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Artificial inoculation identification method for stalk rot resistance of corn varieties

A technology of stem base rot and artificial inoculation, applied in the agricultural field, can solve the problems of difficult control of inoculation amount, unsuitable for identification of large-scale resistant materials, poor stability of inoculation effect, etc.

Active Publication Date: 2020-10-09
NORTHEAST AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these methods, the most widely used artificial inoculation method is the root burial method, and the root burial method has a low incidence rate, is greatly affected by the environment, has poor stability of the inoculation effect, and the preparation of the inoculum and the inoculation process are complicated and the workload is large.
There is also the toothpick method, which has a large workload in the preparation stage and is not easy to control the amount of inoculation, so it is not suitable for the identification of a large number of corn resistant resources
The traditional injection method that has been reported is to inoculate with a common syringe. The inoculation period is after the corn is growing. After the rhizome is punched with an electric drill, the bacterial suspension is injected into the stem with a syringe, and the inoculation site is blocked with vaseline. This method The workload is heavy, and it is not easy to investigate the later onset
At present, the reported inoculation methods are not suitable for the identification of large quantities of resistant materials.

Method used

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  • Artificial inoculation identification method for stalk rot resistance of corn varieties
  • Artificial inoculation identification method for stalk rot resistance of corn varieties
  • Artificial inoculation identification method for stalk rot resistance of corn varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Isolation and Identification of Maize Stem Root Rot Pathogen

[0037] 1. Collection of corn stalk rot samples

[0038] Corn stover samples were collected at 15 sites in 6 prefecture-level cities in Heilongjiang Province, China, showing light brown lesions in the lower part of the stem nodes and dark brown pith tissue or disintegration of the stem pith. More than 10 disease samples were collected from each site and brought back to the laboratory for isolation.

[0039] 2. Make culture medium

[0040] Potato agar (PDA) medium: Take 200g peeled potato pieces, cut into small pieces, add less than 1000mL of water to boil for 30min, filter with double gauze, make up 1000mL with distilled water, add 20g of glucose, a small amount of multiple additions for a total of 18g Agar powder, stirred continuously, divided into test tubes and Erlenmeyer flasks, and then placed in a heat sterilizer at 121°C for 20 minutes to sterilize, and set aside.

[0041] Carnation leaf (CLA) cultu...

Embodiment 2

[0069] Resistance Identification of Maize Varieties to Stem Rot

[0070] 1. Preparation of Inoculum

[0071] Since Fusarium graminearum and Fusarium laminarum are the dominant bacteria of maize stalk rot in Heilongjiang Province, representative strains were purchased from commercial channels and re-cultivated on PDA plates for 5 days, and then inoculated into sorghum with a 7 mm diameter bacterial plate Grain culture medium (sorghum grains boiled in boiling water for 20 minutes, then transferred to Erlenmeyer flasks for sterilization at 121°C for 45 minutes), inoculated 5 to 7 bacterial plates in each Erlenmeyer flask, cultivated in the dark at 26°C, and shook the Erlenmeyer flasks 1 to 2 times a day. For the second time, the surface of each sorghum grain is covered with mycelium. Then, rinse the sorghum grains with bacteria with sterile water, filter with sterile double-layer gauze, and adjust the concentration of the spore suspension to 1×10 using a hemocytometer. 6 Spores...

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Abstract

The invention discloses an artificial inoculation identification method for stalk rot resistance of corn varieties, which belongs to the technical field of agriculture. The method comprises the following steps: preparation of spore suspension, preparation of corn resistance resources, inoculation and data statistics: wherein the inoculation is conducted, specifically, an injector is used for being1.2-1.7 cm away from the ground, and 1.4-1.6 ml of spore suspension liquid is injected after a needle head of the injector and a stem are inserted into the center of the stem by forming an angle of 45 degrees and retract by 0.18-0.22 cm; pulling out the needle head, and performing normal management and data statistics; performing statistics of data: after inoculation is performed for 25 days, performing statistics of plant morbidity conditions, calculating disease indexes, and determining anti-infection types according to the disease indexes. The method is time-saving, labor-saving, high in inoculation speed and suitable for screening and investigating a large number of corn resistant materials.

Description

technical field [0001] The invention relates to the field of agricultural technology, and more specifically relates to a method for artificial inoculation identification of corn variety resistance to stem rot disease. Background technique [0002] Maize (Zea mays L.) is an important food crop and feed, providing food for 310 million people in more than 20 countries around the world, accounting for 20-30% of the total calories in the human diet. Reliable corn production is necessary to maintain food security and agricultural stability in China. However, maize stalk rot, caused by various pathogens, is one of the most devastating diseases occurring in most maize producing regions of the world. Maize stalk rot is responsible for severe declines in maize yield and quality in many countries and has become one of the major challenges in maize production in China. Breeding and utilizing disease-resistant varieties is the most effective and economical method. The breeding and scr...

Claims

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Application Information

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IPC IPC(8): C12Q1/18A01H1/04
CPCC12Q1/18A01H1/04
Inventor 李永刚扈光辉刘金鑫任洪雷胡少新
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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