Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of electrochemical luminescence sensor capable of simultaneously detecting two kinds of sialylated glycans

A sialylation and sensor technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of few sensors and unstable optical signals, so as to improve sensitivity and detection range, facilitate commercialization, and promote precision medicine Effect

Inactive Publication Date: 2020-10-16
CHONGQING MEDICAL UNIVERSITY
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Meso-tetrakis(4-carboxyphenyl)porphine (TCPP), a porphyrin derivative with strong electroluminescence, is rarely used in the construction of sensors due to the instability of the emitted light signal.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of electrochemical luminescence sensor capable of simultaneously detecting two kinds of sialylated glycans
  • Preparation of electrochemical luminescence sensor capable of simultaneously detecting two kinds of sialylated glycans
  • Preparation of electrochemical luminescence sensor capable of simultaneously detecting two kinds of sialylated glycans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Step 1. Disperse the prepared signal probe 1 (MAL-Au@BSA MSs-Luminol) in 1 mL of PBS (pH 7.4) for use.

[0046] Step 2. Disperse the prepared signal probe 2 (SNA-ZIF-8-TCPP) in 1 mL of PBS (pH 7.4) for use.

[0047] Step 3. Add 20 μL of the prepared capture probes (APBA-MMs) to the reaction pool containing 100 μL of different concentrations of α2,3-sialylated glycan and α2,6-sialylated glycan, and then place them at 37°C Incubate for 2 hours.

[0048] Step 4. Afterwards by magnetic separation and washing, it was redissolved to 100 μL. Then, 10 μL of signal probe 1 and signal probe 2 prepared in step 1 and step 2 were added to the above solution, and incubated for 2 hours.

[0049] Step 5. Redissolve to 100 μL by magnetic separation and washing to remove unattached material. Then drop 10 μL of the above solution onto the surface of the electrode, and place it at room temperature to dry.

[0050] Step 6. Place the electrode in 3mL 0.1M PBS (containing 80mM K 2 S 2 o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention also discloses preparation of an electrochemical luminescence sensor capable of simultaneously detecting two kinds of sialylated glycans. According to the invention, a novel luminescentcomposite material Au (at) BSA MSs-Luminol and ZIF-8-TCPP are successfully prepared, and a potential resolution type electrochemical luminescence biosensor is developed for simultaneously detecting alpha2, 3-sialylated glycan and alpha2, 6-sialylated glycan. Luminol and meso-tetrakis (4-carboxyphenyl) porphin (TCPP) can emit optical signals at positive and negative potentials respectively. The formula (I) is shown in the specification. The bovine serum albumin doped gold microspheres (Au-BSA MSs) have a large number of active sites and excellent conductivity, and can be used for immobilizing more biological materials and remarkably improving luminol signals. Zeolite imidazolate skeleton-8 (ZIF-8) has a stable skeleton structure and a rough surface, and can provide more active sites for immobilizing biological materials and stabilize optical signals of meso-tetra (4-carboxyphenyl) porphin in an immobilization manner. Besides, based on the design of a magnetic capture probe 3-aminophenylboronic acid-magnetic microspheres (APBA-MMs), the construction process of the sensor is carried out in a solution, and compared with construction on an electrode, the sensor has a wider linear range.

Description

technical field [0001] The present invention relates to a preparation method and application of an electrochemiluminescent sensor for quantitative detection of α2,3-sialylated glycan and α2,6-sialylated glycan in clinic. The biosensor prepared by the ball-luminol composite material and the zeolite imidazolate skeleton-8-meso-tetrakis(4-carboxyphenyl)porphine as a signal probe belongs to the field of electrochemical detection. Background technique [0002] It has been reported that sialic acid is commonly found on glycoconjugates on the cell surface. The variation of α2,3-sialylation and α2,6-sialylation may be related to the occurrence and progression of various cancers, such as gastric cancer, pancreatic cancer, liver cancer and colon cancer. When cancer cells undergo apoptosis, the sialylated glycans on the cell surface will be released into the blood, which will lead to an increase in the levels of α2,3-sialylated glycans and α2,6-sialylated glycans in the blood. Theref...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48G01N33/574
CPCG01N27/305G01N27/3278G01N27/48G01N33/574G01N33/57488G01N2400/10
Inventor 于超朱启豪毛巍然
Owner CHONGQING MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com