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Wood frog oil polysaccharide component and application thereof

A technology of Rana Rana oil and polysaccharide, which is applied to medical preparations, drug combinations, organic active ingredients and other directions containing active ingredients, can solve problems such as insufficient research on Rana Rana oil polysaccharides, etc., and can increase the generation of nitric oxide (NO) The effect of enhancing the phagocytic ability of macrophages, good safety profile

Active Publication Date: 2020-08-18
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In summary, the current research on Rana oil polysaccharides is not deep enough, and the tracking, separation, structural characteristics and activity evaluation of functional components need to be further explored

Method used

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  • Wood frog oil polysaccharide component and application thereof
  • Wood frog oil polysaccharide component and application thereof
  • Wood frog oil polysaccharide component and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Extraction and Purification of Rana Oil Crude Polysaccharide

[0033]With reference to the method mentioned in Yang Jing's master's degree thesis (extraction conditions optimization of crude polysaccharides from Rana Rana oil and research on its pharmacological activity, Jilin Agricultural University, 2011), weigh 10g of Rana Rana Oil defatted powder, and use a liquid-to-material ratio of 100:1 Soak in distilled water for 12 hours, homogenize, adjust pH to 6.8, add Rana oil degreasing powder 0.2% w / w papain and incubate at 37°C for 2 hours; inactivate enzyme in boiling water bath for 10 minutes, cool and centrifuge to take supernatant, adjust pH to 7.0, concentrated volume to 1 / 4, add 3 times the volume of 95V% ethanol aqueous solution, stand at 4°C for 12 hours; centrifuge to collect the precipitate, and freeze-dry to obtain the crude polysaccharide of Rana oil, with a yield of 5.09±0.21%, The total sugar content is 23.08±1.56%. The crude polysaccharide was ...

Embodiment 2

[0034] Example 2 Separation of Rana Oleopolysaccharide Components

[0035] Weigh 100 mg of the purified polysaccharide from Rana oil, dissolve it in 20 mL of deionized water, and load it onto a DEAE cellulose-52 chromatographic column (2.6 cm × 60 cm); Gradient elution was performed at a flow rate of 1 mL / min, 5 mL per tube; the absorbance at a wavelength of 490 nm was detected by the phenol-sulfuric acid method, and the elution curve was drawn. Such as figure 1 As shown, when different concentrations of NaCl solutions are used for gradient elution, three elution peaks are obtained respectively, that is, the elution peaks POR-1 to 3 corresponding to the elution of 0.1mol / L to 0.3mol / L NaCl solution; Collect and combine the eluents corresponding to POR-1~3 respectively to obtain POR-1 (0.1mol / L NaCl solution), POR-2 (0.2mol / L NaCl solution), POR-3 (0.3mol / L NaCl solution) of the three polysaccharide fractions; dialyze the three polysaccharide fractions three times with deioni...

Embodiment 3

[0036] Example 3 Composition Analysis and Antioxidant Activity Comparison of Rana Oil Polysaccharide Components

[0037] The total sugar content of each polysaccharide component is measured using the phenol-sulfuric acid method (Anal.Chem.28 (3) (1956) 350-356); the protein content is measured using the Coomassie Brilliant Blue method (Anal.Biochem.72 (1976) 248- 254); the content of uronic acid was determined by m-hydroxybiphenyl method (Anal.Biochem.54(2)(1973)484-489).

[0038] Determination of hydroxyl radical scavenging rate: Take 2mL polysaccharide component solutions with different concentrations (0.2mg / mL~1.0mg / mL), 1mL phosphate buffer (pH 7.4) containing 0.75mmol / L phenanthroline and 1mL 0.75mmol / L FeSO 4 solution, mix well, then add 1mL of 0.12% H 2 o 2 The solution was used to initiate the reaction, incubated at 37°C for 60 min, and the absorbance value at 536 nm was measured. The sample control tube is distilled water instead of the sample, and the sample back...

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Abstract

The invention provides a wood frog oil polysaccharide component. The wood frog oil polysaccharide component is a sugar-protein compound, the total sugar content is 80.87 + / - 1.17%, the uronic acid content is 10.19 + / - 0.43%, and the protein content is 6.42 + / - 0.16%; the wood frog oil polysaccharide component is hetero-polysaccharide and consists of mannose, glucosamine, galacturonic acid, galactose and fucose; the wood frog oil polysaccharide component is poly-dispersion polysaccharide, the weight-average molecular weight of the wood frog oil polysaccharide component is 999.586 kDa, the number-average molecular weight of the wood frog oil polysaccharide component is 244.366 kDa, and the poly-dispersion index of the wood frog oil polysaccharide component is 4.09. The wood frog oil polysaccharide component is pyranose, and the connection mode comprises alpha- and / or beta- configuration glycoside bonds. The wood frog oil polysaccharide component has remarkable effects of resisting oxidation and enhancing body immunity, and can be used for preparing functional products such as tablets, capsules, oral liquid and the like.

Description

technical field [0001] The invention belongs to the field of natural product preparation and application, and in particular relates to a wood frog oil polysaccharide component and its application. Background technique [0002] The impact of reactive oxygen species (ROS) on the immune system has two sides: at low concentrations, it regulates the immune response and cell proliferation to control the attack of self-reactive T cells on self-tissues, organs and cells; at high concentrations, it It directly invades activated T cells, destroys the membrane structure of immune cells, and leads to immune dysfunction. Although endogenous antioxidant enzymes and their related substances can reduce the damage of excess ROS to the body to a certain extent, in the face of oxidative stress, these endogenous antioxidant enzymes and their substances often cannot reach the minimum effective level. concentration. Therefore, in the state of oxidative stress, exogenous supplementation of antio...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07K2/00C12P21/06A61K38/02A61P39/06A61P37/04A61K31/715
CPCA61K31/715A61K38/02A61P37/04A61P39/06C07K2/00C08B37/0003C08B37/006C12P21/005C12P21/06A61K2300/00
Inventor 张扬虞晓瑾刘蕾张姝姝王佳怡唐月瑶任冰倩
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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