Thyroid cancer auxiliary molecule diagnosis test kit and use method

Abstract

The invention provides a thyroid cancer auxiliary molecule diagnosis test kit. The thyroid cancer auxiliary molecule diagnosis test kit comprises amplification primers and probes for detecting BRAF, HRAS, KRAS, NRAS and TERT genes, internal control gene ACTB primers and probes, and external control gene BRAF primers and probes; the primers are specifically used for detecting a V600E site of the BRAF gene, a Q61R site of the HRAS gene, a G12C site of the KRAS gene, a G12D site of the KRAS gene, a G13R site of the KRAS gene, a G13D site of the KRAS gene, a Q61K site of the NRAS gene, a Q61P siteof the NRAS gene, a Q61H1 site of the NRAS gene, a Q61H2 site of the NRAS gene, a C228T site of the TERT gene and a C250T site of the TERT gene. The invention also provides a use method of the thyroid cancer auxiliary molecule diagnosis test kit. The problems of sensitivity, cost, operation convenience and the like of an existing thyroid cancer auxiliary molecule diagnosis test kit are solved.

Description

technical field [0001] The invention is applied in the technical field of gene detection, and specifically relates to a thyroid cancer auxiliary molecular diagnosis kit and a use method. Background technique [0002] Thyroid nodule is a common thyroid disease, which is caused by local abnormal growth of thyroid cells. In most cases, thyroid nodules are benign and can be treated conservatively, but 5%-10% of thyroid nodules are malignant and require early surgical treatment in order to obtain a good prognosis. Fine-needle aspiration cytology (FNA) is the most reliable method for evaluating benign and malignant thyroid nodules. However, there are still 20%-30% of thyroid nodules that cannot be diagnosed as benign or malignant by FNA. Clinically, there is a lack of reliable diagnostic methods for such nodules, and most patients undergo diagnostic surgery to determine the pathology of the nodules. If the tumor is larger than 1 cm, a second operation is required to complete th...

AI Technical Summary

Problems solved by technology

The principle is that the primer extension guided by DNA polymerase starts from the 3' end of the primer in the PCR process, and the degree of complementary pairing between the base at the 3' end of the primer and the template strongly affects the recognition of the polymerase and the progress of the PCR reaction: if This base is normally complementary to the template (A-T, G-C), the primer can be extended without interruption, PCR can be efficiently performed, and a complete product is obtained; on the contrary, if this base is not normally paired with the template, the extension of the primer is blocked , the PCR efficiency is greatly reduced

Conventional ARMS-PCR technology is easy to operate, the detection time is short, and the detection sensitivity is generally around 1%. If combined with fluorescence quantitative technology, the detection efficiency can reach 0.5%.

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