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Method for co-production of 1,3-propanediol and d-1,2,4-butanetriol

A technology of D-1 and propylene glycol, applied in the fields of fermentation and biology, can solve the problems of high price of xylose, low conversion rate, limitation of xylose, etc.

Active Publication Date: 2022-07-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of xylose is high, and the conversion rate of xylose in the production process of 1,3-propanediol is low, which seriously limits the application of xylose in the production of 1,3-propanediol

Method used

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  • Method for co-production of 1,3-propanediol and d-1,2,4-butanetriol
  • Method for co-production of 1,3-propanediol and d-1,2,4-butanetriol
  • Method for co-production of 1,3-propanediol and d-1,2,4-butanetriol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Construction of recombinant Klebsiella pneumoniae

[0050] Specific steps are as follows:

[0051] Synthesize the gene yjhG (SEQ ID No. 6) encoding xylonate dehydratase derived from Escherichia coli; combine the synthesized gene kivD encoding 2-ketoisovalerate decarboxylase with the recombinant plasmid pEtac-kivD-tac -xdh (recorded in the literature "Jing P Y, Cao X, Lu X Y, etc. Modification of an engineered Escherichia coli by a combined strategy of deleting branch pathway, fine-tuning xylose isomerase expression, and substituting decarboxylase to improve1,2,4-butanetriol production[J]. Journal of Bioscience and Bioengineering, 2018, 126(5): 547-552.", the nucleotide sequence of the tac promoter is shown in SEQ ID No. 7) by restriction endonuclease Xba I After digestion with Nco I, ligation was performed to obtain a ligated product; the ligated product was transformed into Escherichiacoli BL21 to obtain a transformed product; the transformed product was co...

Embodiment 2

[0052] Example 2: Co-production of 1,3-propanediol and D-1,2,4-butanetriol

[0053] Specific steps are as follows:

[0054] With Klebsiella pneumoniae (Klebsiella Pneumoniae) JI22914 as a control, the single colony of the recombinant Klebsiella pneumoniae JI22914 / pEtac-yjhG-tac-kivD-tac-xdh obtained in Example 1 was streaked in LB solid medium medium, incubate at 37°C for 12-14h to form a single colony; pick a single colony and inoculate it into LB liquid medium, cultivate at 37°C and 100rpm for 12h to obtain first-class seed liquid; inoculate according to 1% (v / v) Transfer the first-class seed solution to a new LB liquid medium, and cultivate at 37°C and 100 rpm for 8 h to obtain the second-class seed solution; transfer the second-class seed solution to a In the fermentation medium, shake culture at 37° C. and 150 rpm for 48 hours to obtain a fermentation broth.

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Abstract

The invention discloses a method for co-producing 1,3-propanediol and D-1,2,4-butanetriol, belonging to the field of fermentation technology and biotechnology. The invention provides a recombinant Klebsiella that can efficiently transform glycerol and xylose to co-produce 1,3-propanediol and D-1,2,4-butanetriol. The primary bacteria are the host, express the gene xdh encoding xylose dehydrogenase, the gene kivD encoding 2-ketoisovalerate decarboxylase and the gene yjhG encoding xylonate dehydratase; this recombinant Klebsiella is inoculated into After 48 hours of fermentation in the fermentation medium containing glycerol and xylose, the yield of 1,3-propanediol in the fermentation broth can be as high as 18.1g / L, and the yield of D-1,2,4-butanetriol can be as high as 3.3g / L , at this time, the conversion rate of glycerol in the fermentation broth was as high as 45.3%, and the conversion rate of xylose was as high as 12.3%.

Description

technical field [0001] The invention relates to a method for co-producing 1,3-propanediol and D-1,2,4-butanetriol, belonging to the field of fermentation technology and biotechnology. Background technique [0002] 1,3-Propanediol (1,3-propanediol, 1,3-PDO) is a colorless, odorless, viscous liquid with a salty taste and hygroscopicity. In the polyurethane industry, it is often used as the raw material of polyester polyol, the initiator of polyether polyol and the chain extender of polyurethane, etc.; in the organic chemical industry, it is an important monomer and intermediate, the most important The purpose is to synthesize polytrimethylene terephthalate (PTT) as a polymer monomer. Therefore, the market capacity of 1,3-propanediol is huge. [0003] Currently, 1,3-propanediol is usually produced in industry by inoculating Klebsiella Pneumoniae, which can produce 1,3-propanediol, into a fermentation medium containing glycerol for fermentation. However, when Klebsiella pneum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N15/74C12N15/53C12N15/60C12P7/18C12R1/22
CPCC12N9/0006C12N9/88C12N15/74C12P7/18C12Y101/01C12Y401/01071C12Y402/01082
Inventor 诸葛斌刘郁青李玉石陆信曜宗红
Owner JIANGNAN UNIV