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Co-assembled artificial periosteum and preparation method thereof

A co-assembly and artificial bone technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of poor mechanical properties, collapse and degradation rate, too fast, etc., and achieve the effect of improving mechanical properties

Active Publication Date: 2020-08-25
ASIA BIOMATERIALS WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, clinically, the method of inducing periosteum is used for bone repair, and the effect is good, but there are few artificial periosteums that can be used clinically, mainly mineralized collagen membranes, which have poor mechanical properties, and their mechanical properties will deteriorate after being wet. The problem of significant changes, resulting in adverse effects such as the risk of collapse during surgery and excessive degradation rates

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  • Co-assembled artificial periosteum and preparation method thereof
  • Co-assembled artificial periosteum and preparation method thereof
  • Co-assembled artificial periosteum and preparation method thereof

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preparation example Construction

[0071] The embodiment of the present invention also provides a preparation method of the co-assembled artificial periosteum, including:

[0072] Step 1. Obtain a co-assembly solution, which may specifically include two schemes:

[0073] Option One:

[0074] Obtain silk fibroin solution, phosphorus source solution, calcium source solution and silicon source;

[0075] Mixing the silk fibroin solution with a phosphorus source solution to obtain a silk fibroin-phosphorous solution;

[0076] mixing the calcium source solution with the silicon source to obtain a calcium-silicon solution;

[0077] Add the silk fibroin-phosphorous solution dropwise into the calcium-silicon solution, adjust the pH value to 7.4, continue the reaction for a period of time, stop stirring, let it stand, collect the precipitate, and then wash and centrifuge to obtain silk fibroin Protein-mediated silicon-doped hydroxyapatite sol (SF-SiHAp sol for short);

[0078] Stir and mix the silk fibroin-mediated s...

Embodiment 1

[0096] Example 1 Col-SiHAp-SF co-assembles artificial periosteum

[0097] Step 1. Weigh 1g of atelopeptide type I collagen, dissolve it completely in 0.2L of 0.5mol / L acetic acid solution, and take a water bath at 37°C; add 0.11g of silicon orthoacetate solid, stir and hydrolyze for 4 hours; weigh 3.52g of nitric acid tetrahydrate Dissolve calcium in the Col-silicon hydrolysis solution, mix for 3 hours at a stirring speed of 100rpm, adjust the pH of the solution to about 7.4, and prepare a collagen-calcium-silicon solution (Col-Ca-Si); weigh 1.12g of diammonium hydrogen phosphate and dissolve in Add diammonium hydrogen phosphate solution slowly to the Col-Ca-Si solution in 0.2L of purified water to keep the pH of the system at about 7.4. 3ml / min, the stirring speed is 100rpm; continue to stir for 3h after the phosphate salt is added dropwise, keep the system pH=7.4, and let it stand for 4h at 37°C; pour off the supernatant after aging, and wash the precipitate until neutral. ...

Embodiment 2

[0104] Example 2 SF-SiHAp-Col co-assembles artificial periosteum

[0105] Step 1. Weigh 1g silk fibroin, dissolve it completely in 18mL9.3mol / L lithium bromide solution, dialyze and centrifuge; take a small amount of supernatant and dry it to calculate the concentration of 8%; take 12.5g of the above SF solution, add 0.1L Dilute with water; weigh 1.12g of diammonium hydrogen phosphate and dissolve in SF solution to prepare a phosphorus-silk fibroin (P-SF) solution; weigh 0.11g of silicon orthoacetate solid in 0.2L of purified water, stir and hydrolyze for 3 to 5 hours, then add 3.52g calcium nitrate tetrahydrate, continue to stir for 3h, adjust the pH of the solution to about 7.4, and prepare calcium-silicon solution (Ca-Si); Add dropwise to the P-SF solution at a rate of 2-3ml / min, keep the pH of the system at about 7.4; continue stirring for 3 hours after the addition is complete, keep the pH of the system at about 7.4, and stand at 37°C for 4 hours; After completion, disca...

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Abstract

The invention provides a co-assembled artificial periosteum and a preparation method thereof. The co-assembled artificial periosteum comprises silk fibroin / silicon-doped hydroxyapatite / collagen co-assembled artificial periosteum prepared by co-assembling collagen-mediated silicon-doped hydroxyapatite sol and silk fibroin, and / or the collagen / silicon-doped hydroxyapatite / silk fibroin co-assembled artificial periosteum is prepared by co-assembling silk fibroin-mediated silicon-doped hydroxyapatite sol and collagen. The secondary structure of the silk fibroin is changed through co-assembly of thecollagen and the silk fibroin, so that the co-assembled artificial periosteum has high mechanical property, biocompatibility and ordered and controllable degradability, and the silicon-containing hydroxyapatite has relatively low crystallinity, so that the activity of osteoblasts can be improved, osteogenic differentiation of stem cells is stimulated, and the defect that the silicon-containing hydroxyapatite, the silk fibroin and the collagen are difficult to form and assemble after being blended is overcome.

Description

technical field [0001] The invention relates to the technical field of artificial periosteum, in particular to a co-assembled artificial periosteum and a preparation method thereof. Background technique [0002] The periosteum plays an important role in maintaining the integrity of the bone structure. The periosteum consists of an outer fibrous layer containing collagen, fibroblasts, elastin, blood vessels, and a network of nerves, and an inner cambium containing adult cells that promote bone healing. Mesenchymal progenitor cells, osteoprogenitor cells, osteoblasts and capillaries, etc. In the process of bone repair, the periosteum formed in the healing area plays a very important role in bone repair and reconstruction. The differentiation of mesenchymal stem cells in the periosteum cambium promotes osteogenesis and cortical bone formation, and bone marrow stem cells mainly differentiate into bone marrow bone tissue. At present, clinically, the method of inducing periosteum...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/22A61L27/12A61L27/56A61L27/58
CPCA61L27/24A61L27/227A61L27/12A61L27/56A61L27/58A61L2430/02C08L89/00
Inventor 毛杰周雄杜莹莹王一帆王江林
Owner ASIA BIOMATERIALS WUHAN CO LTD
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