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Culture medium for liquid fermentation of pleurotus eryngii

A technology of liquid fermentation and culture medium, which is applied in mushroom cultivation, cultivation, plant cultivation, etc. It can solve the problems of incomplete nutrition, slow germination rate of liquid strains, and susceptibility to infection by miscellaneous bacteria, so as to increase the growth rate and reduce the The effect of miscellaneous bacteria infection rate and good disease resistance

Pending Publication Date: 2020-09-04
贵州贵旺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The nutrition of the Pleurotus eryngii liquid fermentation medium in the prior art is not comprehensive, and the liquid strain made by it has a slow germination rate and is susceptible to miscellaneous bacteria infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The culture medium for liquid fermentation of Pleurotus eryngii, its components are calculated by weight percentage, including soybean powder 8%, soybean meal 12%, white sugar 8%, glucose 6%, potassium dihydrogen phosphate 0.8%, defoamer 0.08%, VB1 1 Tablets / 15L, growth promoter 9%, balance water.

[0022] The preparation method of the culture medium of Pleurotus eryngii liquid fermentation is as follows:

[0023] S1: Add soybean powder, soybean meal, white sugar, glucose, potassium dihydrogen phosphate, VB1 and defoamer into water and stir and mix at 45°C. After mixing evenly, sterilize at 121°C for 60 minutes;

[0024] S2: Adding a growth-promoting agent to the above-mentioned sterilized mixed solution and stirring and mixing to prepare the above-mentioned Pleurotus eryngii liquid fermentation medium.

[0025] The preparation method of above-mentioned growth promoting agent is as follows:

[0026] a): Inoculate the mixed bacterial agent into the sterilized culture m...

Embodiment 2

[0030] The medium for liquid fermentation of Pleurotus eryngii, its components are calculated by weight percentage, including 5% soybean powder, 10% soybean meal, 6% white sugar, 5% glucose, 0.5% potassium dihydrogen phosphate, 0.06% defoamer, VB1 1 Tablets / 15L, growth promoter 10%, balance water.

[0031] The preparation method of Pleurotus eryngii liquid fermentation medium is with embodiment 1.

[0032] The preparation method of growth promoter is as follows:

[0033] a): Inoculate the mixed bacterial agent into the sterilized culture medium according to the inoculum amount of 5%, load the sterile air system, the ventilation rate is 8-10L / min, and cultivate at 33-35°C for 2 days at a constant temperature;

[0034] In step a), the mixed bacterial agent is formed by mixing Bacillus subtilis, Bacillus megaterium, Bacillus amyloliquefaciens and Azotobacter torus in a mass ratio of 5:3:2:6; the effective number of viable bacteria in the mixed bacterial agent is 2.3-2.5×10 9 c...

Embodiment 3

[0037] The culture medium for the liquid fermentation of Pleurotus eryngii, its components are calculated by weight percentage, including soybean powder 6%, soybean meal 11%, white sugar 7%, glucose 6%, potassium dihydrogen phosphate 0.7%, defoamer 0.07%, VB1 1 Tablets / 15L, growth promoter 9%, balance water.

[0038] The preparation method of Pleurotus eryngii liquid fermentation medium is with embodiment 1.

[0039] The preparation method of growth promoter is as follows:

[0040] a): Inoculate the mixed bacterial agent into the sterilized culture medium according to the inoculum amount of 5%, load the sterile air system, the ventilation rate is 8-10L / min, and cultivate at 33-35°C for 2 days at a constant temperature;

[0041] In step a), the mixed bacterial agent is formed by mixing Bacillus subtilis, Bacillus megaterium, Bacillus amyloliquefaciens and Azotobacter torus in a mass ratio of 5:3:2:6; the effective number of viable bacteria in the mixed bacterial agent is 2.2-...

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Abstract

The invention relates to the technical field of plantation of pleurotus eryngii, in particular to a culture medium for liquid fermentation of pleurotus eryngii. The culture medium comprises componentsin percentage by weight as follows: 5%-8% of soybean powder, 10%-12% of soybean meal, 6%-8% of white sugar, 5%-6% of glucose, 0.5%-0.8% of monopotassium phosphate, 0.05%-0.08% of a defoaming agent, 1tablet of VB1 for every 15L, 9%-10% of a growth promoting agent and the balance of water. The culture medium has more comprehensive nutrition through reasonable formula arrangement, so that a pleurotus eryngii liquid strain prepared by the culture medium can effectively increases the growth rate of pleurotus eryngii, the infection rate of infectious microbes in the growth process of pleurotus eryngii can be effectively reduced, and the culture medium is suitable for further popularization and application.

Description

technical field [0001] The invention relates to the technical field of liquid strains of Pleurotus eryngii, in particular to a culture medium for liquid fermentation of Pleurotus eryngii. Background technique [0002] Lentinus genus belongs to Basidiomycetes, Agaricales, Tricholomatacete, and Lentinus. Its scientific name is Lentinusedodes. [0003] The production of traditional shiitake mushrooms generally uses solid strains for inoculation and cultivation. The strains used are mainly conventional solid strains such as sawdust strains and branch strains. This method has a long production cycle and generally takes 55-60 days. Low efficiency; the production process of strains requires a large space, a large amount of manpower, material resources, and high cost; the mycelium culture cycle is long, and the age of mycelia in the medium varies greatly. , the mycelium on the surface of the substrate is close to aging, resulting in irregular fruiting of cultivated species, which c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20
CPCA01G18/20
Inventor 张和贵
Owner 贵州贵旺生物科技有限公司