Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing dipeptidyl peptidase IV inhibitory active peptide from walnut meal and application thereof

A dipeptidyl peptidase and inhibitory activity technology, applied in the field of plant protein deep processing, can solve problems such as waste of walnut resources, and achieve the effects of good functional properties, high product yield and protein content, and strong inhibitory activity

Active Publication Date: 2020-08-18
JIANGNAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In recent years, the market demand for walnut oil has been increasing. As a by-product of walnut oil processing, defatted walnut meal has a protein content of more than 50%. waste

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing dipeptidyl peptidase IV inhibitory active peptide from walnut meal and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Alcohol washing: Add 6 times the volume of degreased walnut meal powder to an ethanol solution with a volume concentration of 85%, stir and wash at 50°C for 1 hour, and then filter with suction; take out the filter cake and dry it at 40°C to remove residual Ethanol, that is, alcohol-washed walnut meal.

[0040] (2) Pickling: Mix the alcohol-washed walnut meal with deionized water to prepare a suspension with a mass concentration of 10%, adjust the pH to 4.5, stir for 1 h, and centrifuge at 3000 r / min for 20 min to collect the precipitate; pickling 4 times, the precipitate was collected to obtain walnut protein curd.

[0041] (3) Shearing: Mix the walnut protein curd with deionized water to prepare a suspension with a mass concentration of 10%, adjust the pH to 9.0, set the temperature at 55°C, set the shear time to 5 min, and the shear speed to 10,000 r / min.

[0042] (4) Hydrolysis: Then add alkaline protease (E / S=2.0%), keep the temperature (55°C) and pH (9.0) c...

Embodiment 2

[0046] (1) Alcohol washing: Add 5 times the volume of degreased walnut meal powder to an ethanol solution with a volume concentration of 85%, stir and wash at 45°C for 1.5 h, and then filter with suction; take out the filter cake and dry it at 40°C to remove residual Ethanol, that is, alcohol-washed walnut meal.

[0047] (2) Pickling: Mix the alcohol-washed walnut meal with deionized water to prepare a suspension with a mass concentration of 10%, adjust the pH to 5.0, stir for 1 h, and centrifuge at 3000 r / min for 20 min to collect the precipitate; pickling 3 times, the precipitate was collected to obtain walnut protein curd.

[0048] (3) Shearing: Mix walnut protein curd with deionized water to prepare a suspension with a mass concentration of 9%, adjust the pH to 8.5, the temperature is 55°C, the shearing time is 6 min, and the shearing speed is 9000 r / min.

[0049] (4) Hydrolysis: To obtain walnut protein suspension, add alkaline protease (E / S=2.0%), keep the temperature...

Embodiment 3

[0053] (1) Alcohol washing: Add 5 times the volume of degreased walnut meal powder to an ethanol solution with a volume concentration of 85%, stir and wash at 40°C for 2.0 h, and then filter with suction; take out the filter cake and dry it at 35°C to remove residual Ethanol, that is, alcohol-washed walnut meal.

[0054] (2) Pickling: Mix the alcohol-washed walnut meal with deionized water to prepare a suspension with a mass concentration of 10%, adjust the pH to 5.5, stir for 1 h, and centrifuge at 4000 r / min for 20 min to collect the precipitate; pickling 2 times, the precipitate was collected to obtain walnut protein curd.

[0055] (3) Shearing: Mix walnut protein curd with deionized water to prepare a suspension with a mass concentration of 10%, adjust the pH to 9.0, set the temperature at 60°C, set the shear time to 5 min, and the shear speed to 12000 r / min, to obtain walnut protein suspension.

[0056] (4) Hydrolysis: Add alkaline protease (E / S=1.5%), keep the tempera...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing dipeptidyl peptidase IV inhibitory active peptide from walnut meal and an application thereof, which belongs to the technical field of plant protein deepprocessing. The method comprises the following steps: by taking degreased walnut meal as a raw material, firstly, carrying out alcohol washing, acid pickling and high-speed shearing treatment, and adding alkaline protease for hydrolysis to obtain enzymatic hydrolysate; and desalting and drying to obtain the dipeptidyl peptidase IV inhibitory active peptide. The walnut peptide prepared by the method provided by the invention has good hypoglycemic and antioxidant functional activity, high product yield and high protein content, realizes deep processing and comprehensive utilization of walnut meal, and has important significance for increasing the additional value of the walnut meal.

Description

technical field [0001] The invention relates to a method for preparing dipeptidyl peptidase IV inhibitory active peptide by using walnut dregs and an application thereof, belonging to the technical field of plant protein deep processing. Background technique [0002] Diabetes mellitus (DM), a metabolic disease associated with hyperglycemia, is a common disease worldwide. There are two types of diabetes, insulin-dependent diabetes (type I diabetes) and non-insulin-dependent diabetes (type II diabetes). Type 1 diabetes mellitus (T1DM) is caused by the inability of the pancreas to secrete insulin due to an autoimmune response that destroys the beta cells of the pancreas. Type 2 diabetes mellitus (T2DM) is a complex disease caused by insufficient insulin production or the inability of the body to utilize the insulin produced. The incidence of type II diabetes is gradually increasing, accounting for about 90% to 95% of diagnosed cases, causing a heavy burden on society, health ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC12P21/06C07K1/14C07K1/34Y02P60/87
Inventor 孔祥珍张丽娜华欲飞宋伟光张彩猛陈业明李兴飞
Owner JIANGNAN UNIV