26 results about "Haricot beans" patented technology

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a glycyrrhiza uralensis n-butyl alcohol extract and application of effective components thereof. The invention relates to a glycyrrhiza uralensis n-butyl alcohol extract. The effective components of the glycyrrhiza uralensis n-butyl alcohol extract are prepared from glycyrrhiza uralensis isoflavanone, glycyrrhiza stem alcohol A, licorcidine, dibutyl phthalate, glycyrrhizin I, 3 '-Dimethylallylkievitone, lupinone, glycyrrhiza flavonol, edediol, 1-methoxy phaseolin and isoderrone. The glycyrrhiza uralensis n-butyl alcohol extract is prepared from the following raw materials: glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis and glycyrrhiza uralensis, the anti-streptococcus mutans activity of the glycyrrhiza uralensis n-butyl alcohol extract provided by the invention is superior to that of an extract extracted by ethanol in the same extraction method. The method has more advantages in the aspect of preventing streptococcus mutans infection and decayed teeth, and a theoretical basis is provided for research and development of new products.

The invention relates to the field of agricultural microorganisms, and discloses a liquid microbial fertilizer for kidney beans and an application method of the liquid microbial fertilizer, and the liquid microbial fertilizer is composed of a functional microbial strain, a dynamic ion coordination system, a buffer system, a protective agent, nutritional ingredients and a slow-release carrier. The strain is subjected to salt adaptation domestication, so that the salt tolerance is enhanced; the dynamic coordination system relieves salt toxicity through ion coordination and chelation; the buffer system adjusts the pH value of the soil; the slow-release carrier prolongs the action time of the bacterial fertilizer. The bacterial fertilizer can be diluted to be applied to the rhizosphere of the kidney bean or sprayed to the root to improve the saline-alkali soil environment and promote the growth of the kidney bean. The invention solves the problems of poor salt tolerance, short action time, weak soil regulation and control capability and the like of the bacterial fertilizer in the prior art, and is suitable for agricultural production in saline-alkali soil.

The invention provides seed and plants of the bean line SVGW1212. The invention thus relates to the plants, seeds, and tissue cultures of bean line SVGW1212 and to methods for producing a bean plant produced by crossing a plant of bean line SVGW1212 with itself or with another bean plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line SVGW1212, including the pods and gametes of such plants.

The invention provides seed and plants of the bean line SVGG1317. The invention thus relates to the plants, seeds, and tissue cultures of bean line SVGG1317 and to methods for producing a bean plant produced by crossing a plant of bean line SVGG1317 with itself or with another bean plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line SVGG1317, including the pods and gametes of such plants.

The utility model relates to the field of sorting and screening, in particular to an automatic kidney bean sorting and screening all-in-one machine. According to the technical scheme, a movable scraping plate is slidably connected to a fixed sliding rail, a water outlet is formed in the lower end of the movable scraping plate, a sliding rail frame is arranged at the lower end of the water outlet, movable sliding blocks are slidably connected to the sliding rail frame, a movable plate is arranged between the movable sliding blocks, a fixed plate is arranged at the lower end of the movable plate, and a spring guide rod is arranged between the fixed plates; a vibrating motor is arranged at the lower end of the sieve plate, and turning plates are arranged at the front end and the rear end of the sieve plate. The movable scraping plate slides on the fixed sliding rail to scrape kidney beans which are suspended on the water surface due to insufficient weight, so that the kidney beans enter the collecting tank, and screening and distinguishing are facilitated. And after primary screening is completed, the water outlet conducts water drainage work. The movable sliding block slides on the sliding rail frame to drive the movable plate to move, and the kidney beans screened once fall onto the screen plate.

The invention provides an antibacterial peptide as well as application, a product and a preparation method thereof, and relates to the technical field of biology. The invention screens out a novel antibacterial peptide from kidney beans, which can specifically kill escherichia coli O157: H7 without damaging intestinal flora. By designing protective peptide chains at two ends of an effective sequence, an innovative strategy for keeping oral activity without embedding is established, and the stability and the utilization efficiency are remarkably improved. Meanwhile, a plant-based transgenic expression system is constructed, high-purity production of the antibacterial peptide is realized, and the antibacterial peptide can be directly applied to oral administration.

