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Chitosan hydrogel and preparation method thereof

A technology of chitosan and sugar water, which is applied in the field of medicine, can solve the problems of uncontrollable drug release, difficulty in injecting drug administration, and large hydrogel consistency, and achieve the effects of preventing sudden drug release, low cost, and simple preparation method

Pending Publication Date: 2020-10-09
瑞希(重庆)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chitosan hydrogel has heat-sensitive properties under the condition of high concentration, but the hydrogel has a large consistency, is not easy to inject, and has the disadvantage of sudden release, which cannot control the release of the drug at a uniform rate, which may cause certain side effects. Hidden dangers of medication

Method used

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  • Chitosan hydrogel and preparation method thereof
  • Chitosan hydrogel and preparation method thereof
  • Chitosan hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of chitosan hydrogel, by weight percentage, this chitosan hydrogel comprises following components: the chitosan of 0.4% tetrazine amine modification, the chitosan of 0.1% norbornene modification, active ingredient (interference element) 1%, acceptable adjuvant (preservative [potassium sorbate] 1%, stabilizer [glycerin] 3%) 4% in the external preparation for skin, and the balance is purified water. This chitosan hydrogel is prepared as follows:

[0040] (1) Add chitosan and bromoacetic acid with a molecular weight of 10k to a NaOH solution with a concentration of 1M, and react for 7 hours at 2°C to obtain carboxychitosan, wherein the mass ratio of chitosan and bromoacetic acid is 1 :3;

[0041](2) The carboxyl chitosan prepared in step (1) is dissolved in the MES buffer solution with a concentration of 0.1M, and 4-(4,6-dimethoxytriazine)-4-methylmorpholine is added Hydrochloride, with 100rpm speed stirring reaction 1h, obtain the solution containing activated ca...

Embodiment 2

[0047] A kind of chitosan hydrogel, by weight percentage, this chitosan hydrogel comprises following components: the chitosan of 6% tetrazine amine modification, the chitosan of 1% norbornene modification, active ingredient (collagen ) 10%, acceptable adjuvants (preservative [chlorocresol] 1%, stabilizer [glycerin] 10%) 11% in external skin preparations, and the balance is purified water. This chitosan hydrogel is prepared as follows:

[0048] (1) Add chitosan and bromoacetic acid with a molecular weight of 15k to a NaOH solution with a concentration of 1M, and react for 6 hours at 4°C to obtain carboxy chitosan, wherein the mass ratio of chitosan and bromoacetic acid is 1 :4;

[0049] (2) The carboxyl chitosan prepared in step (1) is dissolved in the MES buffer solution with a concentration of 0.1M, and 4-(4,6-dimethoxytriazine)-4-methylmorpholine is added Hydrochloride, with 300rpm speed stirring reaction 1h, obtain the solution containing activated carboxy chitosan, where...

Embodiment 3

[0055] A kind of chitosan hydrogel, by weight percentage, this chitosan hydrogel comprises following components: the chitosan modified by 1% tetrazine amine, the chitosan modified by 0.5% norbornene, active ingredient (light propene) 1%, acceptable excipients (preservative [chlorocresol] 0.01%, stabilizer [cyclodextrin] 0.01%) 0.02% in external skin preparations, and the balance is purified water. This chitosan hydrogel is prepared as follows:

[0056] (1) Add chitosan and bromoacetic acid with a molecular weight of 20k to a NaOH solution with a concentration of 1M, and react for 8 hours at 0°C to obtain carboxychitosan, wherein the mass ratio of chitosan and bromoacetic acid is 1 :5;

[0057] (2) The carboxyl chitosan prepared in step (1) is dissolved in the MES buffer solution with a concentration of 0.1M, and 4-(4,6-dimethoxytriazine)-4-methylmorpholine is added Hydrochloride, with the speed stirring reaction of 500rpm 0.5h, obtain the solution containing activated carbox...

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Abstract

The invention relates to chitosan hydrogel and a preparation method thereof, which belong to the technical field of medicines. The hydrogel comprises chitosan modified by tetrazinamine, chitosan modified by norbornene and the balance of water, and further comprises active ingredients and auxiliary materials. When the hydrogel is prepared, tetrazinamine modified chitosan and norbornene modified chitosan are used as raw materials; a rapid and efficient Diels-Alder reaction is carried out on a tetrazine functional group and norbornadiene; through the reaction, the active substance can be effectively encapsulated, the release degree of the active substance is controlled while the activity of the active substance is better maintained, and the active substance is slowly released at a certain speed within a specified time range according to the designed dosage. Besides, the hydrogel also contains disulfide bonds, and the disulfide bonds can be biologically degraded in the presence of GSH, sothat the gradual degradation of the gel is promoted, a medicine is stably released, and the sudden release of the medicine is further prevented. The preparation method of the hydrogel is simple, easyto operate, low in cost and suitable for expanded production.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a chitosan hydrogel and a preparation method thereof. Background technique [0002] Due to the development of molecular biology and cell biology, a large number of protein and peptide drugs and vaccines have been approved or are undergoing clinical trials, but their poor stability, easy degradation in the gastrointestinal tract, and short half-life will lead to a significant decline in drug efficacy , Biodegradable materials control the release of proteins, use their characteristics to embed in subcutaneous or tissue, continuously release active ingredients, target drug delivery, prolong half-life and increase protein stability. [0003] However, since the efficacy of protein drugs is closely related to the three-dimensional structure of their molecules, the integrity of the structure must be guaranteed during the entire formulation process in the protein delivery sy...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/36A61K45/00
CPCA61K9/0019A61K9/06A61K47/36A61K45/00
Inventor 冯文学李杨洪
Owner 瑞希(重庆)生物科技有限公司