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Preparation based on polypyrrole-iron phosphate-oxidase nanoparticles and applications

A nanoparticle and polypyrrole technology, which is applied in the direction of oxidoreductase, immobilized enzyme, biochemical equipment and methods, etc., can solve the problems of no research reports on polypyrrole-iron phosphate-oxidase composite nanoparticles, etc., and achieve good results Effects of in vitro performance, simple preparation process, and easy operation

Pending Publication Date: 2020-09-29
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The literature and patent results of searching domestic and foreign related polypyrrole nanoparticles for cancer treatment found that: before the completion of the present invention, the preparation of polypyrrole-iron phosphate-oxidase composite nanoparticles based on polyvinyl alcohol stabilization and its Research Reports on Combination Therapy of Cancer Cells with Light, Heat and Starvation

Method used

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  • Preparation based on polypyrrole-iron phosphate-oxidase nanoparticles and applications
  • Preparation based on polypyrrole-iron phosphate-oxidase nanoparticles and applications
  • Preparation based on polypyrrole-iron phosphate-oxidase nanoparticles and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Dissolve 241.0 mg of poly(vinyl alcohol) (PVA) in 6.025 mL of water, heat and stir it magnetically for 10 min to mix well, and cool for 10 min. Join FeCl 3 ·6H 2 O (90.2 mg / mL) 13.919 mL was stirred and reacted for 1 h. Under ice bath conditions, 139.5μL of pyrrole monomer was added, and oxidative polymerization was carried out for 4 h. The polypyrrole nanoparticles obtained after centrifugal purification were stored at 4°C for later use.

[0033] The TEM image shows the size and morphology of the polypyrrole nanoparticles. Refer to the instructions attached figure 1 . The TEM image shows that the polypyrrole nanoparticles were dispersed in phosphate buffer to grow iron phosphate and then loaded with glucose oxidase ( figure 1 a. figure 1 b) It can be seen that small spheres grow on the big spheres, indicating that the iron phosphate has successfully grown on the polypyrrole nanoparticles.

Embodiment 2

[0035] Take 40.0 mg of Polypyrrole (PPy), dissolve it in 31 mL of ultrapure water, add 4.0 mL of phosphate buffer (0.1mol / L), shake with a shaker for 4 hours, and mix well. After the reaction, 2 mL (10 mg / mL) of glucose oxidase solution was directly added to the resulting solution, and the polypyrrole-iron phosphate-glucose oxidase (PPy-FePi-GOX) nanoparticles were obtained after centrifugal purification, and stored at 4°C.

[0036] The laser particle size analyzer (DLS) test results show the hydrodynamic size and uniformity of the obtained composite nanoparticles. Refer to the instructions attached figure 2 . figure 2 a shows that the average size of PPy nanoparticles is 168.4 ± 8.7 nm, and the monodispersity is 0.203 ± 0.042. figure 2 b shows that the average size of PPy-FePi-GOX composite nanoparticles is 232.1 ± 21.8 nm, and the monodispersity is 0.232 ± 0.014.

Embodiment 3

[0038] Take 0.5 mL (3 mg / mL) of PPy-FePi-GOX nanoparticles prepared in Example 2, and set the final reaction concentration of glucose to 50, 100, 200, 400, and 600 ppm, respectively. Use a pH meter to test the acid strength generated during the reaction, and set the time to 0, 0.5, 1, 2, 3, 4, 5, 6 h. The test result is like image 3 It is shown that GOX consumes glucose to produce gluconic acid during this reaction. The pH of the solution decreases with the prolongation of the reaction time and is positively correlated with the glucose concentration.

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Abstract

The invention relates to preparation based on polypyrrole-iron phosphate-oxidase nanoparticles and applications. The preparation includes as follows: taking polyving akohol as a stabilizing agent andferric trichloride as an oxidizing agent to prepare polypyrrole nanoparticles; dispersing the polypyrrole nanoparticles in phosphate buffer to in-situ grow iron phosphate nanoparticles; performing loading on glucose oxidase; and performing in vitro performance evaluation on an obtained final product. The method is simple and easy to operate; and the prepared polypyrrole-iron phosphate-oxidase nanoparticles have good stability and versatility.

Description

Technical field [0001] The invention belongs to the field of preparation of cancer cell therapeutic agents, and particularly relates to the preparation and application of polypyrrole-ferric phosphate-oxidase nanoparticles. Background technique [0002] Tumors are the number one killer that endangers human life and health. In recent years, tumors have shown an obvious upward trend, and the mortality rate is extremely high. According to recent surveys, it has been proved that cancer treatment based on a single method is not enough and inefficient. The development of collaborative treatments is attractive and has attracted huge attention. Compared with single treatment, combination therapy can reduce drug resistance and synergistically inhibit the development of cancer, thereby improving the efficacy of cancer. The increase in the penetration depth of the nanocarrier is beneficial to improve the therapeutic effect by penetrating the material into the tumor. This can be through va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K38/44A61K9/14A61K47/32A61K47/02A61P35/00C12N11/089
CPCA61K9/143A61K9/146A61K38/443A61K41/0052A61P35/00C12N9/0006C12Y101/03004C12N11/089A61K2300/00
Inventor 刘辉徐路恩
Owner SOUTHWEST UNIV
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