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Method for demulsifying and extracting grease from oil-containing algae or fungal cell wall-broken liquid

A fungal cell and wall-breaking liquid technology, which is applied in the direction of fat oil/fat production and fat production, can solve the problems of narrow application range, long production time, and large amount of organic solvents, so as to improve product benefits, high comprehensive utilization rate, The effect of improving the oil extraction rate

Pending Publication Date: 2020-09-22
XIAMEN HUISON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When organic solvents are used for demulsification, the amount of organic solvents is large, and the crude oil has complex components. At the same time, heating the crude oil during the desolventization process will easily cause the quality of the crude oil to deteriorate, especially the peroxide value and anisidine value in the oil. It will cause organic solvent residue, which affects production and food safety; when using microwave synergistic demulsification, the energy consumption is high, the process is complicated, the production time is long, and the quality of the product is poor; when surfactant is used for demulsification, the crude oil has complex components, Narrow scope of application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. The wall-breaking solution refers to the production of step 4 of the invention content of the patent "A method for extracting DHA from algae and fungal cells by breaking the wall ZL200910225296.6" "Bacterial slime is subjected to high-pressure homogeneous wall breaking through a high-pressure homogenizer" The obtained wall-breaking solution;

[0044]2. 70000 kg of wall-breaking emulsion was diluted with deionized water to a solid content of 15% according to the solid content requirements, and the antioxidant D-sodium erythorbate and ascorbyl palmitate were added (the mass ratio of the two antioxidants was 5:1 ) 21kg, the temperature of the broken tank is raised to 70°C and maintained for 2 hours;

[0045] 3. Vacuumize the broken tank to -0.08MPa, add 0.5% magnesium chloride and calcium chloride of the volume of the broken emulsion before dilution (according to 1:1 ratio, the mass concentration of magnesium chloride and calcium chloride solution is 30%), control Temp...

Embodiment 2

[0052] 1. 60,000kg of the wall-breaking emulsion is diluted with deionized water to a solid content of 22% according to the solid content requirements, and the antioxidant D-sodium erythorbate and ascorbyl palmitate are added (the mass ratio of the two antioxidants is 5:1 ) 12kg, the temperature of the broken tank is raised to 80°C and maintained for 2 hours;

[0053] 2. Vacuumize the broken wall tank to -0.06MPa, add 2.0% sodium sulfate and magnesium sulfate of the volume of the broken wall emulsion before dilution (according to 1:1 ratio, the mass concentration of sodium sulfate and magnesium sulfate solution is 50%), control Temperature 78-80°C, stirring speed 30rpm and nitrogen tumbling for 1h;

[0054] 3. After the reaction is complete, add 0.5% malic acid (the mass concentration of malic acid is 50%) of the volume of the wall-breaking emulsion before dilution, stop stirring immediately after mixing, and centrifuge after standing for 1 hour. Centrifuge in a three-phase c...

Embodiment 3

[0058] 1. 50,000kg of the wall-breaking emulsion is diluted with deionized water to a solid content of 12% according to the requirements of the solid content, and the antioxidant D-sodium erythorbate and ascorbyl palmitate are added (the mass ratio of the two antioxidants is 5:1 )

[0059] 10kg, the temperature of the broken tank is raised to 90°C and maintained for 1 hour;

[0060] 2. Vacuumize the broken wall tank to -0.1MPa, add 2.0% solution of sodium sulfate and EDTA-2Na of the volume of the broken wall emulsion before dilution (according to the ratio of 1:1, the mass concentration of sodium sulfate and EDTA-2Na solution is 40 %), control temperature 88-90 ℃, stirring speed 30rpm and nitrogen tumbling 1h;

[0061] 3. After the reaction is complete, add 2.0% malic acid (the mass concentration of malic acid is 50%) of the volume of the wall-breaking emulsion before dilution, stop stirring immediately after mixing, centrifuge after standing for 1 hour, and then centrifuge a...

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Abstract

The invention discloses a method for demulsifying and extracting grease from oil-containing algae or fungal cell wall-broken liquid. The pH of the wall-broken liquid is adjusted under the vacuum condition, a proper amount of inorganic salt is added, the stirring rotating speed and temperature in different time periods are regulated and controlled, oil, water, protein and fermentation extracellularproducts in the wall-broken liquid are completely separated, and the extraction yield of intracellular grease of algae and fungal cells is increased to 90%-96% from 85%-90%. When the method is used for demulsifying oil-containing emulsion, solvent residues caused by extraction and demulsification with an organic solvent and unsaturated fatty acid oxidation caused by high-temperature solvent recovery can be effectively avoided, meanwhile, various physicochemical indexes of the extracted crude grease are better, the quality of the crude grease is better, and the green, non-toxic and residue-free effects in the product extraction process are achieved.

Description

technical field [0001] The invention relates to the fields of industrial microbial fermentation and food and feed, in particular to a method for demulsifying and extracting oil from oil-containing algae or fungal cell wall breaking liquid. Background technique [0002] DHA belongs to the ω-3 series of polyunsaturated fatty acids (ω-3PUFAs). It is a main substance for the growth and maintenance of nervous system cells. It is also an important component of the brain and retina. Nutrients necessary for the development of the retina are beneficial to the extension and regeneration of brain cell processes, which can enhance memory and thinking and improve intelligence, so it has the reputation of "brain gold". DHA is an essential fatty acid that is difficult for the human body to synthesize. Its content is as high as 20% in the cerebral cortex and about 50% in the retina. It is very important for the intelligence and vision development of fetuses. Deficiency will lead to growth r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
CPCC11B1/10C11B1/025C11B1/04
Inventor 陈礼毅钟惠昌陈水荣李伟杰
Owner XIAMEN HUISON BIOTECH
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