Application of rhamnose monosulfate and derivative thereof in resisting skeletal muscle atrophy

A technology of muscle atrophy and sugar derivatives, applied in the field of medicine, to achieve the effects of easy industrialization, promotion of transformation, and inhibition of differentiation

Pending Publication Date: 2020-09-08
QINGDAO CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of rhamnose monosulfate and derivative thereof in resisting skeletal muscle atrophy
  • Application of rhamnose monosulfate and derivative thereof in resisting skeletal muscle atrophy
  • Application of rhamnose monosulfate and derivative thereof in resisting skeletal muscle atrophy

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preparation example Construction

[0040] The preparation method of rhamnotriose monosulfate comprises the steps:

[0041] 1), the green algae reef film or Enteromorpha are made into 0.5-1.5wt% aqueous solution;

[0042] 2), using 0.2-1.0wt% dilute hydrochloric acid to add to the aqueous solution in step 1), heating to 80-100 ° C, and stirring to degrade it for 4-6 hours, and using NaOH to adjust the pH to be neutral;

[0043] 3), the solution obtained in step 2) is concentrated and alcohol-precipitated, and the supernatant is concentrated by a rotator, and then desalted through a gel chromatography column Sephadex G10;

[0044] 4), then use Bio-Gel P4 chromatographic column to separate and purify, the sugar content of the eluent is detected by sulfuric acid phenol method, and the elution curve is drawn;

[0045] 5), collect the peak part, and freeze-dry to obtain rhamnotriose monosulfate.

Embodiment 1

[0048] Prepare the green algae Enteromorpha algae powder into a 1wt% aqueous solution, add 0.5wt% dilute hydrochloric acid to the Enteromorpha aqueous solution, heat to 90°C, stir and degrade for 5 hours, then neutralize it with 0.1M NaOH to pH 7.0, concentrate , alcohol precipitation, the supernatant was concentrated by a rotator, and then desalted through a gel chromatographic column Sephadex G10, and then separated and purified by a Bio-Gel P4 gel chromatographic column, and the sugar content of the eluent was detected by the sulfuric acid phenol method , and draw the elution curve, collect the peak part, and freeze-dry to obtain rhamnotriose monosulfate.

[0049] The obtained rhamnotriose monosulfate structural formula is as follows:

[0050]

[0051] Take an oligosaccharide sample (rhamnotriose monosulfate) and add 0.05mol / l NaBH4 to reflect in the refrigerator at 4°C for 10 hours, then adjust the pH to 7.0 with acetic acid water. The oligosaccharide samples were eluted...

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Abstract

The embodiment of the invention provides an application of rhamnose monosulfate and a derivative thereof in resisting skeletal muscle atrophy. The rhamnose monosulfate or the rhamnose monosulfate derivative is applied to a medicine for resisting skeletal muscle atrophy, or the rhamnose monosulfate or the rhamnose monosulfate derivative is applied to a functional food for resisting skeletal muscleatrophy. The rhamnose monosulfate is used for inhibiting differentiation inhibition of TNF-alpha on skeletal muscle cells C2C12, promoting generation of myosin MHC and myoblast myogenin of C2C12, andpromoting conversion of skeletal muscle myocytes into muscle fibers.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of rhamnotriose monosulfate and derivatives in anti-skeletal muscle atrophy. Background technique [0002] Skeletal muscle atrophy (abbreviated as muscle atrophy) mainly refers to the reduction of skeletal muscle mass and muscle function. The occurrence of muscle atrophy is a comprehensive manifestation of the negative balance of protein degradation and synthesis in skeletal muscle. The influencing factors include changes in related hormones and tissue levels of reactive oxygen species. changes and apoptosis. The common causes of muscle atrophy include long-term malnutrition, systemic or partial limb movement reduction or loss, denervation, etc. Identifying and removing the cause of the disease is an important prerequisite for the treatment and prevention of diseases. Skeletal muscle atrophy caused by long-term malnutrition can be gradually improved ...

Claims

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Application Information

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IPC IPC(8): A61K31/7024C07H11/00C07H1/08A23L33/125A61P21/00A61P19/08
CPCA23V2002/00A61K31/7024A23L33/125A61P19/08A61P21/00C07H1/08C07H11/00A23V2200/316
Inventor 于明圣于淼林
Owner QINGDAO CENT HOSPITAL
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