Preparation method and application of tissue engineering cartilage

Abstract

The invention discloses a preparation method of tissue engineering cartilage. The preparation method comprises the following steps of S1, preparing collagen substrates: adding I type collagen and II type collagen into a bacteria-free pipe, adding a high-glucose DMEM culture medium which is precooled at 4 DEG C, performing thorough mixing to obtain a mixture, and performing standing for standby application; S2, preparing mesenchymal stem cells: suspending human mesenchymal stem cells according to the concentration of 1*10<7>cells / cm<2> to an inducing culture medium to obtain a cell suspension;and S3, performing culture preparation: adding the collagen substrates to 1mL of the cell suspension, performing thorough mixing, performing culture overnight, then slowly adding 5mL of the inducing culture medium, continuing to perform culture, performing replacing with a fresh inducing culture solution once every 48 hours, and after 15 days, obtaining the tissue engineering cartilage. The preparation method disclosed by the invention is simple in technological process, and the prepared tissue engineering cartilage can be subjected to shape setting according to actual demands, has good biological mechanics performance and biocompatibility, and has a wide application range.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a method for preparing tissue engineered cartilage. Background technique [0002] Articular cartilage damage caused by trauma, inflammation, degeneration, tumor resection, etc. is a common clinical disease. Due to the lack of vascular tissue in the cartilage, and the cartilage tissue cells are wrapped in matrix components, they cannot migrate to the damaged site to repair the defect, resulting in poor regeneration and repair ability after cartilage damage. If the cartilage damage is not treated in time, it is easy to cause joint snapping , stiffness, increased pain, etc., eventually requiring joint replacement, seriously affecting the quality of life of patients. [0003] Traditional treatment methods include joint lavage, joint debridement, microfracture surgery, periosteum transplantation, cartilage transplantation and autologous chondrocyte transplantation, etc., wh...

AI Technical Summary

Problems solved by technology

For example, joint debridement can only temporarily relieve symptoms, but cannot effectively treat cartilage damage; microfracture surgery can only regenerate fibrocartilage at the defect site, which cannot meet the mechanical requirements of normal cartilage, and there is a hidden danger of later degeneration; autologous cartilage transplantation is better, It is only suitable for the treatment of small cartilage defects; allogeneic cartilage transplantation can be used for repairing larger defects, but the source of donors is limited, and there are risks of infectious diseases and immune rejection

Clinical studies have also shown that chondrocyte transplantation is currently an effective way to repair articular cartilage damage and achieve functional reconstruction; the first-generation transplantation technology needs to use the patient's autologous periosteum to seal the defect position, which increases the damage to the patient and is prone to periosteum hypertrophy. Secondary surgery; the second-generation technology uses collagen / hyaluronic acid membrane instead of autologous periosteum to seal the defect surface, and the transplanted cell suspension may easily cause leakage of chondrocytes, making the surface of new cartilage uneven; the third-generation technology is to directly seal the defect on the collagen membrane. Cells are planted, and fibrin glue is used as a fixative to attach to the cartilage defect in order to form uniform cartilage. However, clinical results show that most of the cartilage generated by this technique is fibrocartilage, and the curative effect is not as stable as that of the first-generation technique. Chondrocytes cannot penetrate the collagen membrane and can only attach and grow on the surface of the collagen membrane. After the material is implanted in the defect site, the cartilage tissue formed is mostly fibrocartilage, which has poorer mechanical properties than normal cartilage and is easy to degenerate.