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A compound for preventing and treating Tetranychus cinnabarinus extracted from branches and leaves of peanuts and its application

A technology of Tetranychus cinnabarinus and peanut branches and leaves, applied in the field of botanical pesticides and agricultural acaricides, to achieve the effects of reducing economic losses, reducing the degree of damage, and avoiding damage

Active Publication Date: 2022-01-25
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to develop a method for separating and extracting an active compound from the crude peanut extract and preparing an acaricide by adding an auxiliary agent and applying it to the control of Tetranychus cinnabarinus, Effectively control Tetranychus cinnabarinus, reduce the degree of harm, reduce economic losses in agricultural production, and have the characteristics of no pollution, no pollution and no residue, and avoid the harm of chemically synthesized pesticides

Method used

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  • A compound for preventing and treating Tetranychus cinnabarinus extracted from branches and leaves of peanuts and its application
  • A compound for preventing and treating Tetranychus cinnabarinus extracted from branches and leaves of peanuts and its application
  • A compound for preventing and treating Tetranychus cinnabarinus extracted from branches and leaves of peanuts and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The active compound extracted from the crude extract of peanut branches and leaves was applied to the control test of Tetranychus cinnabarinus:

[0039] Weigh 20 g of the crude extract of peanut branches and leaves, use silica gel as the stationary phase, and use petroleum ether-ethyl acetate (volume ratio of 1:0-0:1) and ethyl acetate-methanol (volume ratio of 1:0- 0:1), to obtain a variety of crude extracts; using medium pressure column chromatography, using C18 as the stationary phase, mobile phase methanol-water (volume ratio of 1:0-0:1) or chloroform-methanol (volume ratio 1:0-0:1) to separate and purify the crude extract to obtain a purified product; using high performance liquid chromatography, the active compound is determined to be palmitic acid by comparing with a standard. The compound obtained from the above separation and purification was used to carry out the control test on Tetranychus cinnabarinus. The test method was carried out by dipping the stem of t...

Embodiment 2

[0041] The active compound extracted from the crude extract of peanut branches and leaves was applied to the control test of Tetranychus cinnabarinus:

[0042] Weigh 20 g of the crude extract of peanut branches and leaves, use silica gel as the stationary phase, and use petroleum ether-ethyl acetate (volume ratio of 1:0-0:1) and ethyl acetate-methanol (volume ratio of 1:0- 0:1), to obtain a variety of crude extracts; using medium pressure column chromatography, using C18 as the stationary phase, mobile phase methanol-water (volume ratio of 1:0-0:1) or chloroform-methanol (volume ratio 1:0-0:1) to separate and purify the crude extract to obtain a purified product; using high performance liquid chromatography, the active compound is determined to be isovanillic acid by comparing with a standard. The compound obtained from the above separation and purification was used to carry out the control test on Tetranychus cinnabarinus. The test method was carried out by dipping the stem o...

Embodiment 3

[0044] The active compound extracted from the crude extract of peanut branches and leaves was applied to the control test of Tetranychus cinnabarinus:

[0045] Weigh 20 g of the crude extract of peanut branches and leaves, use silica gel as the stationary phase, and use petroleum ether-ethyl acetate (volume ratio of 1:0-0:1) and ethyl acetate-methanol (volume ratio of 1:0- 0:1), to obtain a variety of crude extracts; using medium pressure column chromatography, using C18 as the stationary phase, mobile phase methanol-water (volume ratio of 1:0-0:1) or chloroform-methanol (volume ratio 1:0-0:1) to separate and purify the crude extract to obtain a purified product; the active compound is determined to be β-sitosterol by comparison with a standard by using high performance liquid chromatography. The compound obtained from the above separation and purification was used to carry out the control test on Tetranychus cinnabarinus. The test method was carried out by dipping the stem of...

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Abstract

The first aspect of the present invention discloses a compound for preventing and controlling Tetranychus cinnabarinus extracted from branches and leaves of peanuts. The compound for preventing and controlling Tetranychus cinnabarinus includes palmitic acid, isovanillic acid, β-sitosterol and stigmasterane-3,6-diol. The second aspect of the present invention discloses the extraction method of the compound. The third aspect of the present invention discloses the method of using the compound. The fourth aspect of the present invention discloses a preparation method of the compound acaricide. The compound for preventing and controlling Tetranychus cinnabarinus extracted from branches and leaves of peanuts provided by the invention and its application can effectively prevent and control Tetranychus cinnabarinus, reduce the degree of environmental damage, and reduce economic losses in agricultural production.

Description

technical field [0001] The invention relates to the technical field of botanical pesticides and agricultural acaricides, in particular to a compound for preventing and controlling Tetranychus cinnabarinus extracted from branches and leaves of peanuts and its application. Background technique [0002] Cinnabar spider mite (Tetranychus cinnabarinus): also known as spider mite, belongs to Acaridae, Tetranychus family; its growth and development characteristics are small size, fast reproduction, multiple generations (one generation in 10 days in summer), strong adaptability, and easy to produce drug resistance; cinnabar There are as many as 146 species of spider mites host plants in 45 families (cotton, soybean, vegetables, fruit trees, flowers, etc.). Under high temperature and low humidity conditions, they damage leaves and make them turn green and yellow. In severe cases, leaves dry up or even fall off, reducing yield and quality. [0003] At present, chemical pesticides cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/20A01N37/02A01N37/40A01N45/00C07C51/42C07J9/00A01P7/02A01G7/06A01G13/00
CPCA01N65/00A01N65/20A01N37/02A01N37/40A01N45/00C07C51/42C07J9/00A01G7/06A01G13/00C07C53/126C07C65/21
Inventor 代光辉高彦瑾刘雅雅刘佳
Owner SHANGHAI JIAOTONG UNIV
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