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A strain of myxobacteria and its application

A technology for myxobacteria and phytopathogenic bacteria, applied in the field of microorganisms, can solve the problems of limited research and application of resistance to phytopathogens, and achieve the effects of being conducive to preservation and transportation, diverse inhibition strategies, and good application value.

Active Publication Date: 2022-01-04
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Myxobacteria can produce a variety of secondary metabolites with novel structures, unique mechanisms of action, and pleiotropic functions. However, most of the current research focuses on metabolites as drug lead compounds, focusing on the anti-phytopathogenic effects of myxobacteria metabolites. There are not many researches and applications

Method used

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  • A strain of myxobacteria and its application
  • A strain of myxobacteria and its application
  • A strain of myxobacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Isolation and purification of bacterial strain

[0035] 1.1 Sample collection and pretreatment

[0036] Collect about 200g of soil in a 10-20cm soil layer from the furnace field of Nanling National Forest Park, remove visible roots, stones and other debris, pass through a 2mm sieve to mix the soil, and spread it at room temperature to dry naturally to reduce subsequent separation and purification Contamination by mold, protists and other miscellaneous bacteria in the process.

[0037] 1.2 Escherichia coli induction method to induce the formation of myxobacteria fruiting bodies

[0038] Take about 10 g of the air-dried soil sample and place it in a 50 mL centrifuge tube, add 15 mL of 100 μg / mL cycloheximide that has been filtered and sterilized, soak overnight, and pour off the liquid after centrifugation. The water agar medium (i.e. WCX medium, containing CaCl 2 2H 2 O 1g / L and agar 15g / L, the balance is water; pH 7.2) isolates phage myxobacteria, draw...

Embodiment 2

[0041] Embodiment 2: the identification of bacterial strain

[0042] 1. Morphological identification of strains

[0043] figure 1 The morphological characteristics of the isolated myxobacteria M34 are shown, among which figure 1 A and figure 1 B is respectively that this bacterial strain is in MD1 liquid culture medium (contains casein peptone 6g / L, soluble starch 2g / L, MgSO 4 .7H 2 O 2g / L and CaCl 2 2H 2 (0.4g / L, the balance is water; pH 7.2) and VY / 2 solid medium after cultivating 5 days aggregation growth characteristics and bacterium colony form, on the VY / 2 plate, show as the light lemon yellow transparent bacterium colony that slides to expand, overall Bacterial colony is moist; Vegetative cells begin to gather and form fruiting body after cultivating 5 days ( figure 1 C and figure 1 D), mature fruiting body is spherical, light lemon yellow, sessile. Crystal violet staining observed under a 100 times oil microscope that the vegetative cells of the strain were lo...

Embodiment 3

[0050] Example 3: Research on anti-phytopathogenic activity of myxobacteria Corallococcus sp.M34 strain

[0051] 3.1 Inhibition of myxobacteria Corallococcus sp.M34 on plant pathogenic fungi

[0052] Pick the rice sheath blight pathogen Rhizoctonia solani Kühn, the banana wilt pathogen Fusarium oxysporum f.sp.cubense race 4, and the banana wilt pathogen Colletotrchum musarum Cooke et Mass Three kinds of phytopathogenic fungal filaments were inoculated on the VY / 2 solid medium at about 2 cm from the edge, and myxobacteria M34 were inoculated at the opposite position on the other side of the plate. Each pathogenic fungus was set for 5 repetitions, and no sticky bacteria was inoculated at the same time. Five bacterial M34 plates were used as controls. Cultivated at 30°C, on the 3rd to 5th day of culture, it was found that it significantly inhibited the growth of Rhizoctonia solani and Discospora banana, while Fusarium oxysporum was less inhibited by Myxobacterium M34 due to its ...

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Abstract

The invention discloses a strain of myxobacteria and its application. The myxobacterium M34 of the present invention has a preservation number of GDMCC No: 61026. The myxobacterium M34 of the present invention can significantly inhibit the growth of plant pathogenic fungi, including Rhizoctonia solani causing rice sheath blight and Fusarium oxysporum causing banana wilt, and Banana spp. Pseudomonas syringae, tomato bacterial wilt pathogen R. solani, cabbage black rot pathogen Xanthomonas campestris, bean wilt pathogen Brachybacterium wilt. The bacterium has a broad-spectrum inhibitory effect on plant pathogenic bacteria, and has various inhibitory strategies, including direct inhibition of fungal growth and predation on bacteria, and has good application value in the preparation of microbial fertilizer additives and biocontrol agents.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a myxobacteria and its application. Background technique [0002] Plant diseases are one of the important limiting factors of crop production, and the annual direct economic losses are estimated to be hundreds of billions of dollars. There are many kinds of plant pathogenic bacteria, and there are many kinds of diseases caused by them, which makes the control difficult. The application of chemical pesticides has made great contributions to the control of crop diseases and insect pests, and the improvement of crop yield and quality. However, the current abuse of pesticides has caused ecological environment damage and threatened human health. In addition, almost every crop has several or even dozens of fungal and bacterial diseases, which leads to poor single chemical control effect; and it is difficult to breed disease-resistant varieties of crops, and the disease resistance i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/20A01P3/00A01P1/00C12R1/01
CPCC05F11/08A01N63/20C12R2001/01C12N1/205
Inventor 周杨朱红惠姚青蚁烁星
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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