropriate amount; (4) solid-liquid separation: the supernatant is obtained by extracting the concentrated liquid through centrifugation / filtration; (5) Resin adsorption and elution: the supernatant is added to the macroporous adsorption resin column that h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8679
Inventor 严书超胡佑彪王玉陈志元黄茜茜
Owner 劲牌持正堂药业有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com