Method for extracting astragaloside from fresh astragalus membranaceus

A technology for astragaloside IV and astragalus, which is applied in the field of extracting astragaloside IV, can solve the problems affecting the extraction of astragaloside IV, pollution of macroporous adsorption resin, increase production cost, etc. Effect

Active Publication Date: 2020-10-16
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Fresh astragalus contains more complex chemical components than dried astragalus. In addition to astragaloside IV, it also contains a large number of macromolecular impurities, such as polysaccharides, tannins, proteins, resins, etc. These impurities may form suspended solids or Colloid, difficult to remove, and will pollute the macroporous adsorption resin, seriously affecting the extraction of astragaloside IV
[0006] At present, almost all the raw materials for extracting astragaloside IV reported in the literature are extracted from dried astragalus root or traditional Chinese medicine astragalus slices by crushing into powder. The drying process of astragalus is time-consuming and laborious and prone to mildew. The extraction raw material for extracting astragaloside IV will increase the production cost

Method used

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  • Method for extracting astragaloside from fresh astragalus membranaceus
  • Method for extracting astragaloside from fresh astragalus membranaceus

Examples

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Effect test

Embodiment 1

[0021] In a 5L ultrasonic extraction device, put 1Kg of fresh astragalus roots that have been cleaned and cut into sections, add 5.0L of 80% ethanol solution, adjust the ultrasonic power to 350W, extract at a temperature of 55°C, extract for 40 minutes, extract 3 times, and combine the extracts , Remove ethanol under reduced pressure to obtain a concentrated solution; add 2.0 L of water to the concentrated solution to make a concentrated solution, and filter. Add sodium hydroxide-calcium hydroxide (mass ratio 1:8) mixed alkali to the filtrate, adjust the pH to 13, stir at 45°C for 3.5 hours, let it stand, and filter through a 250-mesh filter; adjust the pH value of the filtrate to 5,40 with dilute hydrochloric acid Under stirring at ℃, add 0.6L chitosan solution to the filtrate, let it stand, and filter with a 500-mesh stainless steel filter. The filtrate was adsorbed with AB-8 macroporous adsorption resin column, eluted with 80% ethanol solution, the eluate was concentrated t...

Embodiment 2

[0023] In a 5L ultrasonic extraction device, put 1Kg of fresh astragalus roots cleaned and cut into sections, add 6.0L of 75% ethanol solution, adjust the ultrasonic power to 300W, extract at 60°C for 60 minutes, extract 3 times, and combine the extracts , Remove ethanol under reduced pressure to obtain a concentrated solution, add 0.5 L of water to the concentrated solution to make a concentrated solution, and filter. Add sodium hydroxide-calcium hydroxide (mass ratio 1:8) mixed alkali to the filtrate, adjust the pH to 13, stir at 50°C for 4 hours, let it stand, and filter through a 200-mesh filter; adjust the pH value of the filtrate to 6 with hydrochloric acid, 45°C Under stirring, add 0.8L chitosan solution in the filtrate, let it stand, and filter with a 500-mesh stainless steel strainer. The filtrate was adsorbed with a D101 macroporous adsorption resin column, eluted with 80% ethanol solution, the eluate was concentrated to a small volume, a precipitate appeared after s...

Embodiment 3

[0025] In a 5L ultrasonic extraction device, put 1Kg of fresh Astragalus roots cleaned and cut into sections, add 8L of 85% ethanol solution, adjust the ultrasonic power to 350W, extract the temperature at 55°C, extract for 40 minutes, extract 3 times, and combine the extracts. Ethanol was removed under reduced pressure to obtain a concentrated solution; 1.5 L of water was added to the concentrated solution to prepare a concentrated solution, which was filtered. Add sodium hydroxide-calcium hydroxide (mass ratio 1:6) mixed alkali to the filtrate, adjust the pH to about 12, stir at 40°C for 4 hours, let it stand, and filter through a 200-mesh filter; adjust the pH value of the filtrate to 5,40 with hydrochloric acid Under stirring at ℃, add 1.0L chitosan solution to the filtrate, let it stand, and filter with a 300-mesh stainless steel filter. The filtrate was adsorbed with HPD-100 macroporous adsorption resin column, eluted with 80% ethanol solution, the eluate was concentrate...

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Abstract

The invention discloses a method for extracting astragaloside from fresh astragalus membranaceus. The method comprises the following steps: cleaning and cutting fresh astragalus membranaceus roots into sections, and extracting with an ethanol solution for multiple times under an ultrasonic condition; merging the extracting solutions, concentrating to remove ethanol, hydrolyzing the concentrated solution with sodium hydroxide and calcium hydroxide, filtering, precipitating; then flocculating and precipitating by using a flocculating agent (chitosan), filtering; removing impurities through a flocculation-precipitation mode and filtering of a filter screen; separating through macroporous adsorption resin, and finally purifying through recrystallization to obtain high-purity astragaloside. Themethod has the advantages of easily available raw materials, low cost, high astragaloside extraction efficiency, high astragaloside purity and simple technique, and is suitable for large-scale industrial production, and the raw materials involved in the extraction process are non-toxic and harmless.

Description

technical field [0001] The invention relates to the extraction of effective components of traditional Chinese medicine, in particular to a method for extracting astragaloside IV from fresh Astragalus membranaceus. Background technique [0002] Astragalus, produced in Shanxi, Inner Mongolia, Gansu, Heilongjiang and other places. Its medicinal use has a history of more than 2,000 years, and it is also one of the traditional Chinese medicinal materials that people often eat. Astragaloside IV, as one of the main active ingredients in Astragalus membranaceus, is a kind of saponins. It has the effects of improving cardiopulmonary function, protecting the liver, inhibiting virus reproduction and tumor growth, and enhancing immunity. [0003] Astragaloside IV, molecular formula: C 41 h 68 o 14 , CAS No.: 83207-58-3, is the main component of astragalosides. Its homologs include acetyl astragaloside Ⅰ (Acetylastragaloside Ⅰ), astragaloside Ⅰ (Astragaloside Ⅰ), isoastragaloside Ⅰ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J53/00
CPCC07J53/004
Inventor 潘继刚黄月君张钧
Owner SHANXI UNIV
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