The utility model relates to the field of kidney bean planting auxiliary tools, in particular to a kidney bean cultivation recycling climbing frame which comprises a vertical rod column, a joint head, a transverse rod column and a blade sleeve, the vertical rod column is vertically inserted into soil, the lower end of the joint head is in a sleeve shape, and the upper end of the joint head is in a clamping ring shape. The vertical rod columns are inserted into the upper ends of the joint heads in a penetrating mode and transversely erected between the at least two vertical rod columns, the blade sleeves are installed on the vertical rod columns or the transverse rod columns in a sliding mode, cutting grooves extending in the length direction of the vertical rod columns and the transverse rod columns are formed in the vertical rod columns and the transverse rod columns, and cutting knives of the blade sleeves are embedded into the cutting grooves to move. The frame of the designed structure can be recycled under the condition that the least maintenance work is spent, the pure mechanical structure is adopted, the recycling cost is low, the cutting blade can be replaced at any time, the situation that the cutting blade is not sharp does not exist, environment friendliness and economy are achieved, the climbing frame of multiple building modes has the function of flexible use, and the requirements for small-scale planting and large-scale planting are met. And a large-scale greenhouse type construction planting mode is also met.

The invention discloses a synergistic bactericidal composition and application thereof in prevention and treatment of kidney bean diseases, and belongs to the technical field of pesticides. The active ingredients of the composition comprise citral, azoxystrobin and difenoconazole. Through a specific ratio, the three active ingredients show a remarkable synergistic effect when preventing and treating fusarium, and the problem that the prevention effect of an existing bactericide is reduced due to drug resistance is solved. The composition provided by the invention has the advantages of high efficiency, low toxicity and reduced dosage, and provides a new thought for developing a novel green compound bactericide.

The invention discloses a preparation method and application of a biological medicine, the biological medicine is prepared by compounding alizarin, carrageenan oligosaccharide, agaro-oligosaccharide and a croton vine ethanol extract and taking a multivesicular liposome as a carrier, and through compounding of effective components and matching of the carrier with a slow release effect, the biological medicine can effectively treat kidney bean rust fungi, and has a good application prospect. The composition can effectively kill fungi and stimulate crop resistance, has long-lasting pesticide effect, good pesticide stability, easily degradable components and no residual toxicity, and provides support for development of a novel medicament for preventing and treating the kidney bean rust disease.

This utility model provides a multi-level control-based bean spraying device, specifically belonging to the field of bean cultivation technology. Through the meshing connection of a first bevel gear and a second bevel gear, a control motor can drive a rotating rod to rotate. Through the meshing connection of a push rod and the control gears, the push rod can drive the rotating rod to rotate. The two different control methods allow the device to be used in different situations, improving its ease of use. The adjustable platform allows for simultaneous height adjustment of the two spray heads, enabling the device to spray bean plants growing at the same stage. This utility model includes a pesticide storage tank and an adjustable platform, as well as spray heads, spray nozzles, and a control component. The pesticide storage tank has a storage cavity inside, spray heads are located on both sides of the tank, an adjustable platform is located above the tank, spray heads are located on both sides of the top surface of the platform, and a control component is located in the center of the top surface of the platform.

The utility model relates to the field of kidney bean sowing auxiliary tools, in particular to an anti-blocking kidney bean sowing and soil covering integrated device which comprises a seed storage box, a dibbling wheel disc and a dibbling channel frame, a kidney bean groove is formed in the dibbling wheel disc, a bean outlet is formed in the lower end of the seed storage box, and the kidney bean groove passes through the bean outlet when the dibbling wheel disc rotates. The upper part of the dibbling channel frame is semicircular and surrounds the lower half side of the dibbling wheel disc, the lower part of the dibbling channel frame is provided with a dibbling opening and a ditching moldboard, the ditching moldboard is arranged in front of the dibbling opening, and the dibbling opening is higher than the ditching moldboard. According to the technical scheme, the technical problem that the dibbling opening is easily blocked by soil during planting can be effectively solved, blockage of the dibbling opening can be avoided through cooperation of the dibbling opening and the ditching moldboard, the service life of the machine is ensured, it is ensured that kidney beans are dibbled at equal intervals more smoothly, and use is more convenient.

The invention provides an intelligent kidney bean seed vigor detection method based on hyperspectrum and deep learning, which comprises the following steps: shooting multiple varieties of kidney bean seeds with different vigor grades to obtain original hyperspectral images of the kidney bean seeds; performing image preprocessing on the original hyperspectral image, removing noise through combination of convolution filtering and Gaussian filtering, and extracting an original average spectrum of the kidney bean seeds; extracting a characteristic wavelength in the original average spectrum by using a characteristic wavelength extraction algorithm; and constructing a multi-scale spectrum attention residual error network (MSARN) detection model, dividing an average spectrum into a plurality of scales according to wave bands, extracting multi-scale features through a parallel convolutional network, and outputting final activity grade identification results of different kidney bean seeds after residual error connection and multi-head attention mechanism fusion. The technical problems of insufficient pertinence, limited processing effect, redundant feature extraction, low efficiency, limited model generalization ability and low accuracy of a preprocessing method in the prior art are solved.

