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Detection test paper for detecting brucella antibody

A Brucella and detection test paper technology, applied in the field of epidemic detection, can solve the problems of long detection time, low accuracy rate, and difficult judgment, and achieve the effect of fast detection speed, low cost, and strong anti-interference

Pending Publication Date: 2020-08-14
武汉优恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tiger red plate agglutination test is highly subjective, difficult to judge, and the accuracy rate is low
The test tube agglutination test is more accurate, but the operation is complicated and the detection time is longer, > 24 hours

Method used

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  • Detection test paper for detecting brucella antibody
  • Detection test paper for detecting brucella antibody

Examples

Experimental program
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preparation example Construction

[0032] Preparation of the sample pad 2 of the present invention: soak the glass fibers in the sample pad treatment solution (phosphate buffer containing 5% sucrose, 0.5% Casein, 0.09% Proclin300), soak both sides for no less than 5 minutes, and soak evenly Place the glass fiber flat on the rack, dry at 37°C, put it into an aluminum foil bag with a desiccant, seal it, and set it aside.

[0033] Colloidal gold pad 3 of the present invention adopts following method to make:

[0034] 1) Preparation of gold particle-labeled Brucella antigen 1: take 10ml of nano-gold particles, add Brucella antigen 1 to make the final concentration 5μg / ml-50μg / ml, mix and stir at room temperature for 30min; then add 2ml of labeling and blocking solution (PBS buffer containing 2% BSA), mixed and stirred at room temperature for 30min, then centrifuged and purified to obtain gold particle-labeled Brucella antigen 1;

[0035] 2) Preparation of chicken IgY labeled with gold particles: take 10ml of gold ...

Embodiment 1

[0046] Preparation of colloidal gold pad 3:

[0047] 1) Preparation of gold particle-labeled Brucella antigen: take 10ml of 30nm nano-gold particles and 10ml concentration of 50μg / ml Brucella antigen, mix, react at room temperature for 15min, and then centrifuge and purify to obtain gold particle-labeled Brucella antigen. bacterial antigens;

[0048] 2) Preparation of chicken IgY labeled with gold particles: take 10ml of 30nm gold nanoparticles and 10ml of chicken IgY with a concentration of 100 μg / ml, mix them, react at room temperature for 15min, and then centrifuge and purify to obtain chicken IgY labeled with gold particles;

[0049] 3) Preparation of colloidal gold pad: Mix equal volumes of gold particle-labeled Brucella antigen and gold particle-labeled chicken IgY, then add them into the colloid and set aside.

[0050] Treatment of nitrocellulose membrane 4:

[0051] The test line 5 and the quality control line 6 were scratched on the nitrocellulose membrane 4, the Br...

Embodiment 2

[0055] Preparation of colloidal gold pad 3:

[0056] 1) Preparation of gold particle-labeled Brucella antigen: take 10ml of 50nm nano-gold particles and 10ml concentration of 30μg / ml Brucella antigen, mix, react at room temperature for 15min, and then centrifuge and purify to obtain gold particle-labeled Brucella antigen. bacterial antigens;

[0057] 2) Preparation of chicken IgY labeled with gold particles: take 10ml of 50nm gold nanoparticles and 10ml of chicken IgY with a concentration of 90 μg / ml, mix them, react at room temperature for 15min, and then centrifuge and purify to obtain chicken IgY labeled with gold particles;

[0058] 3) Preparation of colloidal gold pad: Mix equal volumes of gold particle-labeled Brucella antigen and gold particle-labeled chicken IgY, then add them into the colloid and set aside.

[0059] Treatment of nitrocellulose membrane 4:

[0060] The test line 5 and the quality control line 6 were scratched on the nitrocellulose membrane 4, the Bru...

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Abstract

The invention relates to detection test paper for detecting a brucella antibody. The detection test paper comprises a bottom plate; a sample pad, a colloidal gold pad, a nitrocellulose membrane and awater absorption pad are sequentially fixed on the bottom plate; the colloidal gold pad is coated with a gold-particle-labeled brucella antigen 1 and a gold-particle-labeled chicken IgY; the nitrocellulose membrane is sprayed with a detection line and a quality control line, the detection line is coated with a brucella antigen 2, and the quality control line is coated with a goat anti-chicken IgYantibody. The detection test paper is high in brucella detection specificity, samples are not limited by species, samples of multiple species such as human, cattle and sheep can be detected, and the detection test paper is convenient to operate and high in detection speed.

Description

technical field [0001] The invention relates to the technical field of disease detection, in particular to a test paper for detecting Brucella antibodies. Background technique [0002] Brucellosis (Brucellosis, referred to as brucellosis) is a zoonotic disease characterized by abortion and fever caused by Brucella or Brucella, which seriously threatens humans and various animals. life and health. This disease not only has serious harm to the reproduction and production performance of animals, but more importantly, it is often difficult to cure people infected with Brucella, thus causing serious public health problems. Therefore, in countries where Brucella is endemic, elimination of brucellosis has been one of the most important goals of public health programs. Brucellosis is a worldwide zoonotic infectious disease with a wide range of prevalence, almost all over the world, and brucellosis is prevalent in all areas with livestock. In my country, cattle and sheep are the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558G01N33/532G01N33/531
CPCG01N33/6854G01N33/587G01N33/56911G01N33/558G01N33/532G01N33/531G01N2333/23G01N2469/20
Inventor 冷毅斌胡勤芹夏红星冯亮
Owner 武汉优恩生物科技有限公司
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