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Preparation method of sandwich-type photoelectrochemical sensor for detecting procalcitonin by fullerene-tritin tetroxide

A technology of tritin tetroxide and procalcitonin, applied in tin oxide, fullerene, scientific instruments, etc.

Active Publication Date: 2022-07-01
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of tritin tetroxide materials in photoelectrochemical sensors has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation of Photoelectrochemical Sensor

[0049] (1) Preparation of flower-like tritin tetroxide material

[0050] Dissolve 1.0 g of stannous chloride dihydrate and 3.0 g of trisodium citrate dihydrate together in 10 mL of ultrapure water, stir and mix the solution at room temperature, and take it as solution A; take 0.25 g of sodium hydroxide to dissolve In 10 mL of ultrapure water, stir evenly at room temperature as solution B; mix solution A and solution B, stir at room temperature for 18 h, transfer the mixed solution into an autoclave, and carry out at 150 ° C. The hydrothermal reaction was carried out for 10 h, and after the reaction was finished, it was naturally cooled, and the product was washed 5 times with ultrapure water, and dried at 30 ° C for 10 h to obtain flower-shaped tin tetroxide material;

[0051] (2) Preparation of fullerene quantum dots

[0052] Dissolve 1 mg of fullerene powder in 1 mL of anhydrous toluene solvent, stir evenly at r...

Embodiment 2

[0071] Example 2 Preparation of photoelectrochemical sensor

[0072](1) Preparation of flower-like tritin tetroxide material

[0073] Dissolve 1.3 g of stannous chloride dihydrate and 3.2 g of trisodium citrate dihydrate together in 14 mL of ultrapure water. After stirring and mixing the solution at room temperature, it was taken as solution A; In 14 mL of ultrapure water, stir evenly at room temperature as solution B; mix solution A and solution B, stir at room temperature for 20 h, transfer the mixed solution into an autoclave, and carry out at 160 ° C. The hydrothermal reaction was carried out for 12 h, and after the reaction was finished, it was naturally cooled, and the product was washed with ultrapure water for 5 times, and dried at 40 ° C for 12 h to obtain the flower-shaped tin tetroxide material;

[0074] (2) Preparation of fullerene quantum dots

[0075] Dissolve 3 mg of fullerene powder in 3 mL of anhydrous toluene solvent, stir evenly at room temperature to obta...

Embodiment 3

[0094] Example 3 Preparation of Photoelectrochemical Sensor

[0095] (1) Preparation of flower-like tritin tetroxide material

[0096] Dissolve 1.5 g of stannous chloride dihydrate and 3.5 g of trisodium citrate dihydrate together in 15 mL of ultrapure water, and stir and mix the solution at room temperature as solution A; take 0.35 g of sodium hydroxide to dissolve In 15 mL of ultrapure water, stir evenly at room temperature as solution B; mix solution A and solution B, stir at room temperature for 24 h, transfer the mixed solution into an autoclave, and carry out at 200 ° C. The hydrothermal reaction was carried out for 16 h, and after the reaction was finished, it was naturally cooled, and the product was washed 5 times with ultrapure water, and dried at 60 ° C for 14 h to obtain flower-shaped tin tetroxide material;

[0097] (2) Preparation of fullerene quantum dots

[0098] Dissolve 5 mg of fullerene powder in 5 mL of anhydrous toluene solvent, stir evenly at room tempe...

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Abstract

The invention relates to a preparation method of a sandwich-type photoelectrochemical sensor for detecting procalcitonin based on a fullerene-tritin tetroxide. The invention adopts the tritin tetroxide composite material sensitized by fullerene quantum dots and cadmium selenide as the base photosensitive material, and the fullerene has excellent electron transfer efficiency, which can effectively enhance the photocurrent response of the tin oxide material. Second, the carboxyl-functionalized cadmium selenide can effectively link biomolecules, and at the same time act as a sensitizer to further promote the basic photocurrent response and improve the stability of the sensor. Ferric disulfide has good light absorption properties, and it is used as a marker to label the second antibody of procalcitonin to construct a sandwich-type sensor, which can effectively improve the sensitivity of the sensor and realize the ultrasensitive detection of procalcitonin. Its detection limit is 3.5 fg / mL.

Description

technical field [0001] The invention relates to a preparation method of a sandwich type photoelectrochemical sensor for detecting procalcitonin based on a fullerene-tritin tetroxide. Specifically, a sandwich-type optoelectronic device for procalcitonin detection was prepared by using tin tetroxide co-sensitized with fullerene quantum dots and cadmium selenide as the base photosensitive material, and using iron disulfide as a marker to label the secondary antibody. The chemical sensor belongs to the technical field of novel functional materials and biological sensing detection. Background technique [0002] Procalcitonin (PCT) is a protein that increases in plasma levels during severe bacterial, fungal, and parasitic infections, as well as in sepsis and multiple organ failure, and bacterial endotoxin plays a role in the induction process. Crucial role, local limited bacterial infection, mild infection and chronic inflammation will not lead to its increase, and PCT will not i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/154C01G19/02C01G49/12G01N27/327G01N27/416B82Y30/00B82Y40/00
CPCC01B32/154C01G19/02C01G49/12G01N27/416G01N27/3278B82Y30/00B82Y40/00C01P2004/64C01P2004/30Y02P20/133
Inventor 魏琴徐芮王欢任祥范大伟
Owner UNIV OF JINAN