Tyrosine detection test strip as well as preparation method and application thereof

A technology for detecting test paper and tyrosine, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc., which can solve false positives, limit application value, and reduce detection accuracy, etc. problems, to achieve the effect of high application value, accurate and reliable test results, and not easy to misdiagnose

Active Publication Date: 2020-09-15
苏州良辰生物仪器试剂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some trace components in urine or abnormal metabolic products caused by other causes, such as vitamins, sulfadiazine and other substances, when the content of these substances reaches a certain amount, the test strip will also develop color, which may cause in actual use. The problem of false positives reduces the accuracy of detection and may cause misdiagnosis, limiting its application value

Method used

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  • Tyrosine detection test strip as well as preparation method and application thereof
  • Tyrosine detection test strip as well as preparation method and application thereof
  • Tyrosine detection test strip as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The tyrosine detection test strip provided in this embodiment includes filter paper and tyrosinase, sucrose, bovine serum albumin, Triton X-100, TMB and carbamide peroxide loaded on the filter paper.

[0032] Specifically, it is prepared by the following method:

[0033] (1) Weigh 2.137g sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 2H 2 O) and 2.26g disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 12H 2 O) mixing and adding water to 80mL to obtain a phosphate buffer;

[0034] (2) Weigh 300KU tyrosinase dry powder and add it to the buffer in step (1);

[0035] (3) Then add 2mL TMB;

[0036] (4) Then add 1.5g bovine serum albumin, 2.5g sucrose and 1.5g Triton X-100 to obtain a mixed solution,

[0037] (5) Then add 2mg of carbamide peroxide to prepare a mixed solution, add purified water to make up to 100mL. In the mixed solution, the concentration of sodium dihydrogen phosphate monohydrate is 2.137g / mL, the concentration of disodium hydrogen phosphate...

Embodiment 2

[0040] The tyrosine detection test strip provided in this example is different from Example 1 in that the concentration of carbamide peroxide in the mixed solution is 0.04 mg / mL.

Embodiment 3

[0041] Embodiment 3 effect verification test

[0042] 2.1 Discoloration test

[0043] A, immerse the tyrosine detection test strip prepared according to Example 1 and the p-hydroxyphenylalanine test strip of CN108872222A of the comparative patent in the test sample and take it out, the discoloration result is as follows figure 1 Shown, among them, the concentration of test sample is the tyramine of 0mg / L, 50mg / L, 100 mg / L, 150 mg / L, 200 mg / L, 250 mg / L, 324 mg / L, 500 mg / L acid standard solution.

[0044] pass figure 1It can be seen that when the test sample is purified water (that is, the concentration of the test sample is 0), the test paper strip of embodiment 1 and the test paper strip of the comparative patent are all white, and when the concentration of the test sample is 50 mg / L, the implementation The test paper strip of example 1 and the test paper strip of comparative patent all began to change color, and when the concentration of test sample was 100mg / L, the test p...

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Abstract

The invention relates to a tyrosine detection test strip as well as a preparation method and application thereof. The tyrosine detection test strip comprises filter paper, tyrosinase, sucrose, albumin, Triton X-100, 3, 3', 5, 5'-tetramethyl benzidine and urea peroxide, wherein the tyrosinase, the sucrose, the albumin, the Triton X-100, the 3, 3', 5, 5'-tetramethyl benzidine and the urea peroxide are loaded on the filter paper. According to the tyrosine detection test strip disclosed by the invention, the false positive problem of the existing test strip can be solved by the urea peroxide bleaching effect by adding the urea peroxide load on the filter paper, so that the test strip disclosed by the invention is not easily interfered by vitamins and sulfonamides, the detection result is moreaccurate and reliable, misdiagnosis is not easily caused, and the application value is high. The tyrosine detection test strip can be used for detecting the tyrosine content in urine; when the urine contains tyrosine, the tyrosine can be detected through test strip color development; and the tyrosine concentrations are different, and the color development results are different.

Description

technical field [0001] The invention belongs to the technical field of detection reagents, and in particular relates to a tyrosine detection test strip and a preparation method and application thereof. Background technique [0002] Early diagnosis and early treatment of tumors are the key to improving the cure rate of tumors. The commonly used diagnostic methods in clinical practice include chest X-ray, B-ultrasound, CT, and nuclear magnetic resonance, etc., which are often accompanied by puncture, blood drawing, etc., which aggravate the pain of the patient and may even cause cross-infection, and are expensive. More importantly, through these The tumors that can be detected by means are generally in the middle and late stage, which brings great pain and economic burden to the patients. Therefore, it is imperative to develop a detection method with simple and quick operation, low cost, high sensitivity and good repeatability. [0003] Abnormal nucleotide metabolism of canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 王继良卢锦超
Owner 苏州良辰生物仪器试剂有限公司
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