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Method for quickly identifying Daqu grades

A large-scale and graded technology, applied in character and pattern recognition, preparation of test samples, instruments, etc., can solve the problems of low detection sensitivity, poor repeatability, and low accuracy

Pending Publication Date: 2020-08-14
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent (CN103293264A, a method for identifying the quality of Daqu) introduces a combination of solid-phase microextraction and gas chromatography-mass spectrometry, which can distinguish yellow koji, white koji, and finished koji, but the accuracy is not high
The high-throughput DNA sequencing technology developed rapidly in recent years can obtain the composition of microbial communities. Applying this technology to the research of Daqu microorganisms can reveal the relationship between the structure of microbial communities and the quality of Daqu (Chinese patent CN104109719A, a method based on boxplot A method for discriminating the quality of Daqu; CN104372075A, a method for constructing a discrimination model to discriminate the quality of Daqu; CN109949863A, a method for discriminating the quality of Daqu based on a random forest model), the current detection sensitivity of this technology is slightly low, and the repeatability is poor

Method used

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  • Method for quickly identifying Daqu grades
  • Method for quickly identifying Daqu grades
  • Method for quickly identifying Daqu grades

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The preparation process of the Daqu sample is as follows: adding distilled water to the Daqu sample, grinding, centrifuging, taking the supernatant and adding it to the NMR tube, and then adding the heavy water containing 0.05% deuterated trimethylsilyl sodium propionate (TMSP) internal standard, Mix well, seal, refrigerate, to be tested, 1 The measurement conditions of H-NMR were as follows: the measurement frequency was 600.13 MHz, the temperature was 298 K, the scanning range was -5 to 15 ppm, the sampling point was 64 k, and the number of scanning was 64. The grinding process is as follows: grinding at 70 Hz for 300 s, with two cycles; the centrifugation process is: centrifugation at 10000 rpm for 10 min.

[0026] When using partial least squares discriminant analysis: use partial least squares discriminant analysis O2PLS-DA to analyze the data in the standard sample NMR integral database to establish a classification model; Unknown Daqu samples were identified.

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Embodiment

[0031] 1. Measure the sample to be tested

[0032] 1.1 Daqu Materials:

[0033] In this example, a total of 80 samples of Luzhou Huaiyu Koji Co., Ltd. were collected from Luzhou Huaiyu Koji Co., Ltd. with different storage periods. Among them, 65 samples were used as training sets and 15 samples were used as test sets. , esterification force, ammonia nitrogen, bulk density) were graded, and the details are shown in Table 1 below. The selected Daqu pieces were pulverized, passed through a 0.25mm aperture sieve, and stored in a -80°C cryogenic refrigerator for later use. Heavy water used in the test (D 2 O) and sodium deuterated trimethylsilyl propionate (TMSP-d4) were purchased from Cambridge Isotope Laboratory, and the NMR spectrum was measured by Bruker AVANCEIII 600MHz superconducting nuclear magnetic resonance spectrometer.

[0034] Table 1 Classification and statistics of Luxiang Daqu

[0035]

[0036] 1.2 Sample preparation:

[0037] The optimal method is confirme...

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PUM

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Abstract

The invention relates to the technical field of Daqu identification, and in particular relates to a method for rapidly identifying Daqu grades. The specific technical scheme is as follows: the methodfor quickly identifying the Daqu grades comprises the steps of collecting nuclear magnetic spectra of a plurality of Daqu samples with different grades, correcting the nuclear magnetic spectra, and carrying out piecewise integration; then constructing a Daqu grade identification classification model; and finally, inputting the nuclear magnetism data of the unknown Daqu into the classification model to realize rapid identification of Daqu grades. According to the invention, a 1H-NMR technology is applied, a 1H-NMR integral database is established, a Daqu grade identification and classificationmodel is constructed, and finally, the Daqu grade identification and classification model is combined with a computer to identify Daqu grades.

Description

technical field [0001] The invention relates to the technical field of Daqu identification, in particular to a method for quickly identifying Daqu grades. Background technique [0002] Daqu is a micro-ecological product with a large shape and a variety of fungi, enzymes and aroma substances, which is mainly made of wheat or supplemented by fermentation of peas and barley. It is a saccharification starter and aroma agent in wine production. It is also part of the raw materials for wine production. Due to the metabolism of microorganisms, Daqu is rich in flavor substances and their precursors. These flavor compounds constitute Daqu's own "Quxiang", which has a significant impact on the production of Chinese liquor. [0003] The quality evaluation of Daqu is mainly based on the traditional sensory evaluation method supplemented by physical and chemical analysis. Although it can reflect the quality of Daqu to a certain extent, it lacks some scientific basis. The physical and ch...

Claims

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Application Information

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IPC IPC(8): G01N24/08G01N1/28G06K9/62G06N3/04
CPCG01N24/08G01N1/286G01N2001/2866G06N3/045G06F18/214G06F18/2411Y02A90/30
Inventor 王寿峰明红梅周慧周冯利
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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