This invention discloses a method and its application for creating stress-resistant mutants of common bean using chemical mutagenesis. The main steps include: determining the screening concentration of hydroxyproline (Hyp), determining the median lethal dose of ethyl methanesulfonate (EMS), and subculturing the stress-resistant mutants. After 3-5 subcultures, the stress-resistant mutants screened by this invention ultimately yielded 31 cotyledonary nodes of common bean resistant to hydroxyproline (Hyp), with a mutation rate of 8.16%. This invention can induce mutagenesis and directionally screen for stress-resistant common bean mutants, providing material support for stress-resistant breeding of common bean.

The invention provides seed and plants of the bean line SVGV2138. The invention thus relates to the plants, seeds, and tissue cultures of bean line SVGV2138 and to methods for producing a bean plant produced by crossing a plant of bean line SVGV2138 with itself or with another bean plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line SVGV2138, including the pods and gametes of such plants.

The application discloses a bean weevil population tracing SNP molecular marker and application thereof. Through resequencing of genome samples of different geographical source populations of bean weevils, a SNP marker and corresponding detection primers thereof which can be used for tracing ten populations of the bean weevils, i.e., Guizhou, Yunnan, Ethiopia, Rwanda, Burundi, Cameroon, Congo, Uganda, Angola, Germany and Chile are developed. The population tracing SNP marker site has good genetic stability, and the detection result is reliable. According to a peak diagram after amplification of the detection site amplification primer, the attribution and source of the detected population can be accurately determined, which is helpful to clear the population source of the intercepted bean weevils, and guarantees international agricultural product trade and national ecological and agricultural safety.

The invention discloses a primer group for synchronously detecting seven soybean viruses based on multiple RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and a detection method. The viruses comprise a common kidney bean mosaic virus, a soybean mosaic virus, a light cowpea mottle virus, a soybean green disease virus, a soybean chlorotic leaf roll virus and a pueraria lobata mosaic virus) and a citrus related rhabdovirus. According to the detection method, seven pairs of specific primers (SEQ ID NO.1-14) are included, the cross reaction between the primers is effectively overcome, seven soybean virus fragments can be synchronously amplified in a PCR system, stripes are clear and uniform in distribution, and the detection sensitivity reaches the capacity of independent PCR detection. And after a multiplex PCR amplification system is further optimized, the detection can be economically, quickly and effectively carried out within 2 hours. The method disclosed by the invention has very important significance in monitoring the occurrence condition of soybean virus diseases in the field and predicting disease epidemic.

The invention provides a cultivation method for improving commodity of kidney beans, and belongs to the technical field of kidney bean cultivation. The cultivation method comprises the steps of coating, sowing, flowering period management and podding period management. The flowering phase management step comprises the following steps: when a first inflorescence is formed, topdressing of a kidney bean nutrition fertilizer is carried out, and 32-37 kg of the kidney bean nutrition fertilizer is topdressed per mu; the preparation method of the kidney bean nutrition fertilizer comprises the following steps: mixing potassium sulfate, calcium nitrate, glutamic acid, zinc sulfate and deionized water, uniformly stirring, adding polyvinylpyrrolidone and montmorillonite, uniformly stirring, adding a chitosan solution, stirring for 20-30 minutes, adding sodium alginate, stirring for 27-35 minutes at 40-45 DEG C, adding a calcium chloride solution, and stirring for 4.3-5.0 hours while keeping the temperature, thereby obtaining the kidney bean nutrition fertilizer. The kidney beans cultivated through the method are low in morbidity, high in yield, high in single-pod weight and high in protein and vitamin content.

The present invention provides novel garden bean cultivar Black Diamond and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a bean plant by crossing the bean plants of the invention with themselves or another bean plant. The invention also provides bean plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom.

The utility model provides a seed threshing device for harvesting kidney beans, and particularly belongs to the technical field of kidney bean threshing. Through the structural arrangement that a threaded rod is in threaded connection with a collecting pipe, the threaded rod can drive a threshing blade to adjust the position, so that the threshing blade can cut bean pods of kidney beans, follow-up threshing treatment on the kidney beans is facilitated, damage to the kidney beans is avoided, and the threshing quality of the kidney beans is improved; and the second threshing rods and the first threshing rods are arranged in a staggered structure, so that the threshing effect of the device is improved. The harvester comprises a harvesting box and a threshing box, and further comprises a collecting funnel, a stripping assembly and a threshing assembly. A vibrating module is fixedly mounted on the outer side wall of the collecting pipe; a threshing box is arranged on the outer side below the collecting pipe; a supporting frame is fixedly mounted on the outer side wall of the bottom surface of the threshing box; a stripping assembly is arranged on the inner side of the collecting pipe;

