Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pharmaceutical application of pawpaw total triterpenes

A technology of total triterpenes and papaya, applied in the field of skin repair, can solve the problems of large individual differences in curative effect, poor patient compliance, and prone to scarring, and achieve good therapeutic effect, promote regeneration and repair, and shorten wound healing time.

Inactive Publication Date: 2020-10-13
CHINA THREE GORGES UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods used for skin wound healing are mainly the application of genetic engineering, the use of bioengineering materials, the application of nanotechnology, nutritional supplementation therapy, etc., but there are large individual differences in curative effect, high price, poor patient compliance, and easy healing after healing. There are many deficiencies such as scars. Traditional Chinese medicine has a long history and rich experience in treating skin diseases, and has created many different dosage forms. In recent years, with the development of science, Chinese medicine extraction has also received good results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pharmaceutical application of pawpaw total triterpenes
  • Pharmaceutical application of pawpaw total triterpenes
  • Pharmaceutical application of pawpaw total triterpenes

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0027] In the following, in combination with specific experimental examples, the effects of papaya total triterpenes on promoting UVB-damaged human skin fibroblast (HDF) cell proliferation, cell migration and inhibiting cell apoptosis will be specifically illustrated.

[0028] experiment method

[0029] 1) Experimental cells

[0030]In this experiment, human skin fibroblasts (HDF) were purchased from Thermo Fisher Scientific (China) Co., Ltd. and cultured in DMEM medium containing 10% fetal bovine serum at 37°C and 5% CO 2 The cells were grown in the incubator, and the cells in the logarithmic growth phase were taken during the experiment.

[0031] 2) method

[0032] (1) Cell culture

[0033] Normal HDF cells were cultured in DMEM medium containing 10% imported fetal bovine serum at a constant temperature of 37°C and 5% CO 2 , Relatively saturated humidity conditions, sterile culture. The growth of the cells was observed daily, and passage was performed when the monolayer...

experiment example 2

[0057] In the following, in combination with specific experimental examples, the effects of total triterpenes of papaya on promoting cell proliferation, cell migration and inhibiting cell apoptosis of UVB-damaged human umbilical vein endothelial cells (HUVECs) will be specifically illustrated.

[0058] experiment method

[0059] 1) Experimental cells

[0060] Cell lines In this experiment, human umbilical vein endothelial cells (HUVECs) were purchased from the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, and cultured in DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 The cells were grown in the incubator, and the cells in the logarithmic growth phase were taken during the experiment.

[0061] 2) method

[0062] (1) Cell culture

[0063] Normal HUVECs were cultured in DMEM medium containing 10% imported fetal bovine serum at a constant temperature of 37°C and 5% CO 2 , Relatively saturated humidity conditions, sterile cul...

experiment example 3

[0085] Below in conjunction with specific experimental examples, it is specifically illustrated that the total triterpenes of papaya obtained in Example 1 of the present invention has an obvious therapeutic effect on rat skin damage caused by surgical cutting.

[0086] experiment method

[0087] 1) Experimental animals

[0088] Animals and feed were purchased from the Experimental Animal Center of China Three Gorges University. There were 50 SPF grade Sprague Dawley (SD) rats, weighing 180-220 g, half male and half male. The experimental animal quality certificate number: SCXK (E) 2017-0061. Animals were kept in a dry, ventilated, and quiet environment in a general-grade animal room, 5 animals / cage. Animal experiments followed the guidelines of the Experimental Animal Ethics Committee of China Three Gorges University, and the experimental research was carried out under its supervision.

[0089] 2) method

[0090] (1) Production of skin injury rat model

[0091] The model i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses pharmaceutical application of pawpaw total triterpenes. The invention specifically comprises application of pawpaw total triterpenes in preparing medicines for promoting cell proliferation and cell migration of human skin fibroblast HDF with UVB injury and inhibiting cell apoptosis, application in preparing medicines for promoting cell proliferation and cell migration of human umbilical vein endothelial cells HUVECs with UVB injury and inhibiting cell apoptosis, application in preparing medicines for treating postoperative cut injury wound healing and application in preparing medicines for treating injured skin regeneration and repair. The pawpaw total triterpenes have a remarkable treatment effect.

Description

technical field [0001] The invention belongs to the technical field of skin restoration, and in particular relates to the preparation process and application of papaya total triterpene hydrogel. Background technique [0002] The skin is the largest organ of the human body. It has many important functions such as metabolism, absorption, protection, temperature regulation, secretion and sensation. It is easily damaged when stimulated by external chemical and physical factors. The healing process after acute skin trauma is very likely to cause scars and wound shrinkage, which affects normal skin function, aesthetics and psychology. Therefore, regeneration and functional restoration of skin after injury has become the focus of clinicians. Especially for patients with full-thickness skin defects, the wound heals mainly through the migration of adjacent skin epithelial cells and the regeneration of stem cells in the remaining skin appendages. If the skin defect area is too large o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/732A61P17/16A61P17/02
CPCA61K36/732A61P17/02A61P17/16
Inventor 贺海波石孟琼廖勇宋利华张媛媛何毓敏卫巧程凡邹坤张继红
Owner CHINA THREE GORGES UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com