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3D multi-layer high-flux organ chip and preparation method and application thereof

An organ-on-a-chip, high-throughput technology, applied in biochemical equipment and methods, biochemical instruments, bioreactor/fermenter combinations, etc., to achieve the effects of high-throughput operation and characterization, simple methods, and simple preparation methods

Pending Publication Date: 2020-10-09
BEIJING DAXIANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organ chip technology has successfully constructed a variety of in vitro models for drug screening, but there are few reports on the establishment of high-throughput biological barriers and simultaneous evaluation of drug anti-glioma activity

Method used

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  • 3D multi-layer high-flux organ chip and preparation method and application thereof
  • 3D multi-layer high-flux organ chip and preparation method and application thereof
  • 3D multi-layer high-flux organ chip and preparation method and application thereof

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preparation example Construction

[0105] In the second aspect of the embodiments of the present invention, a method for preparing a 3D multilayer high-throughput organ chip includes the following steps:

[0106] S11, washing the liquid storage layer 10, the film layer 20 and the 3D culture layer 30 respectively, and then drying;

[0107] S12, using liquid PDMS glue to sequentially stack and bond the film layer and the 3D culture layer to one side of the liquid storage layer to obtain a prefabricated three-layer chip;

[0108] S13. Dry the prefabricated three-layer chip to obtain a three-layer chip; complete the preparation of a 3D multi-layer high-throughput organ chip to obtain a 3D multi-layer high-throughput organ chip.

[0109] According to the preparation method of the embodiment of the present invention, the prepared 3D multilayer high-throughput organ chip has three layers, including a liquid storage layer 10 , a thin film layer 20 and a 3D culture layer 30 that are sequentially stacked and connected (b...

Embodiment 1

[0151] Example 1 3D multilayer high-throughput organ chip I

[0152] Such as Figure 1 to Figure 2 As shown, the 3D multi-layer high-throughput organ chip I includes a liquid storage layer 10 , a film layer 20 and a 3D culture layer 30 that are stacked and connected in sequence.

[0153] Wherein, the liquid storage layer 10 is a liquid storage plate with holes (such as a PMMA plate), and the liquid storage through hole 11 is a through hole opened on the body of the liquid storage plate. The liquid storage through hole 11 is a circular straight hole (cylindrical hole) with a diameter of 6 mm and a depth of 3 mm.

[0154] The film layer 20 adopts a polycarbonate film (PC film) with micropores 21 and a pore diameter of 0.4 μm.

[0155] The 3D culture layer 30 adopts a culture plate with holes (eg, PMMA plate), and the culture microwells 31 are through holes opened on the body of the culture plate. The culture microwell 31 is a circular straight hole (cylindrical hole) with a d...

Embodiment 2

[0157] Example 2 3D multilayer high-throughput organ chip II

[0158] Such as Figure 3 to Figure 4 As shown, the 3D multi-layer high-throughput organ chip II includes a liquid storage layer 10 , a film layer 20 and a 3D culture layer 30 that are sequentially stacked and connected.

[0159] Wherein, the surface of the liquid storage layer 10 has a plurality of protrusions protruding from the surface (ie, the cylinder portion 110 ), and through holes are opened on the protrusions to form a plurality of liquid storage cylinder holes 11 . A frame 102 is formed on the liquid storage layer around the through-hole liquid storage area (composed of a plurality of liquid storage column holes 11 ) to form a liquid storage tank 100 . Moreover, the liquid storage column holes 11 are located in the liquid storage tank 100 . That is, the liquid storage layer 10 is a tank plate (for example, made of PMMA) with liquid storage column holes. Wherein, a gap 101 is provided between the liquid ...

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Abstract

The invention discloses a 3D multi-layer high-flux organ chip as well as a preparation method and an application thereof, and belongs to the field of biological tissue engineering. The chip comprisesa liquid storage layer, a thin film layer and a 3D culture layer which are stacked in sequence. The method is characterized by completing bonding connection of the liquid storage layer, the thin filmlayer and the 3D culture layer by utilizing PDMS glue, and constructing a biological barrier cell layer on the thin film layer on the liquid storage layer side of the chip, and carrying out 3D cell culture in the culture micropores of the 3D culture layer of the chip to construct the biological barrier model. The chip can be used for researching interaction of two cells, angiogenesis, drug permeation, cell polarization, cell migration, drug activity evaluation and the like. Moreover, an in-vitro model and a biological barrier model which are combined with high-throughput drug screening can beconstructed, and the method is used for screening of anti-brain glioma compounds of blood-brain barriers and related research. Moreover, the liquid is convenient to operate and suitable for high flux,and external equipment is not needed. The construction method is simple and effective.

Description

technical field [0001] The invention relates to the technical field of biological tissue engineering, in particular to a 3D multilayer high-throughput organ chip and its preparation method and application. Background technique [0002] Cancer is a disease caused by the loss of normal regulation and excessive proliferation of body cells. Cancer cells can invade surrounding tissues (invasion) and spread to other parts of the body through the circulatory system and / or lymphatic system. There are various types of cancer, and the severity of the disease depends on the location of the cancer cells, the degree of malignancy and whether they have metastasized. Glioma is different from other tumors in that its tumor cells grow and invade normal tissues very quickly, and can damage all tissues at the metastatic site. At the same time, it is difficult to know the exact location of the tumor due to the irregular shape of the brain. One of the main factors affecting the development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M3/00
CPCC12M23/02
Inventor 肖荣荣周宇
Owner BEIJING DAXIANG BIOTECH CO LTD
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