This invention discloses a non-destructive method for detecting protein content in common bean seeds based on hyperspectral imaging, relating to the field of non-destructive testing of agricultural products. It addresses the problems of traditional detection methods being highly destructive, cumbersome, and inefficient, as well as the redundancy, inaccurate feature extraction, and poor model adaptability of existing hyperspectral detection technologies. The invention includes: screening standardized common bean seeds; acquiring hyperspectral data; extracting the average spectrum of the samples using image preprocessing and spectral extraction techniques; eliminating redundant bands using EDA analysis; extracting relevant features using any one of the SFE, PLS-VIP+Pearson+p-value, or KNN-MI schemes; and constructing and selecting the optimal prediction model. This invention can be applied to the screening of high-protein single plants in common bean breeding, automated grading of common bean seed quality, or, after parameter adjustment, to the non-destructive detection of protein content in seeds of other edible legume crops such as peas, chickpeas, and cowpeas, and the tested seeds can be sown normally.

The invention relates to the technical field of molecular marker development, in particular to a KASP molecular marker related to major QTL (quantitative trait loci) of golden water utilization rate of kidney beans, which comprises a first and / or second KASP molecular marker for respectively detecting specific gene region loci of the kidney beans; the method comprises the following steps: during detection, adding a novel fluorescence enhancer FluoroEnhance-X and a primer enhancer Au-PrimerEnhance modified by nanogold into a reaction system; the former enhances a fluorescence signal, and the latter improves the primer performance; in addition, the primer is further subjected to special chemical modification, the 5'end is connected with a cholesterol group, and the 3 'end is introduced with a phosphothio modified group, so that the detection effect is improved. The KASP molecular marker disclosed by the invention can be used for rapidly and accurately detecting the water utilization efficiency in a bean seedling stage; the detection cost is low, the method is not influenced by the environment, and the method can be widely applied to germplasm evaluation, filial generation screening and breeding; the specially designed reaction system and primer modification improve the detection performance, and are of great significance to cultivation of drought-resistant kidney bean varieties.

The application provides a cultivation method for improving commodity quality of kidney beans, and belongs to the technical field of kidney bean cultivation; the cultivation method comprises the steps of coating, sowing, flower period management and pod formation period management; the flower period management step is that when the first inflorescence is opened, kidney bean nutrient fertilizer is applied, and 32-37 kg of the kidney bean nutrient fertilizer is applied per mu; the preparation method of the kidney bean nutrient fertilizer is that potassium sulfate, calcium nitrate, glutamic acid, zinc sulfate and deionized water are mixed, uniformly stirred, then polyvinylpyrrolidone and montmorillonite are uniformly stirred, then a chitosan solution is added, stirred for 20-30 min, sodium alginate is added, stirred for 27-35 min under the condition of 40-45 DEG C, a calcium chloride solution is added, and the mixture is stirred for 4.3-5.0 h, so that the kidney bean nutrient fertilizer is prepared; the kidney bean cultivated by the application has low incidence, high yield, high single pod weight, and high contents of protein and vitamins in the kidney bean.

The application relates to a primer group for detecting a bean golden yellow mosaic virus in Amorphophallus konjac, a kit and a method and application thereof, and belongs to the technical field of biology. The primer group comprises a primer pair TYLCCNV, a primer pair SyLCV and a primer pair TbLCYnV; the bean golden yellow mosaic virus is a golden yellow mosaic virus, a Yunnan tobacco leaf curl virus and a Chinese tomato yellow leaf curl virus. The primer group has the characteristics of high specificity and high sensitivity, and can quickly and efficiently detect the three viruses of TYLCCNV, SyLCV and TbLCYnV from the Amorphophallus konjac.

To provide an electrochemical sensor based on a metal cluster composite material, and its manufacturing method.SOLUTION: The Au25 graphene composite solutions, the kidney bean estrase-chitosan solutions, and the Nafion solutions were dropped on glassy carbon electrodes and dried to obtain electrodes for electrochemical biosensors. The metal clusters consist of 25 gold atoms and a defined number of organic ligands and have a precise structure and a stable geometry. The Au25 graphene composite has excellent conductivity and a cooperative electrocatalytic effect, and can effectively promote the transfer of electrons, thereby improving the response of the sensor. The electrochemical sensor can measure the content of profenofos by using an enzyme inhibition method using kidney bean esterase as an enzyme source.SELECTED DRAWING: Figure 4